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Regulation of pathogenicity via envelope stress response in Bordetella pertussis

Research Project

Project/Area Number 23590517
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyorin University

Principal Investigator

HANAWA Tomoko  杏林大学, 医学部, 講師 (80255405)

Co-Investigator(Kenkyū-buntansha) KAMIYA Shigeru  杏林大学, 医学部, 教授 (10177587)
Co-Investigator(Renkei-kenkyūsha) YONEZAWA Hideo  杏林大学, 医学部, 講師 (60453528)
YAMAMOTO Tomoko  千葉大学, 薬学研究科, 教授 (60110342)
Research Collaborator ARMSTRONG Sandra K  ミネソタ大学, 医学研究科, 教授
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords百日咳菌 / 細胞表層 / ストレス応答 / 病原性 / sigmaE / 表層ストレス / RseA / 細胞表層応答 / σE / 病原因子 / アデニレートシクラーゼヘモリジン / 発現
Research Abstract

Cell surface has an important role in the functioning of Bordetella pertussis virulence factors. We found cell-free adenylate cyclase toxin (ACT) was increased by the activation of sigma E, which is alternative sigma subunit of RNA polymerase relating to envelope stress response. In the present study, we investigated the mechanism of increase of cell-free ACT by sigma E activation.
Transcriptions of the genes encoding ACT and its secretion machinery did not altered by activation of sigma E. On the other hand, production of outer membrane vesicle (OMV) including ACT was significantly increased. Different manner of release would provide the stability to ACT resulted in the increase of cell-free ACT. ACT inhibits phagocytosis by macrophages. Activation of sigma E by deletion of RseA was decreased the efficiency of phagocytosis. It is suggested that sigma E-dependent envelope stress response would be one of the regulation system of the pathogenicity of B. pertussis.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (14 results)

All 2014 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results)

  • [Journal Article] Role of Bordetella pertussis RseA in the cell envelope stress response and adenylate cyclase toxin release.2013

    • Author(s)
      Hanawa T, Yonezawa H, Kawakami H, Kamiya S, Armstrong SK.
    • Journal Title

      Pathogens and Disease

      Volume: 69 Pages: 7-20

    • DOI

      10.1111/2049-632x.12061

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of (p)ppGpp in biofilm formation and expression of filamentous structures in Bordetella pertussis.2013

    • Author(s)
      Sugisaki K, Hanawa T, Yonezawa H, Osaki T, Fukutomi T, Kawakami H, Yamamoto T, Kamiya S.
    • Journal Title

      Microbiology

      Volume: 159 Issue: Pt_7 Pages: 1379-1389

    • DOI

      10.1099/mic.0.066597-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] グルタミン酸飢餓環境における百日咳菌III型分泌装置および線毛の発現調節2014

    • Author(s)
      花輪智子、米澤英雄、大崎敬子、蔵田訓、シンシア ザマン、北条史、神谷茂
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Expression of virulence factors under nutrient-limited condition in Bordetella pertussis2013

    • Author(s)
      Tomoko Hanawa, Kentaro Sugisaki, Hideo Yonezawa, Hayato Kawakami, Shigeru Kamiya
    • Organizer
      10th International Symposium on Bordetella
    • Place of Presentation
      Dublin, Ireland
    • Related Report
      2013 Final Research Report
  • [Presentation] 百日咳菌(p)ppGpp欠損株のBiofilm形成および病原因子の発現2013

    • Author(s)
      杉崎健太郎,花輪智子,米澤英雄,大﨑敬子,蔵田訓,北条史,神谷茂
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] Expression of virulence factors under nutrient-limited condition in Bordetella pertussis2013

    • Author(s)
      Tomoko Hanawa, Sugisaki Kentaro, Yonezawa Hideo, Hayato Kawakami, Shigeru Kamiya
    • Organizer
      10th International Symposium on Bordetella.
    • Place of Presentation
      ダブリン(アイルランド)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Biofilm formation of Bordetella pertussis; response to nutritional starvation2012

    • Author(s)
      Tomoko Hanawa, Kentaro Sugisaki, Hideo Yonezawa, Hayato Kawakami, Shigeru Kamiya
    • Organizer
      Biofilms 5
    • Place of Presentation
      Paris, France
    • Related Report
      2013 Final Research Report
  • [Presentation] Implication of the role of stringent response in the expression of adenylate cyclase toxin in Bordetella pertussis2011

    • Author(s)
      Tomoko Hanawa, Kentrao Sugisaki, Hideo Yonezawa, Takako Osaki, Satoshi Kurata, Cynthia Zaman, Shigeru Kamiya
    • Organizer
      The Joint Meeting of the 17th International Symposium on Gnotobiology (ISG) and the 34th Congress of Society for Microbial Ecology and Disease
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Final Research Report
  • [Presentation] Envelope stress response and secretion in Bordetella pertussis2011

    • Author(s)
      Tomoko Hanawa, Hideo Yonezawa, Takako Osaki, Satoshi Kurata, Cynthia Zaman, Sandra Armstrong, and Shigeru Kamiya
    • Organizer
      XIII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Role ofσ^E in the envelope stress response and an implication for the production of toxins in Bordetella pertussis2011

    • Author(s)
      Tomoko Hanawa, Hideo Yonezawa, Shigeru Kamiya, Sandra Armstrong
    • Organizer
      111th general meeting of the American Society for Microbiology
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Envelope stress response and secretion in Bordetella pertussisRole of σE in the envelope stress response and an implication for the production of toxins in Bordetella pertussis.2011

    • Author(s)
      T. Hanawa, H. Yonezawa, S. Kamiya, S. K. Armstrong
    • Organizer
      111th general meeting of the American Society for Microbiology
    • Place of Presentation
      New Orleans, LA, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Envelope stress response and secretion in Bordetella pertussis.2011

    • Author(s)
      T. Hanawa, H. Yonezawa, T. Osaki, S. Kurata, C. Zaman, S. K. Armstrong, and S. Kamiya
    • Organizer
      XIII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] Implication of the role of stringent response in the expression of adenylate cyclase toxin in Bordetella pertussis.2011

    • Author(s)
      T. Hanawa, K. Sugisaki, H. Yonezawa, T. Osaki, S. Kurata, C. Zaman, S. Kamiya
    • Organizer
      The Joint Meeting of the 17th International Symposium on Gnotobiology (ISG) and the 34th Congress of Society for Microbial Ecology and Disease
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] 百日咳菌の病原遺伝子発現に及ぼす栄養枯渇影響

    • Author(s)
      中里仁美、花輪智子、福冨俊之、海保房夫、神谷茂
    • Organizer
      第57回日本薬学会関東支部大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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