Sequence-based typing of Legionella pneumophila isolates in Japan

• Project/Area Number: 23590530
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: AMEMURA-MAEKAWA Junko 国立感染症研究所, 細菌第一部, 主任研究官 (20238843)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: レジオネラ / Legionella pneumophila / sequence-based typing / 遺伝子型別 / 浴槽水 / 冷却塔水 / 土壌 / 遺伝子型 / SBT / 臨床分離株 / Seqence-based typing / モノクローナル抗体

Research Abstract

Legionella pneumophila (Lp), the causative agent of Legionnaires' disease, grows in the bath water. Over half of Legionella pneumonia patients, however, do not use the bathing facility, and in most such cases, the sources of infection are not clear. I examined the genotypes of Lp strains derived from various environments: cooling tower water, soil, fountains, and shower water as well as bath water, in order to compare the genotypes of Lp strains derived from patients. The genotypes of Lp strains derived from patients who were suspected to infect Lp through bath water were belonged to the genotype groups including most Lp strains derived from bath water. The genotypes of Lp strains derived from patients whose sources of infection were unknown were belonged to the genotype groups including Lp strains derived from environments other than bath water. Therefore inspection and hygiene management of various environments other than bathing facilities as the source of infection are important.

Research Products

• [Journal Article] A case of pneumonia caused by Legionella pneumophila serogroup 12 and treated successfully with imipenem (2014)
  • Author(s): Nishizuka M, Suzuki H, Ara T, Watanabe M, Morita M, Sato C, Tsuchida F, Seto J, Amemura-Maekawa J, Kura F, Takeda H
  • Journal Title: J Infect Chemother Volume: 20 Pages: 390-3
  • Peer Reviewed
• [Journal Article] 患者及び浴場施設検体から複数血清群のLegionella pneumophilaが分離された事例について (2012)
  • Author(s): 市原祥子・江藤良樹・濱﨑光宏・村上光一・竹中重幸・堀川和美、西原千鶴子、荒牧明世、前川純子
  • Journal Title: 福岡県保健環境研究所年報 Volume: 第39号 Pages: 76-80
  • Peer Reviewed
• [Journal Article] Working Group for Legionella in Japan. Distribution of monoclonal antibody subgroups and sequence-based types among Legionella pneumophila serogroup 1 isolates derived from cooling tower water, bathwater, and soil in Japan (2012)
  • Author(s): Amemura-Maekawa J, Kikukawa K, Helbig JH, Kaneko S, Suzuki-Hashimoto A, Furuhata K, Chang B, Murai M, Ichinose M, Ohnishi M, Kura F
  • Journal Title: Appl Environ Microbiol Volume: 12 Pages: 4263-70
  • Peer Reviewed
• [Journal Article] Distribution of monoclonal antibody subgroups and sequence-based types among Legionella pneumophila serogroup 1 isolates derived from cooling tower water, bathwater, and soil in Japan. (2012)
  • Author(s): Amemura-Maekawa J, Kikukawa K, Helbig JH, Kaneko S, Suzuki-Hashimoto A, Furuhata K, Chang B, Murai M, Ichinose M, Ohnishi M, Kura F; Working Group for Legionella in Japan
  • Journal Title: Appl. Environ. Microbiol. Volume: 12 Issue: 12 Pages: 4263-4270
  • DOI: 10.1128/aem.06869-11
  • Peer Reviewed
• [Presentation] 新しいneuAプライマーによるLegionella pneumophila臨床分離株のsequence-based typing (SBT) (2014)
  • Author(s): 前川純子、倉文明、渡辺祐子、金谷潤一、磯部順子、田中忍、中嶋洋、吉野修司、大西真
  • Organizer: 第88回日本感染症学会
  • Place of Presentation: 福岡
• [Presentation] Identification of Legionella pneumophila subspecies in clinical environmental isolates in Japan using the microplate DNA-DNA hybridization method (2013)
  • Author(s): Junko Amemura-Maekawa, Michiko Koyano, Toshio Yamazaki, Miyo Murai, Makoto Ohnishi, and Fumiaki Kura
  • Organizer: The 8th international conference on Legionella
  • Place of Presentation: Melbourne Australia
• [Presentation] Close genetic relationship between Legionella pneumophila serogroup 1 isolates from sputum specimens and puddles on roads by sequence-based typing (2013)
  • Author(s): Jun-ichi Kanatani, Junko Isobe, Keiko Kimata, Tomoko Shima, Miwako Sshimuzu, Junko Amemura-Maekawa, Fumiaki Kura, Tetsutaro Sata, Masanori Watahiki
  • Organizer: The 8th international conference on Legionella
  • Place of Presentation: Melbourne Australia
• [Presentation] Legionella pneumophila臨床分離株の遺伝子解析による分類 (2013)
  • Author(s): 前川純子、倉文明、渡辺祐子、磯部順子、田中忍、中嶋洋、吉野修司、大西真
  • Organizer: 第87回日本感染症学会
  • Place of Presentation: 横浜
• [Presentation] Legionella pneumophila血清群1環境分離株の遺伝子解析による分類 (2013)
  • Author(s): 前川純子、倉文明、渡辺祐子、磯部順子、田中忍、中嶋洋、吉野修司、大西真
  • Organizer: 第87回日本感染症学会
  • Place of Presentation: 横浜
• [Presentation] Identification of Legionella pneumophila subspecies in clinical environmental isolates in Japan using the microplate DNA-DNA hybridization method. (2013)
  • Author(s): Junko Amemura-Maekawa, Michiko Koyano, Toshio Yamazaki, Miyo Murai, Makoto Ohnishi, and Fumiaki Kura
  • Organizer: The 8th international conference on Legionella
  • Place of Presentation: Melbourne Australia
• [Presentation] Grouping of clinical isolates of Legionella pneumophila SG1 in Japan, using SBT analysis and environmental habitats (2012)
  • Author(s): Junko Amemura-Maekawa, Fumiaki Kura, Hiroyuki Tawara, Yuko Watanabe, Junko Isobe, Shinobu Tanaka, Hiroshi Nakajima Shuji Yoshino, Shinjiro Abe, Takako Misaki, Tomoe Shimada, Taku Wakui, Yuki Tada, Makoto Ohnishi
  • Organizer: ESGLI 2012 (1st Meeting of the ESCMID Study Group for Legionella Infections)
  • Place of Presentation: Dresden, Germany
• [Presentation] Analysis of Legionella pneumophila serogroup 1 isolates from bath water, cooling tower water, and soil in Japan. (2011)
  • Author(s): Junko Amemura-Maekawa, Kiyomi Kikukawa, J. H. Helbig, Katsunori Furuhata, Masayuki Ichinose, Atsuko Suzuki-Hashimoto, Bin Chang, Miyo Murai, and Fumiaki Kura
  • Organizer: IUMS
  • Place of Presentation: 札幌
• [Presentation] Analysis of Legionella pneumophila serogroup 1 from patients in Japan. (2011)
  • Author(s): Fumiaki Kura, Junko Amemura-Maekawa, Akiko Kaneko, Toshiro Kuroki, Junko Isobe, Masafumi Nukina, Hiroshi Nakajima, Kimiko Kawano, Yuki Tada, and J. H. Helbig
  • Organizer: IUMS
  • Place of Presentation: 札幌
• [Remarks] 前川純子、倉文明、渡辺ユウ、渡辺祐子、磯部順子、田中忍、中嶋洋吉野修司.レジオネラ臨床分離株の型別—レファレンスセンター活動報告としてIASR, 2013, 6:161-3
  • URL: http://www.nih.go.jp/niid/ja/iasr-sp/2252/400/3599-dj4004.html
• [Remarks] レジオネラ臨床分離株の型別－レファレンスセンター活動報告として
  • URL: http://www.nih.go.jp/niid/ja/iasr-sp/2252-related-articles/related-articles-400/3599-dj4004.html