Integrated analysis of translocation of Pseudomonas aeruginosa from the colonized gastrointestinal tract
| Project/Area Number |
23590523
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kyoto Pharmaceutical University |
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Principal Investigator |
GOTOH Naomasa 京都薬科大学, 薬学部, 教授 (30121560)
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| Co-Investigator(Kenkyū-buntansha) |
OKUDA Jun 香川県立保健医療大学, 保健医療学部, 教授 (90334276)
MINAGAWA Shu 京都薬科大学, 薬学部, 助教 (50445962)
HAYASHI Naoki 京都薬科大学, 薬学部, 助教 (70707463)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 緑膿菌 / バクテリアルトランスロケーション / ムチン層 / シデロフォア / mucD遺伝子 / 鞭毛 / トランスロケーション / 比較ゲノム解析 / セリンプロテアーゼ / III型分泌機構 / 上皮細胞層 / ExoS / MucD / 細胞層透過 |
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| Research Abstract |
In this study, our findings provided at least three new mechanisms for translocation of Pseudomonas aeruginosa from the colonized gastrointestinal tract: (i) the pyoverdine synthesis gene (pvdE) facilitates penetration of P. aeruginosa through the epithelial cell barrier depending on regulating the expression of the exoS gene, impairs the defense function of tight junctions; (ii) P. aeruginosa can degrade interleukin-8 (IL-8) following the expression of the exoS and mucD gene; (iii) P. aeruginosa can penetrate the mucus layer using the flagellar motility and the mucin degradation. These results revealed that functions of P. aeruginosa can penetrate mucus layer and epithelial cells junction, which serve as important barriers in the intestinal epithelial tissue, and a survival strategy of P. aeruginosa to the host immune system.
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Pseudomonas aeruginosa MucD protease mediate keratitis by inhibition of neutrophil recruitment and bacterial survival.2014
Author(s)
Mochizuki, Y., Suzuki, T., Oka, N., Zhang, Y., Hayashi, Y., Hayashi, N., Gotoh, N., and Ohashi, Y.
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Journal Title
Invest. Ophthalmol. Vis. Sci.
Volume: 55
Issue: 1
Pages: 240-246
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A novel effector secretion mechanism based on proton-motive force-dependent type III secretion apparatus rotation.2013
Author(s)
Ohgita, T., Hayashi, N., Hama, S., Tsuchiya, H., Gotoh, N., and Kogure, K.
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Journal Title
FASEB. J.
Volume: 27
Issue: 7
Pages: 2862-2872
DOI
Related Report
Peer Reviewed
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[Journal Article] Interplay of flagellar motility and mucin degradation by MucD stimulates the association of Pseudomonas aeruginosa with human epithelial colorectal adenocarcinoma (Caco-2) cells.2013
Author(s)
Hayashi, N., Matsukawa, M., Horinishi, Y., Nakai, K., Shoji, A., Yoneko, Y., Yoshida, S., Minagawa, S., Gotoh, N.
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Journal Title
J. Infect. Chemother.
Volume: 19
Issue: 2
Pages: 305-315
DOI
Related Report
Peer Reviewed
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[Journal Article] Potent in vitro antibacterial activity of DS-8587, a new generation of broad spectrum quinolone, against Acinetobacter baumannii.2013
Author(s)
Higuchi, S., Onodera, Y., Chiba, M., Hoshino, K., and Gotoh, N.
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Journal Title
Antimicrob. Agents Chemother.
Volume: 57
Issue: 4
Pages: 1978-1981
DOI
Related Report
Peer Reviewed
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[Journal Article] Neutrophil-derived tumor necrosis factor-α contributes to acute wound healing promoted by N-(3-oxododecanoyl)-l-homoserine lactone from Pseudomonas aeruginosa.2013
Author(s)
Kanno, E., Kawakami, K, Miyairi, S., Tanno, H., Otomaru, H., Hatanaka, A., Sato, S., Ishii, K., Hayashi, D., Shibuya, N., Imai, Y., Gotoh, N., Maruyama, R., and Tachi, M.
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Journal Title
J. Dermatol. Sci.
Volume: 70
Issue: 2
Pages: 130-138
DOI
Related Report
Peer Reviewed
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[Journal Article] Complementation of the exoS gene in the pvdE pyoverdine synthesis gene-deficient mutant of Pseudomonas aeruginosa results in recovery of the pvdE gene-mediated penetration through the intestinal epithelial cell barrier but not the pvdE-mediated virulence in silkworms.2012
Author(s)
Okuda, J., Okamoto, M., Hayashi, N., Sawada, S., Minagawa, S., Gotoh, N.
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Journal Title
J. Infect. Chemother.
Volume: 18
Issue: 6
Pages: 332-340
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] In vitro and in vivo antibacterial activity of modithromycin against streptococci and Haemophilus influenzae.2011
Author(s)
Sato, T., Kawai, Y., Matsuda, H., Tateda, K., Kimura, S., Ishii, Y., Yamaguchi, K., Gotoh, N.
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Journal Title
J. Antimicrob. Chemother.
Volume: 66
Issue: 7
Pages: 1547-1554
DOI
Related Report
Peer Reviewed
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