Molecular mechanism of the paramyxovirus-mediated membrane fusion as analyzed by novel procedures for detection
Project/Area Number |
23590538
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | Mie University |
Principal Investigator |
TSURUDOME Masato 三重大学, 医学(系)研究科(研究院), 准教授 (50159042)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | パラインフルエンザウイルス / HN蛋白 / F蛋白 / 分子間相互作用 / 膜融合 / パラミクソウイルス / HN蛋白 / 蛋白間相互作用 / PIV5 / 細胞融合 / 多核巨細胞 / 二分子蛍光相補性 / PIV5 / 受容体 |
Research Abstract |
Chimeric analysis of the F proteins of the parainfluenza virus 5 and simian virus 41 revealed that 21 amino acids in the five domains on the surface of the F protein head region are involved in functional interaction with the HN protein stalk region, leading to the induction of cell-cell fusion. Furthermore, it was suggested that this functional interaction is dependent on the conformation of the HN stalk region. On the other hand, the results obtained from bimolecular fluorescence complementation analysis indicated that physical interaction between HN and F protein is not only dependent on the proteolytic cleavage of the F protein but also on the conformational change of the F protein. However, it was suggested that under physiological conditions his physical interaction is weak in its avidity or takes place very transiently.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Calcium ionophore A23187 inhibits human parainfluenza virus type 2 growth and monoclonal antibody against CD98 heavy chain recovers the inhibition2014
Author(s)
Kihira S,Uematsu J,Ishitana Y,Chindoh M,Baba A,Kuzuta R,Hasegawa T,Fujimoto K,Funauchi A,Yamamoto H,Kawano M,Tsurudome M,O'Brien M,Komada H
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Journal Title
Int. J. Sci
Volume: 3
Pages: 14-20
Related Report
Peer Reviewed
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[Journal Article] Calcium ionophore A23187 inhibits human parainfluenza virus type 2 growth and monoclonal antibody against CD98 heavy chain recovers the inhibition2014
Author(s)
Kihira S, Uematsu J,Ishitana Y, Chindoh M, Baba A, Kuzuta R, Hasegawa T, Fujimoto K, Funauchi A, Yamamoto H, Kawano M, Tsurudome M, O'Brien M, Komada H.
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Journal Title
International Journal of Sciences
Volume: 3
Pages: 14-20
Related Report
Peer Reviewed
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[Journal Article] Intranasally administered antigen 85B gene vaccine in non-replicating human Parainfluenza type 2 virus vector ameliorates mouse atopic dermatitis2013
Author(s)
Kitagawa H,Kawano M,Yamanaka K,Kakeda M,Tsuda K,Inada H,Yoneda M,Sakaguchi T,Nigi A,Nishimura K,Komada H,Tsurudome M,Yasutomi Y,Nosaka T,Mizutani H
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Peer Reviewed
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[Journal Article] Intranasally administered antigen 85B gene vaccine in non-replicating:human parainfluenza type 2 virus vector ameliorates mouse atopic dermatitis2013
Author(s)
Kitagawa H, Kawano M, Yamanaka K, Kakeda M, Tsuda K, Inada H, Yoneda M, Sakaguchi T, Nigi A, Nishimura K, Komada H, Tsurudome M, Yasutomi Y, Nosaka T, Mizutani H
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Peer Reviewed
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[Journal Article] Legume lectins inhibit human parainfluenza virus type 2 infection by interfering with the entry2012
Author(s)
Uematsu J,Koyama A,Takano S,Ura Y,Tanemura M,Kihira S,Yamamoto H,Kawano M,Tsurudome M,O'Brien M,Komada H
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Journal Title
Viruses
Volume: 4
Pages: 1104-1115
Related Report
Peer Reviewed
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[Journal Article] Legume lectins inhibit human parainfluenza:virus type 2 infection by interfering with the entry.2012
Author(s)
Uematsu J, Koyama A, Takano S, Ura Y, Tanemura M, Kihira S, Yamamoto H, Kawano M, Tsurudome M, O'Brien M, Komada H.
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Journal Title
Viruses-Basel
Volume: 4
Issue: 7
Pages: 1114-1115
DOI
Related Report
Peer Reviewed
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[Journal Article] Completion of the full-length genome sequence of human parainfluenza virus types 4A and 4B : sequence analysis of the large protein genes and gene start,intergenic and end sequences2011
Author(s)
Komada H,Kawano M,Uefuji A,Ito M,TsurudomeM,Hatakeyama E,Nakanishi M,Sakue S,Joh C,Suzumura E,Tamaki T,Tomioka T,Nishio M,Tsumura H,Uematsu J,Yamamoto H,O'Brien M,Bando H,Ito
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Journal Title
Arch. Virol
Volume: 156
Pages: 161-166
Related Report
Peer Reviewed
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[Presentation]2012
Author(s)
山本秀孝,紀平佐保子,植松淳,河野光雄,鶴留雅人,駒田洋
Organizer
日本社会薬学会第31年会
Place of Presentation
鈴鹿
Year and Date
2012-09-16
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