Analysis for role of bacterial cytoskeleton RodZ on virulence gene expression of Shigella type III secretion system

• Project/Area Number: 23590531
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MITOBE Jiro 国立感染症研究所, 細菌第一部, 主任研究官 (40333364)
• Co-Investigator(Kenkyū-buntansha): YANAGIHARA Itaru 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), 免疫部門, 部長 (60314415)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 病原性 / バクテリア細胞骨格 / 転写後制御 / RNA結合蛋白 / III型分泌装置 / 赤痢菌 / mRNA局在 / 転写後調節 / 構造解析 / 細胞骨格

Research Abstract

Bacterial cytoskeleton RodZ was isolated as a factor affecting virulence gene expression of Shigella sonnei. Messenger RNA of the virulence gene was highly stabilized in the deletion mutant of rodZ gene and consistently purified RodZ protein bound to the RNA probe. Finding of amino-acids cluster essential for RNA binding indicated that RodZ has two distinct functions for RNA binding and cell-shape formation. We also found the membrane protein RodZ existed as homohexamer similar to another RNA binding protein Hfq.

Research Products

• [Journal Article] 病原大腸菌のゲノム進化と二成分 (2013)
  • Author(s): 大西真三, 戸部治郎
  • Journal Title: 制御系化学療法の領域 Volume: 29巻 Pages: 53-61
  • URL: https://www.iyaku-j.com/index.php?main_page=index&cPath=5_1_16_3893
• [Journal Article] 病原大腸菌のゲノム進化と二成分制御系 (2012)
  • Author(s): 大西真 三戸部治郎
  • Journal Title: 化学療法の領域 Volume: 38 Pages: 53-61
• [Journal Article] RodZ regulates the post-transcriptional processing of the Shigella sonnei type III secretion system (2011)
  • Author(s): Mitobe J, Yanagihara I, Ohnishi K, Yamamoto S, Ohnishi M, Ishihama A and Watanabe H
  • Journal Title: EMBO report Volume: vol.12(9) Issue: 9 Pages: 911-916
  • DOI: 10.1038/embor.2011.132
  • Peer Reviewed
• [Journal Article] RodZ regulates the post-transcriptional processing of the Shigella sonnei type III secretion system. (2011)
  • Author(s): Mitobe J, Yanagihara I, Ohnishi K, Yamamoto S, Ohnishi M, Ishihama A and Watanabe H
  • Journal Title: EMBO reports Volume: 12(9) Pages: 911-916
  • Peer Reviewed
• [Presentation] 赤痢菌の病原性発現に関わるバクテリア細胞骨格蛋白RodZの多量体形成機構の解析 (2014)
  • Author(s): 三戸部治郎、山本章治、大西真
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀
• [Presentation] 赤痢菌の病原性発現に関わるバクテリア細胞骨格RodZの多量体形成機構の解析 (2014)
  • Author(s): 21. 三戸部治郎 山本章二 大西真
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀
• [Presentation] 赤痢菌Type III secretion systemの発現に関わる細胞骨格蛋白RodZのRNA結合活性と多量体形成機構の解析 (2013)
  • Author(s): 三戸部治郎、山本章治、渡邊治雄、大西真
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ
• [Presentation] Hexamer formation and RNA binding activity of bacterial cytoskeleton RodZ (2013)
  • Author(s): Mitobe J, Yanagihara I, Yamamoto S
  • Organizer: 3rd Conference on Regulating with RNA in Bacteria
  • Place of Presentation: Wurzburg Germany
• [Presentation] Hexamer formation and RNA binding activity of bacteria cytoskeleton RodZ. (2013)
  • Author(s): Mitobe J, Yamamoto S and Yanagihara I
  • Organizer: 3rd Conference on Regulating with RNA in Bacteria
  • Place of Presentation: ドイツ・ヴュルツブルク
• [Presentation] Bacterial cytoskeleton RodZ and virulence gene expression of Shigella type III secretion system (2012)
  • Author(s): Mitobe J, Yamamoto S, Watanabe H, and Ohnishi M
  • Organizer: 第85回日本細菌学会総会ワークショップ細菌における遺伝子発現制御の普遍性と特異性
  • Place of Presentation: 長崎ブリックホール
  • Year and Date: 2012-03-27
• [Presentation] Multimer formation of bacterial cytoskeletal protein RodZ (2012)
  • Author(s): Mitobe J, Yanagihara I, Ohnishi K, Yamamoto S, Watanabe H and Ohnishi M
  • Organizer: 47 th Conference. Cholera and Other Bacterial Enteric Infections
  • Place of Presentation: Chiba Univ. Chiba Japan(US-Japan Cooperative Medical Science Program)
• [Presentation] Multimer formation of bacteria cytoskeletal protein RodZ. (2012)
  • Author(s): Mitobe J, Yamamoto S, Yanagihara I, Ohnishi M , Watanabe H.
  • Organizer: US-Japan Cooperative Medical Science Program 47th Annual Joint Panel Meeting in Chiba on Cholera & other Bacterial Enteric Infections, 2012
  • Place of Presentation: 千葉大学
• [Presentation] RodZ regulates the post-transcriptional processing of the Shigella sonnei type III secretion system 2011 (2011)
  • Author(s): Mitobe J, Yanagihara I, Ohnishi K, Yamamoto S, Ohnishi M, Ishihama A and Watanabe H
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo Convention Center. Sapporo, Japan
• [Presentation] An attempt to develop experimental Shigella vaccine based on virulence gene expression (2011)
  • Author(s): Mitobe J, Koley H, Sinha R, Shimuta K, Koizumi N, Terajima J, Ohnishi M, Nail GB, Watanabe H
  • Organizer: 46th Annual Joint Panel Meeting for Cholera and Other Bacterial Enteric Infections
  • Place of Presentation: Kolkata. India(US-Japan Cooperative Medical Science Program)
• [Presentation] Bacterial cytoskeleton protein RodZ and virulence gene expression of Shigella type III secretion system.
  • Author(s): Jiro Mitobe, Shouji Yamamoto, Makoto Ohnishi, and Haruo Watanabe
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール