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Study on structural biology of novel fibronectin-binding proteins of a pathogenic bacterium

Research Project

Project/Area Number 23590524
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOkayama University of Science

Principal Investigator

KATAYAMA Seiichi  岡山理科大学, 理学部, 准教授 (70169473)

Co-Investigator(Kenkyū-buntansha) HITSUMOTO Yasuo  岡山理科大学, 理学部, 教授 (90136333)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
KeywordsClostridium perfringens / fibronectin (Fn) / Fn-binding protein / Fn III1-C-binding site / ウェルシュ菌 / ガス壊疽 / フィブロネクチン (Fn) / Fn結合タンパク質 / 国際情報交流 / フィブロネクチン(Fn)
Research Abstract

Clostridium perfringens is a pathogenic bacterium, causing gas gangrene and food poisoning in humans and animals. During the beginning of the infection, the bacteria formed colonies after the adhesion to wounds and epithelial cells of intestine. C. perfringens have proteins, FbpA (25 kDa, 220 aa) and FbpB (66 kDa, 575 aa) that bind to fibronectin in human. Therefore, this bacterium is thought to colonize after its Fn-binding.
In this study, we indicated that Fn molecules bound to the C-terminal region of FbpA. An improved method of purification of recombinant FbpA was established to get a great quantity of the protein for crystallization. In addition, we found that both FbpA and FbpB could bind to the surface of C. perfringens cell. The studies on the structures and functions of the Fbps might lead to the development of protection against C. perfringens infection in the future.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (34 results)

All 2014 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (32 results)

  • [Journal Article] Adhesive properties of Clostridium perfringens to extracellular matrix proteins collagens and fibronectin2014

    • Author(s)
      Yasuo Hitsumoto, Naomi Mrita, Ryousuke Yamazoe, Mika Tagomori, Tsutom Yamasaki, Seiichi Katayama
    • Journal Title

      Anaerobe

      Volume: 25 Pages: 67-71

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adhesive properties of Clostridium perfringens to extracellular matrix proteins collgens and fibronectin2014

    • Author(s)
      Yasuo Hitsumoto, Naomi Morita, Ryousuke Yamazoe, Mika Tagimori, Tsutomu Yamasaki, Seiichi Katayama
    • Journal Title

      Anaerobe

      Volume: 25 Pages: 67-71

    • DOI

      10.1016/j.anaerobe.2013.11.002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウェルシュ菌の菌体表層にみられたフィブロネクチン結合タンパク質をコードする遺伝子の同定2014

    • Author(s)
      加藤早苗、山崎勤、成谷宏文、櫃本泰雄、片山誠一
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2014-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Clostridium perfringensのfibronectin-binding proteinが認識するIII_1-Cを表出する血清フィブロネクチン2014

    • Author(s)
      山崎勤、勢家洋子、岡田真理子、片山誠一、櫃本泰雄
    • Organizer
      第24回生物試料分析科学会年次学術集会
    • Place of Presentation
      鈴鹿医療科学大学千代崎キャンパス(三重・鈴鹿市)
    • Year and Date
      2014-03-01
    • Related Report
      2013 Final Research Report
  • [Presentation] ウェルシュ菌の菌体表層にみられたフィブロネクチン結合タンパク質をコードする遺伝子の同定2014

    • Author(s)
      加藤早苗、山崎勤、成谷宏文、櫃本泰雄、片山誠一
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Clostridium perfringensのfibronectin-binding proteinが認識するIII1-Cを表出する血清フィブロネクチン2014

    • Author(s)
      山崎勤、勢家洋子、岡田真理子、片山誠一、櫃本泰雄
    • Organizer
      第24回生物試料分析科学会年次総会
    • Place of Presentation
      鈴鹿医療科学大学千代崎キャンパス(三重)
    • Related Report
      2013 Annual Research Report
  • [Presentation]2013

    • Author(s)
      Yasuo Hitsumoto, Naomi Morita, Ryusuke Yamazoe, Mika Tagomori, Hatsuo Yamashita, Tsutomu Yamasaki, Seiichi Katayama
    • Organizer
      8th International Conference on the Molecular biology and Pathogenesis on the Clostridia
    • Place of Presentation
      Sea Temple Spa & Resort, (Palm Cove, Australia)
    • Year and Date
      2013-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Fibronectin (Fn)-binding proteins of Clostridium perfringens recogniszes III_1-C-expressing serum Fn2013

    • Author(s)
      Tsutomu Yamasaki, Yoko Seike, Hatsuo Yamashita, Seiichi Katayama, Yasuo Hitsumoto
    • Organizer
      8th International Conference on the Molecular biology and Pathogenesis on the Clostridia
    • Place of Presentation
      Sea Temple Spa & Resort, (Palm Cove, Australia)
    • Year and Date
      2013-10-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Determination of binding site for Clostridium perfringens on fibronectin2013

    • Author(s)
      Seiichi Katayama, Mika Tagomori, Hatsuo Yamashita, Tsutomu Yamasaki, Hirofumi Nariya, Mariko Okada, Mariko Watanabe, Yasuo Hitsumoto
    • Organizer
      8th International Conference on the Molecular biology and Pathogenesis on the Clostridia
    • Place of Presentation
      Sea Temple Spa & Resort, (Palm Cove, Australia)
    • Year and Date
      2013-10-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Clostridium perfringens由来フィブロネクチン結合タンパク質FbpAの好中球遊走に与える影響2013

    • Author(s)
      山下初津雄、山崎勤、片山誠一、櫃本泰雄
    • Organizer
      第8回生物試料分析科学会中国四国支部学術集会
    • Place of Presentation
      広島国際大学広島キャンパス(広島市)
    • Year and Date
      2013-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] ウェルシュ菌フィブロネクチン(Fn)結合タンパク質FbpAのFn III_1-C結合ドメインの探索2013

    • Author(s)
      岡田麻里、櫃本泰雄、片山誠一
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Year and Date
      2013-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Clostridium perfringens由来Fibronectin結合タンパク(Fbp)の好中球遊走抑制活性2013

    • Author(s)
      中嶋一秋、山下初津雄、森田奈緒美、山崎勤、片山誠一、櫃本泰雄
    • Organizer
      第23回生物試料分析科学会年次学術集会
    • Place of Presentation
      新梅田研修センター(大阪市)
    • Year and Date
      2013-02-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Fibronectin (Fn)-binding proteins of Clostridium perfringens recognizes III1-C-expressing serum Fn2013

    • Author(s)
      Tsutom Yamasaki, Yoko Seike, Hatsuo Yamashita, Seiichi Katayama, Yasuo Hitsumoto
    • Organizer
      8th International Conference on the Molecular Biology and Pathogenesis on the Clostridia
    • Place of Presentation
      Sea Temple Spa & Resort, Palm Cove, Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] Adheasive Properties of Clostridium perfringens to extracellular matrix proteins, collagens and fibronectin2013

    • Author(s)
      Yasuo Hitsumoto, Naomi Morita, Ryousuke Yamazoe, Mika Tagomori, Hatsuo Yamashita, Tsuotmu Yamasaki, Seiichi Katayama
    • Organizer
      8th International Conference on the Molecular Biology and Pathogenesis on the Clostridia
    • Place of Presentation
      Sea Temple Spa & Resort, Palm Cove, Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] Determination of binding site for Clostridium perfringens on fibronectin2013

    • Author(s)
      Seiichi Katayama, Mika Tagomori, Hatsuo Yamashita, Tsutom Yamasaki, Hirofumi Nariya, Mariko Okada, Mariko Watanabe, Yasuo Hitsumoto
    • Organizer
      8th International Conference on the Molecular Biology and Pathogenesis on the Clostridia
    • Place of Presentation
      Sea Temple Spa & Resort, Palm Cove, Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] ウェルシュ菌の菌体表層にみられる新規フィブロネクチン結合タンパク質2013

    • Author(s)
      加藤早苗、山崎勤、成谷宏文、櫃本泰雄、片山誠一
    • Organizer
      第66回日本細菌学会中国・四国支部総会
    • Place of Presentation
      広島国際大学メディアホール(広島・呉)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Clostridium perfringens由来フィブロネクチン結合タンパクFbpAの好中球遊走に与える影響2013

    • Author(s)
      山下初津雄、山崎勤、片山誠一、櫃本泰雄
    • Organizer
      第8回生物試料分析科学会中国四国支部学術集会
    • Place of Presentation
      広島国際大学広島キャンパス300教室(広島)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Clostridium perfringens由来Fibronectin結合タンパク(Fbp)の好中球遊走抑制活性2013

    • Author(s)
      中嶋一秋、山下初津雄、森田奈緒美、山崎 勤、片山誠一、櫃本泰雄
    • Organizer
      第23回生物試料分析科学会年次学術集会
    • Place of Presentation
      大阪府大阪市
    • Related Report
      2012 Research-status Report
  • [Presentation] ウェルシュ菌フィブロネクチン(Fn)結合タンパク質FbpAのFn III1-C結合ドメインの探索2013

    • Author(s)
      岡田麻里、櫃本泰雄、片山誠一
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉県千葉市
    • Related Report
      2012 Research-status Report
  • [Presentation] ウェルシュ菌の菌体表層にみられる新規フィブロネクチン結合タンパク質2012

    • Author(s)
      加藤早苗、山崎勤、成谷宏文、櫃本泰雄、片山誠一
    • Organizer
      第66回日本細菌学会中国・四国支部総会
    • Place of Presentation
      広島国際大学メディアホール(呉市)
    • Year and Date
      2012-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] ウェルシュ菌フィブロネクチン結合タンパク質FbpBのフィブロネクチン結合ドメインの探索2012

    • Author(s)
      岡田麻里、萬谷悠太、片山誠一、櫃本泰雄
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール他(長崎市)
    • Year and Date
      2012-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Clostridium perfringens由来フィブロネクチン結合タンパクFbpBの生物活性2012

    • Author(s)
      森田奈緒美、田籠美華、山崎勤、片山誠一、櫃本泰雄
    • Organizer
      第7回生物試料分析科学会中国四国支部学術集会
    • Place of Presentation
      島根県出雲市
    • Related Report
      2012 Research-status Report
  • [Presentation] Clostridium perfringens上フィブロネクチンレセプターの認識するフィブロネクチン分子エピトープの決定2012

    • Author(s)
      田籠美華、森田奈緒美、山崎勤、片山誠一、櫃本泰雄
    • Organizer
      第7回生物試料分析科学会中国四国支部学術集会
    • Place of Presentation
      島根県出雲市
    • Related Report
      2012 Research-status Report
  • [Presentation] ウェルシュ菌フィブロネクチン結合タンパク質FbpBのフィブロネクチン結合ドメインの探索2012

    • Author(s)
      岡田 麻里、萬谷 悠太、片山 誠一、櫃本 泰雄
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール他(長崎市)
    • Related Report
      2011 Research-status Report
  • [Presentation] ウェルシュ菌フィブロネクチン(Fn)結合タンパク質FbpBのFn結合部位の探索2011

    • Author(s)
      萬谷悠太、片山誠一、櫃本泰雄
    • Organizer
      第64回日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2011-10-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 好中球走化性に対するヒトFibronectin (Fn)とウェルシュ菌由来Fn結合タンパク(Fbp)の影響2011

    • Author(s)
      洲脇晃平,中嶋一秋,森田奈緒美,片山誠一,櫃本泰雄
    • Organizer
      第6回生物試料分析学会中四国支部学術集会
    • Place of Presentation
      岡山理科大学(岡山市)
    • Year and Date
      2011-10-22
    • Related Report
      2013 Final Research Report
  • [Presentation] ウェルシュ菌フィブロネクチン(Fn)結合タンパク質のFn III_1-C領域への結合2011

    • Author(s)
      萬谷悠太,岡田麻里,片山誠一,森田奈緒美,田籠美華,櫃本泰雄
    • Organizer
      第6回生物試料分析学会中四国支部学術集会
    • Place of Presentation
      岡山理科大学(岡山市)
    • Year and Date
      2011-10-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Interaction of Clostridium perfringens with matrix proteins, fibronectin and collagen2011

    • Author(s)
      Yasuo Hitsumoto, Yusuke Nakamura, Ryusuke Yamazoe, Naomi Morita, Mika Tagomori, and Seiishi Katayama
    • Organizer
      13th International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Clostridium perfringens上フィブロネクチンレセプターの認識するフィブロネクチン分子エピトープの決定2011

    • Author(s)
      田籠美華、森田奈緒美、山崎勤、片山誠一、櫃本泰雄
    • Organizer
      第7回生物試料分析科学会中国四国支部学術集会
    • Place of Presentation
      島根大学医学部(出雲市)
    • Year and Date
      2011-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Clostridium perfringens由来フィブロネクチン結合タンパクFbpBの生物活性2011

    • Author(s)
      森田奈緒美、田籠美華、山崎勤、片山誠一、櫃本泰雄
    • Organizer
      第7回生物試料分析科学会中国四国支部学術集会
    • Place of Presentation
      島根大学医学部(出雲市)
    • Year and Date
      2011-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Interaction of Clostridium perfringens with matrix proteins,fibronectin and collagen2011

    • Author(s)
      Y. Hitsumoto, Y. Nakamura, R. Yamazoe, N. Morita, M. Tagomori, and S. Katayama
    • Organizer
      13th International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2011 Research-status Report
  • [Presentation] ウェルシュ菌フィブロネクチン(Fn)結合タンパク質のFn III1-C領域への結合2011

    • Author(s)
      萬谷 悠太, 岡田 麻里, 片山 誠一, 森田 奈緒美, 田籠 美華, 櫃本 泰雄
    • Organizer
      第6回生物試料分析学会 中四国支部学会
    • Place of Presentation
      岡山理科大学(岡山市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 好中球走化性に対するヒトFibronectin(Fn)とウェルシュ菌由来Fn結合タンパク(Fbp) の影響2011

    • Author(s)
      洲脇 晃平, 中嶋 一秋, 森田 奈緒美, 片山 誠一, 櫃本 泰雄
    • Organizer
      第6回生物試料分析学会 中四国支部学会
    • Place of Presentation
      岡山理科大学(岡山市)
    • Related Report
      2011 Research-status Report
  • [Presentation] ウェルシュ菌フィブロネクチン(Fn)結合タンパク質FbpBのFn結合部位の探索2011

    • Author(s)
      萬谷 悠太、片山 誠一、櫃本 泰雄
    • Organizer
      第64回日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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