| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The mechanism of severe dengue remains unknown, and there is no effective drug or therapy against dengue virus infection. To understand the mechanism of severe dengue, we established the mouse model system using the recombinant chimeric dengue virus and interferon knock out mice. Mice infected with the chimeric dengue virus showed plasma leakage, thrombocytopenia and antibody-dependent enhancement which have been seen in patients with severe dengue. Tumor necrosis factor (TNF) alpha was a critical factor for severe diseases. In addition, we observed strong bone marrow suppression, which is related to thrombocytopenia.
We examined if this model could be applied to study the therapeutic effect and side effect of anti-dengue virus antibody. This model is a valid model system to study pathogenesis of severe dengue and development of therapeutics.
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