Analysis of Restriction Factors of cynomolgus monkey in establishment of HIV-1/monkey model

• Project/Area Number: 23590541
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: NAKAYAMA Emi 大阪大学, 微生物病研究所, 准教授 (70324845)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ヒト免疫不全ウイルス / 感染抑制因子 / 動物モデル / HIV-1感染 / TRIM5α / TRIMCyp / TRIM5 / cynomolgus monkey / HIV-1

Research Abstract

TRIM5alpha was previously identified as an intrinsic factor that restricts HIV-1 replication in rhesus monkey cells. Thereafter, TRIMCyp was discovered as a mutated allele of TRIM5 gene. We demonstrated that the frequencies of TRIMCyp in cynomolgus monkey markedly higher than that of rhesus monkey. Then, we infected monkey-tropic HIV-1 (MN4Rh-3) to TRIMCyp homozygotes or TRIM5alpha homozygotes. The viral growth was higher in TRIMCyp homozygotes than that in TRIM5alpha homozygotes. These results indicated that genotyping of TRIM5 gene is important to select monkey susceptible to monkey-tropic HIV-1 in establishment of HIV-1/monkey model system.

Research Products

• [Journal Article] Moderate Restriction of Macrophage-Tropic Human Immunodeficiency Virus Type 1 by SAMHD1 in Monocyte-Derived Macrophages (2014)
  • Author(s): Taya K, Nakayama EE, Shioda T
  • Journal Title: PLoS One Volume: 9(3) Issue: 3 Pages: e90969-e90969
  • DOI: 10.1371/journal.pone.0090969
  • Peer Reviewed
• [Journal Article] Generation of rhesus macaque-tropic HIV-1 clones that are resistant to major anti-HIV-1 restriction factors (2013)
  • Author(s): Nomaguchi M, Yokoyama M, Kono K, Nakayama EE, Shioda T, Doi N, Fujiwara S, Saito A, Akari H, Miyakawa K, Ryo A, Ode H, Iwatani Y, Miura T, Igarashi T, Sato H, Adachi A
  • Journal Title: J Virol Volume: 87(21) Issue: 21 Pages: 11447-11461
  • DOI: 10.1128/jvi.01549-13
  • Peer Reviewed
• [Journal Article] Slower Uncoating Is Associated with Impaired Replicative Capability of Simian-Tropic HIV-1 (2013)
  • Author(s): Kono K, Takeda E, Tsutsui H, Kuroishi A, Hulme AE, Hope TJ, Nakayama EE, Shioda T
  • Journal Title: PLoS One Volume: 8(8) Issue: 8 Pages: e72531-e72531
  • DOI: 10.1371/journal.pone.0072531
  • Peer Reviewed
• [Journal Article] TRIM5 genotypes in cynomolgus monkeys primarily influence inter-individual diversity in susceptibility to monkey-tropic human immunodeficiency virus type 1 (2013)
  • Author(s): Saito A, Nomaguchi M, Kono K, Iwatani Y, Yokoyama M, Yasutomi Y, Sato H, Shioda T, Sugiura W, Matano T, Adachi A, Nakayama E, Akari H
  • Journal Title: J Gen Virol Volume: 94(Pt 6) Issue: 6 Pages: 1318-24
  • DOI: 10.1099/vir.0.050252-0
  • Peer Reviewed
• [Journal Article] A naturally occurring single amino acid substitution in human TRIM5α linker region affects its anti-HIV-1 activity and susceptibility to HIV-1 infection (2013)
  • Author(s): Nakayama EE, Nakajima T, Kaur G, Mimaya JI, Terunuma H, Mehra N, Kimura A, Shioda T
  • Journal Title: AIDS Res Hum Retroviruses Volume: 29(6) Issue: 6 Pages: 919-24
  • DOI: 10.1089/aid.2012.0369
  • Peer Reviewed
• [Journal Article] Systemic biological analysis of the mutations in two distinct HIV-1mt genomes occurred during replication in macaque cells (2013)
  • Author(s): Nomaguchi M, DOI N, Fujiwara S, Saito A, Akari H, Nakayama EE, Shioda T, Yokoyama M, Sato H, Adachi A
  • Journal Title: Microbes Infect Volume: 15(4) Issue: 4 Pages: 319-28
  • DOI: 10.1016/j.micinf.2013.01.005
  • Peer Reviewed
• [Journal Article] Gag-CA Q110D mutation elicits TRIM5-independent enhancement of HIV-1mt replication in macaque cells (2013)
  • Author(s): Nomaguchi M, Yokoyama M, Kono K, Nakayama EE, Shioda T, Saito A, Akari H, Yasutomi Y, Matano T, Sato H, Adachi A
  • Journal Title: Microbes and Infection Volume: 15(1) Issue: 1 Pages: 56-65
  • DOI: 10.1016/j.micinf.2012.10.013
  • Peer Reviewed
• [Journal Article] Polymorphisms in Fas gene is associated with HIV-related lipoatrophy in Thai patients (2013)
  • Author(s): Likanonsakul S, Rattanatham T, Feangvad S, Uttayamakul S, Prasithsirikul W, Srisopha S, Nitiyanontakij R, Tengtrakulcharoen P, Tarkowski M, Riva A, Nakayama EE, Shioda T
  • Journal Title: AIDS Res Hum Retroviruses Volume: 29(1) Issue: 1 Pages: 142-50
  • DOI: 10.1089/aid.2012.0114
  • Peer Reviewed
• [Journal Article] TRIM5 genotypes in cynomolgus monkeys primarily influence inter-individual diversity in susceptibility to monkey-tropic human immunodeficiency virus type 1. (2013)
  • Author(s): Saito A, Nomaguchi M, Kono K, Iwatani Y, Yokoyama M, Yasutomi Y, Sato H, Shioda T, Sugiura W, Matano T, Adachi A, Nakayama EE, Akari H.
  • Journal Title: Journal of General Virology Volume: 未定
  • Peer Reviewed
• [Journal Article] A naturally occurring single amino acid substitution in human TRIM5a linker region affects its anti-HIV-1 activity and susceptibility to HIV-1 infection. (2013)
  • Author(s): Nakayama EE, Nakajima T, Kaur G, Mimaya J, Terunuma H, Mehra N, Kimura A, Shioda T.
  • Journal Title: AIDS Research and Humanretroviruses Volume: 未定
  • Peer Reviewed
• [Journal Article] The carboxyl-terminus of human immunodeficiency virus type 2 circulating recombinant form 01_AB capsid protein affects sensitivity to human TRIM5 α (2012)
  • Author(s): Miyamoto T, Nakayama EE, Yokoyama M, Ibe S, Takehara S, Kono K, Yokomaku Y, Pizzato M, Luban J, Sugiura W, Sato H, Shioda T
  • Journal Title: PLoS One Volume: 7(10) Issue: 10 Pages: 347757-347757
  • DOI: 10.1371/journal.pone.0047757
  • Peer Reviewed
• [Journal Article] Allele Frequency of Antiretroviral Host Factor TRIMCyp in Wild-caught Cynomolgus Macaques (Macaca fascicularis) (2012)
  • Author(s): Saito A, Kawamoto Y, Higashino A, Yoshida T, Ikoma T, Suzaki Y, Ami Y, Shioda T, Nakayama EE and Akari H
  • Journal Title: Front Microbio Volume: 3 Pages: 314-314
  • Peer Reviewed
• [Journal Article] Elicitation of Both Anti HIV-1 Env Humoral and Cellular Immunities by Replicating Vaccinia Prime Sendai Virus Boost Regimen and Boosting by CD40Lm (2012)
  • Author(s): Zhang X, Sobue T, Isshiki M, Makino S, Inoue M, Kato K, Shioda T, Ohashi T, Sato H, Komano J, Hanabusa H, Shida H
  • Journal Title: PLoS One Volume: 7(12) Pages: 206-206
  • DOI: 10.3389/fmicb.2012.00206
  • Peer Reviewed
• [Journal Article] Role of human TRIM5αin intrinsic inmunity (2012)
  • Author(s): Nakayama EE
  • Journal Title: Front Microbiol Volume: 3 Pages: 97-97
  • DOI: 10.3389/fmicb.2012.00097
  • Peer Reviewed
• [Journal Article] TRIM5αand species tropism of HIV/SIV (2012)
  • Author(s): Nakayama EE, Shioda T
  • Journal Title: Front Microbiol Volume: 3(eCollection 2012) Pages: 13-13
  • Peer Reviewed
• [Journal Article] Geographical, genetic and functional diversity of antiretroviral host factor TRIMCyp in cynomolgus macaque (Macaca fascicularis) (2012)
  • Author(s): Akatsuki Saito
  • Journal Title: Journal of General Virology Volume: 93 Issue: 3 Pages: 594-602
  • DOI: 10.1099/vir.0.038075-0
  • Peer Reviewed
• [Journal Article] Allele frequency of antiretroviral host factor TRIMCyp in wild-caught cynomolgus macaques (Macaca fascicularis) (2012)
  • Author(s): Saito A、Kawamoto Y、Higashino A、Yoshida T、Ikoma T、Suzaki Y、Ami Y、Shioda T、Nakayama EE、Akari H
  • Journal Title: Frontier in Microbiology Volume: Vol 3 Pages: 314-314
  • DOI: 10.3389/fmicb.2012.00314
  • Peer Reviewed
• [Journal Article] Geographic, Genetic and functional diversity of antiretroviral host factor TRIMCyp in cynomolgus macaque (Macaca fascicularis). (2012)
  • Author(s): Saito A, Kono K, Nomaguchi M, Yasutomi Y, Adachi A, Shioda T, Akari H, Nakayama EE
  • Journal Title: Journal of General Virology Volume: 93 Pages: 594-602
  • Peer Reviewed
• [Journal Article] TRIM5 α and Species Tropism of HIV/SIV (2012)
  • Author(s): Nakayama EE
  • Journal Title: Front Microbiol Volume: 3 Pages: 13-13
  • DOI: 10.3389/fmicb.2012.00013
  • Peer Reviewed
• [Journal Article] A Single Amino Acid of Human Immunodeficiency Virus Type 2 Capsid Protein Affects Conformation of Two External Loops and Viral Sensitivity to TRIM5alpha (2011)
  • Author(s): Miyamoto T, Yokoyama M, Kono K, Shioda T, Sato H, Nakayama EE
  • Journal Title: PLoS ONE Volume: 6(7) Issue: 7 Pages: e22779-e22779
  • DOI: 10.1371/journal.pone.0022779
  • Peer Reviewed
• [Journal Article] A novel monkey-tropic HIV-1 derivative encoding only minimal SIV sequences can replicate in cynomolgus monkeys. (2011)
  • Author(s): Saito A, Nomaguchi M, Iijima S, Kuroishi A, Yoshida T, Lee Y, Hayakawa T, Kono K, Nakayama EE, Shioda T, Yasutomi Y, Adachi A, Matano T, Akari H
  • Journal Title: Microbes and Infection Volume: 13 Pages: 58-64
  • Peer Reviewed
• [Journal Article] A Single Amino Acid of Human Immunodeficiency Virus Type 2 Capsid Protein Affects Conformation of Two External Loops and Viral Sensitivity to TRIM5alpha. (2011)
  • Author(s): Miyamoto T, Yokoyama M, Kono K, Shioda T, Sato H, Nakayama EE
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Presentation] マクロファージ指向性HIV-1も、マクロファージおよび単球においてSAMHD1による増殖抑制を受けている (2013)
  • Author(s): 田谷かほる
  • Organizer: 第27回日本エイズ学会学術集会・総会
  • Place of Presentation: 熊本
• [Presentation] 可視化ウイルスをつかったHIV-2カプシドコアの脱核速度測定法の確立 (2013)
  • Author(s): 武田英里
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Diversity of antiretroviral host factor TRIM5 gene in macaque monkeys (2013)
  • Author(s): Akatsuki Saito
  • Organizer: Retroviruses 2013
  • Place of Presentation: Cold Sprinig Harbor, NY
• [Presentation] Slower uncoating has a deleterious effect on replication of the simian-tropic HIV-1 (2013)
  • Author(s): Emi E. Nakayama
  • Organizer: Retroviruses 2013
  • Place of Presentation: Cold Sprinig Harbor, NY
• [Presentation] Diversity of antiretroviral host factor TRIM5 gene in macaque monkeys. (2013)
  • Author(s): Akatsuki Saito, Emi E. Nakayama, Tatuo Shioda, Tomoyuki Yoshida, Atsunori Higashio, Saori Suzuki, Yoshi Kawaoka, Hirofumi Akari.
  • Organizer: Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] Slower uncoating has a deleterious effect on replication of the simian-tropic HIV-1. (2013)
  • Author(s): Ken Kono, Eri Takeda, Hiromi Tsutui, Ayumu Kuroishi, Amy E. Hulme, Thomas J. Hope, Emi E. Nakayama, Tatsuo Shioda.
  • Organizer: Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] マクロファージ指向性 HIV-1も、マクロファージおよび単球において SAMHD1 による増殖抑制を受けている. (2013)
  • Author(s): 田谷かほる、中山英美、塩田達雄
  • Organizer: 第27回日本エイズ学会学術集会・総会
  • Place of Presentation: 熊本
• [Presentation] ヒトTRIM5αリンカー領域の多型の抗HIV-1活性に及ぼす影響 (2012)
  • Author(s): 中山英美
  • Organizer: 第26回エイズ学会学術集会・総会
  • Place of Presentation: 横浜
• [Presentation] A Naturally Occurring Single Amino Acid Substitution in Human TRIM5αLinker Region Affects Its Anti-HIV-1 Activity and Susceptibility to HIV-1 Infection (2012)
  • Author(s): Emi E. Nakayama
  • Organizer: Retroviruses 2012
  • Place of Presentation: NY(USA)
• [Presentation] Genetic Diversity of TRIM5 Gene and HIV-1 Susceptibility in Cynomolgus Magaque(Macaca Fascicularis) (2012)
  • Author(s): Emi E. Nakayama
  • Organizer: Retroviruses 2012
  • Place of Presentation: NY(USA)
• [Presentation] A Naturally Occurring Single Amino Acid Substitution in Human TRIM5α Linker Region Affects Its Anti-HIV-1 Activity and Susceptibility to HIV-1 Infection. (2012)
  • Author(s): E Nakayama, T Nakajima, G Kaur, H Terunuma, J-I Mimaya, H Ohtani, N Mehra, A Kimura, T Shioda
  • Organizer: Cold Spring Harbor Laboratory (Retroviruses)2012
  • Place of Presentation: NY
• [Presentation] Genetic Diversity of TRIM5 Gene and HIV-1 Susceptibility in Cynomolgus Macaque (Macaca fascicularis) (2012)
  • Author(s): Akatsuki Saito, Ken Kono, Masako Nomaguchi, Masaru Yokoyama, Yasuhiro Yasutomi, Tetsuro Matano, Hironori Sato, Tatsuo Shioda, Akio Adachi, Hirofumi Akari, Emi E Nakayama
  • Organizer: Cold Spring Harbor Laboratory (Retroviruses)2012
  • Place of Presentation: NY
• [Presentation] ヒトTRIM5α リンカー領域の多型の抗HIV-1活性に及ぼす影響 (2012)
  • Author(s): 中山英美、中島敏晶、Gurvinder Kaur、三間屋純一、照沼 裕、Narinder Mehra、木村彰方、塩田達雄
  • Organizer: 第26回エイズ学会学術集会・総会
  • Place of Presentation: 横浜
• [Presentation] 抗レトロウイルス療法副作用の発症に関わる宿主因子 (2011)
  • Author(s): 中山英美
  • Organizer: 第25回エイズ学会学術集会・総会
  • Place of Presentation: 東京
• [Presentation] サル指向性HIV-1への感受性に影響を与えるマカクサルTRIM5遺伝子の多様性 (2011)
  • Author(s): 齋藤暁
  • Organizer: 第25回エイズ学会学術集会・総会
  • Place of Presentation: 東京
• [Presentation] (2011)
  • Author(s): Saito A
  • Organizer: 第59回日本ウイルス学会学術集会(International Congress of Virology)
  • Place of Presentation: 札幌
• [Presentation] A single amino acid of human immunodeficiency virus type 2 capsid protein affects conformation of two external loops and viral sensitivity to TRIM5α (2011)
  • Author(s): Miyamoto T
  • Organizer: 第59回日本ウイルス学会学術集会(International Congress of Virology)
  • Place of Presentation: 札幌
• [Presentation] Simian-tropic HIV-1 NL-4/5S6/7SVifS shows slower capsid uncoating in human cells (2011)
  • Author(s): Kono K.
  • Organizer: 第59回日本ウイルス学会学術集会(International Congress of Virology)
  • Place of Presentation: 札幌
• [Presentation] サル指向性HIV-1への感受性に影響を与えるマカクサルTRIM5遺伝子の多様性. (2011)
  • Author(s): 齋藤暁、河野健、中山英美、足立昭夫、野間口雅子、保富康宏、俣野哲朗、塩田達雄、明里宏文
  • Organizer: 第25回エイズ学会学術集会・総会
  • Place of Presentation: 東京
• [Presentation] 宿主遺伝子多型とHIV感染症 (2011)
  • Author(s): 中山英美
  • Organizer: 第25回エイズ学会学術集会・総会(招待講演)
  • Place of Presentation: 東京