Analysis of virus replication mechanism using minigenome or recombinant virus system

• Project/Area Number: 23590539
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Wakayama Medical University
• Principal Investigator: NISHIO Machiko 和歌山県立医科大学, 医学部, 教授 (70156040)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: パラインフルエンザウイルス２型 / リコンビナントウイルス / ミニゲノムシステム / V蛋白 / ウイルス増殖機構 / caspase-1 / tetherin / 増殖機構 / パラミクソウイルス / ウイルス増殖 / ミニゲノム

Research Abstract

To search for host factors interacting with human parainfluenza virus type 2 (hPIV2) V protein systematically, I used the yeast two-hybrid system, and identified some proteins. Tetherin is a cellular membrane protein that restricts budding of a number of enveloped viruses. I demonstrated that tetherin interacts with V protein, and inhibits hPIV2 growth. The V protein inhibits the caspase-1-mediated maturation of IL-1beta via its interaction with caspase-1. Moreover, it interacts with adaptor-related protein complex 1 (AP-1) that is important for apical sorting of hPIV2.

Research Products

• [Journal Article] Full conversion of the hemagglutinin-neuraminidase specificity of the parainfluenza virus 5 fusion protein by replacement of 21 amino acids in its head region with those of the simian virus 41 fusion protein (2013)
  • Author(s): Tsurudome M, Nakahashi M, Matsushima Y, Ito M, Nishio M, Kawano M, Komada H, Nosaka T
  • Journal Title: J . Virol Volume: 87 Pages: 8342-8350
  • Peer Reviewed
• [Journal Article] Human parainfluenza virus type 2 V Protein inhibits TRAF6-mediated ubiquitination of IRF7 to prevent TLR7- and TLR9-dependent interferon induction (2013)
  • Author(s): Kitagawa Y, Yamaguchi M, Zhou M, Nishio M, Itoh M, Gotoh B
  • Journal Title: J . Virol Volume: 87 Pages: 7966-7976
  • Peer Reviewed
• [Journal Article] Full conversion of the hemagglutinin-neuraminidase specificity of the parainfluenza virus 5 fusion protein by replacement of 21 amino acids in its head region with those of the simian virus 41 fusion protein. (2013)
  • Author(s): Tsurudome M, Nakahashi M, Matsushima Y, Ito M, Nishio M, Kawano M, Komada H, Nosaka T.
  • Journal Title: J. Virol. Volume: 87 Issue: 15 Pages: 8342-8350
  • DOI: 10.1128/jvi.03549-12
  • Peer Reviewed
• [Journal Article] Human Parainfluenza Virus Type 2 V Protein Inhibits TRAF6-Mediated Ubiquitination of IRF7 To Prevent TLR7- and TLR9-Dependent Interferon Induction. (2013)
  • Author(s): Kitagawa Y, Yamaguchi M, Zhou M, Nishio M, Itoh M, Gotoh B.
  • Journal Title: J. Virol. Volume: 87 Issue: 14 Pages: 7966-7976
  • DOI: 10.1128/jvi.03525-12
  • Peer Reviewed
• [Journal Article] A tryptophan-rich motif in the human parainfluenza virus type 2 V protein is critical for the blockade of toll-like receptor 7 (TLR7)- and TLR9-dependent signaling (2011)
  • Author(s): Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Takeuchi K, Itoh M, Gotoh B
  • Journal Title: J . Virol Volume: 85 Pages: 4606-4611
  • Peer Reviewed
• [Journal Article] Identification of domains on the fusion (F) protein trimer that influence the hemagglutinin-neuraminidase specificity of the F protein in mediating cell-cell fusion (2011)
  • Author(s): Tsurudome M, Ito M, Nishio M, Nakahashi M, Kawano M, Komada H, Nosaka T, Ito Y
  • Journal Title: J . Virol Volume: 85 Pages: 3153-3161
  • Peer Reviewed
• [Journal Article] Identification of domains on the F protein trimer that influence the hemagglutinin-neuraminidase specificity of the F protein in mediating cell-cell fusion. (2011)
  • Author(s): Tsurudome M, Ito M, Nishio M, Nakahashi M, Kawano M, Komada H, Nosaka T, Ito Y.
  • Journal Title: Journal of Virology Volume: 85 Pages: 3153-3161
  • Peer Reviewed
• [Journal Article] A tryptophan-rich motif in hPIV2 V protein is critical for the blockade of toll-like receptor 7 (TLR7)-and TRL9-dependent signaling. (2011)
  • Author(s): Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Tadeuchi K, Itoh M, Gotoh B.
  • Journal Title: Journal of Virology Volume: 85 Pages: 4606-4611
  • Peer Reviewed
• [Presentation] ヒトパラインフルエンザウイルス2型(hPIV2)V蛋白と結合する宿主細胞蛋白AP1M1及びAP1M2がウイルス増殖に与える影響 (2013)
  • Author(s): 西尾真智子、太田圭介、湯峰奈都子、鶴留雅人、五藤秀男
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] ヒトパラインフルエンザウイルス2型(hPIV2)とTetherin(BST-2)の相互作用 (2013)
  • Author(s): 太田圭介、五藤秀男、湯峰奈都子、西尾真智子
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] ヒトパラインフルエンザウイルス2型の感染によるA型インフルエンザウイルスの増殖促進 (2013)
  • Author(s): 五藤秀男、太田圭介、湯峰奈都子、西尾真智子
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] パラインフルエンザウイルスのHN蛋白とF蛋白の機能的相互作用の解析 (2013)
  • Author(s): 鶴留雅人、伊藤守弘、西尾真智子、河野光雄、駒田洋、大塚順平、野阪哲哉
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Kolakofsky D Human parainfluenza virus type 2 V protein inhibits activity of caspase-1 (2013)
  • Author(s): Nishio M, OhtanK, Tsurudome M
  • Organizer: XV International conference on Negative Strand Viruses
  • Place of Presentation: グラナダ、スペイン
• [Presentation] Interaction between human parainfluenza virus type 2 V protein and tetherin, an antiviral host factor (2013)
  • Author(s): Ohta K, Goto H, Yumine N, Nishio M
  • Organizer: XV International conference on Negative Strand Viruses
  • Place of Presentation: グラナダ、スペイン
• [Presentation] Conversion of the parainfluenza virus 5 F protein to a simian virus 41 HN-specfic protein by amino acid substitutions (2013)
  • Author(s): Tsurudome M, Ito M, Nishio M, Nosaka T
  • Organizer: XV International conference on Negative Strand Viruses
  • Place of Presentation: グラナダ、スペイン
• [Presentation] 貝類から分離したウェルシュ菌の解析 (2013)
  • Author(s): 湯峰奈都子、太田圭介、西尾真智子
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張
• [Presentation] ヒトパラインフルエンザウイルス２型(hPIV2)V蛋白と結合する宿主蛋白AP1M1及びAP1M2がウイルス増殖に与える影響 (2013)
  • Author(s): 西尾真智子、太田圭介、湯峯奈都子、鶴留雅人、五藤秀男
  • Organizer: 第６１回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] パラインフルエンザウイルスのHN蛋白とF蛋白の機能的相互作用の解析 (2013)
  • Author(s): 鶴留雅人、伊藤守弘、西尾真智子、河野光雄、駒田洋、大塚順平、野坂哲哉
  • Organizer: 第６１回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] パラインフルエンザウイルス２型の感染によるA型インフルエンザウイルスの増殖促進 (2013)
  • Author(s): 五藤秀男、太田圭介、湯峯奈都子、西尾真智子
  • Organizer: 第６１回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] パラインフルエンザウイルス２型(hPIV-2)とTetherin(BST-2)の相互作用 (2013)
  • Author(s): 太田圭介、五藤秀男、湯峯奈都子、西尾真智子
  • Organizer: 第６１回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 貝類から分離したウェルシュ菌の解析 (2013)
  • Author(s): 湯峰奈都子、太田圭介、西尾真智子
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] ヒトパラインフルエンザウイルス2型(hPIV2)V蛋白によるcaspase-1活性化抑制 (2012)
  • Author(s): 西尾真智子、太田圭介、湯峰奈都子、五藤秀男
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] TLR7/9依存性インターフェロン産生抑制におけるV蛋白質とTRF6の相互作用の重要性 (2012)
  • Author(s): 北川善紀、山口まゆ、小松孝行、周敏、竹内健司、西尾真智子、伊藤正恵、後藤敏
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 分子学的手法を用いた食肉中の腸管病原性C.perfringensの検出 (2012)
  • Author(s): 金子郁子、宮本和明、三木康弘、湯峯奈都子、西尾真智子、秋本茂
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] ヒトパラインフルエンザウイルス2型（hPIV2）V蛋白によるcaspase-1活性化抑制 (2012)
  • Author(s): 西尾真智子、太田圭介、湯峰奈都子、五藤秀男
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Nucleocytoplasmic shuttling of the human parainfluenza virus type 2 P protein (2011)
  • Author(s): Nishio M, Ohtsuka J, Tsurudome M, Nosaka T, Kolakofsky D
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] Identification of amino acids of simian virus 41 (SV41) fusion protein that convert parainfluenza virus 5 fusion protein to a protein which specifically interacts with SV41 hemagglutinin-neuraminidase by substitution (2011)
  • Author(s): Tsurudome M, Nakahashi M, Matsushima Y, Nishio M, Kawano M, Nosaka T
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] Antagonistic activity of paramyxovirus V proteins against toll-like receptor 7/9 dependent alpha interferon induction (2011)
  • Author(s): Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Takeuchi K, Itoh M, Gotoh B
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] Identification of amino acids of simian virus 41 (SV41) fusion protein that convert parainfluenza virus 5 fusion protein to a protein which specifically interacts with SV41 hemagglutinin-neuraminidase by substitution. (2011)
  • Author(s): Tsurudome M, Nakahashi M, Matsushima Y, Nishio M, Kawano M, Komada H, Nosaka T.
  • Organizer: IUMS2011
  • Place of Presentation: 札幌
• [Presentation] Antagonistic activity of paramyxovirus V proteins against toll-like receptor 7/9 dependent alpha interferon induction. (2011)
  • Author(s): Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Tadeuchi K, Itoh M, Gotoh B.
  • Organizer: IUMS2011
  • Place of Presentation: 札幌
• [Presentation] Nucleocytoplasmic shuttling of human parainfluenza virus type 2 P protein. (2011)
  • Author(s): Nishio M, Ohtsuka j, tsurudome M, Nosaka T, Kolakofsky D.
  • Organizer: IUMS2011
  • Place of Presentation: 札幌