Regulation of virulence gene expressions in high-virulent lineages of enterohemorrhagic E. coli

• Project/Area Number: 23590528
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: IYODA SUNAO 国立感染症研究所, 細菌第一部, 主任研究官 (70300928)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 腸管出血性大腸菌 / LEE / 発現制御

Research Abstract

We examined the distribution of clades and stx subtypes of enterohemorrhagic E. coli (EHEC) isolates, which were collected in Japan to assess their public health significance. Using epidemiologically unrelated EHEC O157:H7 isolates from more than 250 hemolytic uremic syndrome (HUS) patients and 350 asymptomatic carriers (designated as ACs hereafter), all of which exhibited distinct pulsed-field gel electrophoresis patterns, we observed that diffrent clades with particular stx subtypes were associated with HUS and ACs, respectively.

Research Products

• [Journal Article] A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli (2014)
  • Author(s): Sudo N, Soma A, Muto A, Iyoda S, Suh M, Kurihara N, Abe H, Tobe T, Ogura Y, Hayashi T, Kurokawa K, Ohnishi M, Sekine Y
  • Journal Title: J Gen Appl Microbiol Volume: 60 Pages: 44-50
  • NAID: 130004802264
  • Peer Reviewed
• [Journal Article] A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli. (2014)
  • Author(s): Sudo N, Soma A, Muto A, Iyoda S, Suh M, Kurihara N, Abe H, Tobe T, Ogura Y, Hayashi T, Kurokawa K, Ohnishi M, Sekine Y.
  • Journal Title: J Gen Appl Microbiol. Volume: 60 Pages: 44-50
  • NAID: 130004802264
  • Peer Reviewed
• [Journal Article] EspO1-2 regulates EspM2-mediated RhoA activity to stabilize formation of focal adhesions in enterohemorrhagic Escherichia coli-infected host cells. (2013)
  • Author(s): Morita-Ishihara T, Miura M, Iyoda S, Izumiya H, Watanabe H, Ohnishi M, Terajima J.
  • Journal Title: PLoS One Volume: 8 Issue: 2 Pages: e55960-e55960
  • DOI: 10.1371/journal.pone.0055960
  • Peer Reviewed
• [Journal Article] EHEC Study Group. Molecular characterization reveals three distinct clonal groups among clinical shiga toxin-producing Escherichia coli strains of serogroup O103 (2012)
  • Author(s): Iguchi A, Iyoda S, Ohnishi M
  • Journal Title: J Clin Microbiol Volume: 50 Pages: 2894-900
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals (2012)
  • Author(s): Miyashita A, Iyoda S, Ishii K, Hamamoto H, Sekimizu K, Kaito C
  • Journal Title: FEMS Microbiol Lett Volume: 333 Pages: 59-68
  • Peer Reviewed
• [Journal Article] Variation in stress resistance patterns among stx genotypes and genetic lineages of shiga toxin-producing Escherichia coli O157 (2012)
  • Author(s): Lee K, French NP, Jones G, Hara-Kudo Y, Iyoda S, Kobayashi H, Sugita-Konishi Y, Tsubone H, Kumagai S
  • Journal Title: Appl Environ Microbiol Volume: 78 Pages: 3361-8
  • Peer Reviewed
• [Journal Article] Molecular characterization reveals three distinct clonal groups among clinical shiga toxin-producing Escherichia coli strains of serogroup O103. (2012)
  • Author(s): Iguchi A, Iyoda S, Ohnishi M; EHEC Study Group
  • Journal Title: J Clin Microbiol. Volume: 50 Pages: 2894-2900
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide O-antigen of enterohemorrhagic Escherichia coli O157:H7 is required for killing both insects and mammals (2012)
  • Author(s): Atsushi Miyashita, et al.
  • Journal Title: FEMS Microbiology Letters Volume: 333 Issue: 1 Pages: 59
  • DOI: 10.1111/j.1574-6968.2012.02599.x
  • URL: https://pure.teikyo.jp/en/publications/cbf7586d-87d8-4c20-971f-26cfd07e9a06
  • Peer Reviewed
• [Journal Article] Variation in stress resistance patterns among stx genotypes and genetic lineages of shiga toxin-producing Escherichia coli O157. (2012)
  • Author(s): Lee K, French NP, Jones G, Hara-Kudo Y, Iyoda S, Kobayashi H, Sugita-Konishi Y, Tsubone H, Kumagai S.
  • Journal Title: Appl Environ Microbiol. Volume: 78 Issue: 9 Pages: 3361-3368
  • DOI: 10.1128/aem.06646-11
  • Peer Reviewed
• [Journal Article] Coordinate control of the locus of enterocyte effacement and enterohemolysin genes by multiple common virulence regulators in enterohemorrhagic Escherichia coli (2011)
  • Author(s): Iyoda S, Honda N, Saitoh T, Shimuta K, Terajima J, Watanabe H, Ohnishi M
  • Journal Title: Infect Immun Volume: 79 Pages: 4628-37
  • Peer Reviewed
• [Journal Article] Multivariate analyses revealed distinctive features differentiating human and cattle isolates of Shiga toxin-producing Escherichia coli O157 in Japan (2011)
  • Author(s): Lee K, French NP, Hara-Kudo Y, Iyoda S, Kobayashi H, Sugita-Konishi Y, Tsubone H, Kumagai S
  • Journal Title: J Clin Microbiol Volume: 49 Pages: 1495-500
  • Peer Reviewed
• [Journal Article] EHEC Study Group. Emergence of a novel Shiga toxin-producing Escherichia coli O serogroup cross-reacting with Shigella boydii type 10 (2011)
  • Author(s): Iguchi A, Iyoda S, Seto K, Ohnishi M
  • Journal Title: J Clin Microbiol Volume: 49 Pages: 3678-80
  • Peer Reviewed
• [Journal Article] Coordinate control of the locus of enterocyte effacement and enterohemolysin genes by multiple common virulence regulators in enterohemorrhagic Escherichia coli. (2011)
  • Author(s): Iyoda S, Honda N, Saitoh T, Shimuta K, Terajima J, Watanabe H, Ohnishi M.
  • Journal Title: Infect Immun. Volume: 79 Pages: 4628-4637
  • Peer Reviewed
• [Journal Article] Multivariate analyses revealed distinctive features differentiating human and cattle isolates of Shiga toxin-producing Escherichia coli O157 in Japan. (2011)
  • Author(s): Lee K, French NP, Hara-Kudo Y, Iyoda S, Kobayashi H, Sugita-Konishi Y, Tsubone H, Kumagai S.
  • Journal Title: J Clin Microbiol. Volume: 49 Pages: 1495-1500
  • Peer Reviewed
• [Journal Article] Emergence of a novel Shiga toxin-producing Escherichia coli O serogroup cross-reacting with Shigella boydii type 10. (2011)
  • Author(s): Iguchi A, Iyoda S, Seto K, Ohnishi M; EHEC Study Group.
  • Journal Title: J Clin Microbiol. Volume: 49 Pages: 3678-3680
  • Peer Reviewed
• [Journal Article] Variation in Stress Resistance Patterns among stx Genotypes and Genetic Lineages of Shiga Toxin-Producing Escherichia coli O157. (2011)
  • Author(s): Lee K, French NP, Jones G, Hara-Kudo Y, Iyoda S, Kobayashi H, Sugita-Konishi Y, Tsubone H, Kumagai S.
  • Journal Title: Appl Environ Microbiol. Volume: 78 Pages: 3361-3368
  • Peer Reviewed
• [Presentation] 重症者由来LEE非保有型EHECの病原性因子の解析 (2013)
  • Author(s): 伊豫田淳、石原朋子、勢戸和子、泉谷秀昌、小西典子、甲斐明美、中嶋洋、木全恵子、磯部順子、大西真
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-18
• [Presentation] EHECワーキンググループ、腸管出血性大腸菌感染症の現状と保有する接着因子について (2012)
  • Author(s): 伊豫田淳、石原朋子、寺嶋淳、大西真
  • Organizer: 第95回日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-12
• [Presentation] Regulatory network of virulence-related gene expressions in enterohemorrhagic Escherichia coli (2012)
  • Author(s): 伊豫田淳
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-27
• [Presentation] 腸管出血性大腸菌感染症の現況と保有する接着因子について (2012)
  • Author(s): 伊豫田淳，石原朋子，寺嶋淳，大西真，EHECワーキンググループ
  • Organizer: 第95回日本細菌学会関東支部総会
  • Place of Presentation: 東京
• [Presentation] Regulatory network of virulence-related gene expressions in enterohemorrhagic Escherichia coli. (2012)
  • Author(s): Sunao Iyoda
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市・長崎新聞文化ホール