Study on structural biology of novel fibronectin-binding proteins of a pathogenic bacterium
| Project/Area Number |
23590524
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Okayama University of Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HITSUMOTO Yasuo 岡山理科大学, 理学部, 教授 (90136333)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | Clostridium perfringens / fibronectin (Fn) / Fn-binding protein / Fn III1-C-binding site / ウェルシュ菌 / ガス壊疽 / フィブロネクチン (Fn) / Fn結合タンパク質 / 国際情報交流 / フィブロネクチン（Fn) |
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| Research Abstract |
Clostridium perfringens is a pathogenic bacterium, causing gas gangrene and food poisoning in humans and animals. During the beginning of the infection, the bacteria formed colonies after the adhesion to wounds and epithelial cells of intestine. C. perfringens have proteins, FbpA (25 kDa, 220 aa) and FbpB (66 kDa, 575 aa) that bind to fibronectin in human. Therefore, this bacterium is thought to colonize after its Fn-binding.
In this study, we indicated that Fn molecules bound to the C-terminal region of FbpA. An improved method of purification of recombinant FbpA was established to get a great quantity of the protein for crystallization. In addition, we found that both FbpA and FbpB could bind to the surface of C. perfringens cell. The studies on the structures and functions of the Fbps might lead to the development of protection against C. perfringens infection in the future.
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Report
(4 results)
Research Products
(34 results)
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[Presentation]2013
Author(s)
Yasuo Hitsumoto, Naomi Morita, Ryusuke Yamazoe, Mika Tagomori, Hatsuo Yamashita, Tsutomu Yamasaki, Seiichi Katayama
Organizer
8th International Conference on the Molecular biology and Pathogenesis on the Clostridia
Place of Presentation
Sea Temple Spa & Resort, (Palm Cove, Australia)
Year and Date
2013-10-25
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