Development of murine model for dengue virus infection
| Project/Area Number |
23590542
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
KUROSU TAKESHI 大阪大学, 微生物病研究所, 招へい教員 (70432432)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | デングウイルス / 熱帯感染症 / 蚊媒介性疾患 / 新興・再興感染症 / ウイルス / 感染症 / 動物モデル / 出血熱 / 血漿漏出 / マウスモデル / フラビウイルス / 振興・再興感染症 |
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| Outline of Final Research Achievements |
The mechanism of severe dengue remains unknown, and there is no effective drug or therapy against dengue virus infection. To understand the mechanism of severe dengue, we established the mouse model system using the recombinant chimeric dengue virus and interferon knock out mice. Mice infected with the chimeric dengue virus showed plasma leakage, thrombocytopenia and antibody-dependent enhancement which have been seen in patients with severe dengue. Tumor necrosis factor (TNF) alpha was a critical factor for severe diseases. In addition, we observed strong bone marrow suppression, which is related to thrombocytopenia.
We examined if this model could be applied to study the therapeutic effect and side effect of anti-dengue virus antibody. This model is a valid model system to study pathogenesis of severe dengue and development of therapeutics.
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Report
(5 results)
Research Products
(25 results)
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[Journal Article] A highly conserved region between amino acids 221 and 266 of dengue virus non-structural protein 1 is a major epitope region in infected patients.2014
Author(s)
Omokoko MD, Pambudi S, Phanthanawiboon S, Masrinoul P, Setthapramote C, Sasaki T, Kuhara M, Ramasoota P, Yamashita A, Hirai I, Ikuta K, Kurosu T.
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Journal Title
Am J Trop Med Hyg.
Volume: 91
Issue: 1
Pages: 146-155
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Platelet Apoptosis and Apoptotic Platelet Clearance by Macrophages in Secondary Dengue Virus Infections.2012
Author(s)
Alonzo, M., Lacuesta, T., Dimaano, EM., Kurosu, T., Suarez, LC., Mapua, CA Akeda, Y., Matias, RR., Kuter, DJ., Nagata, S., Natividad, FF. and Oishi, K.
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Journal Title
J Infect Dis.
Volume: 205
Issue: 8
Pages: 1321-1329
DOI
Related Report
Peer Reviewed
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