Analysis for the IL28B SNPs and IFN sensitivity using HCV cell culture models
Project/Area Number |
23590544
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | Kagoshima University (2014) Okayama University (2011-2013) |
Principal Investigator |
MASANORI Ikeda 鹿児島大学, 医歯(薬)学総合研究科, 教授 (30315767)
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Project Period (FY) |
2011-04-28 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | HCV / IL28B SNPs / HuH-7 / Li23 / IFN-α / IFN-λ / IL28B / インターフェロン / IL28B SNP / rs8099917 / rs12979860 / rs8113007 / C型肝炎ウイルス |
Outline of Final Research Achievements |
The current standard therapy for chronic hepatitis C is based on pegylated-interferon (PEG-IFN)/ribavirin (RBV). In 2011, HCV protease inhibitor entered the clinical stage. The new treatment with protease inhibitor is also based on a combination of PEG-IFN/RBV. In 2009, SNPs upstream from the IL28B gene has been reported as strong predictive host factors of the effect of PEG-IFN/RBV. However, the precise mechanism underlying this influence is unclear. Therefore, we examined the IL28B genotypes for human hepatocyte cell lines in order to develop a cell culture model. HCV permissive Li23 and HuH-7 cells are exhibited IFN-sensitive and -resistant IL28B SNPs, respectively. The sensitivities to IFN-α are not significantly different in HCV harboring Li23 and HuH-7 cells. However, IFN-λ exhibited 100 times higher inhibitory effect on HCV RNA replication in Li23 cells than in HuH-7 cells. These HCV cell culture systems are useful tools for the study of IL28B SNPs and HCV.
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Report
(5 results)
Research Products
(31 results)
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[Journal Article] Anti-ulcer agent teprenone inhibits hepatitis C virus replication: Potential treatment for hepatitis C.2011
Author(s)
Ikeda M, Kawai Y, Mori K, Yano M, Abe K, Nishimura G, Dansako H, Ariumi Y, Wakita T, Yamamoto K, Kato N.
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Journal Title
Liver Int.
Volume: 31
Pages: 871-880
Related Report
Peer Reviewed
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[Presentation] Characterization of anti-HCV activity of N-251, a preclinical antimalarial drug, and its combination effect with DAA.2014
Author(s)
Ueda Y, Kim HS, Dansako H, Satoh S, Ikeda M, Doi H, Wataya Y, Kato N.
Organizer
21st international symposium on hepatitis C virus and related viruses
Place of Presentation
Banff, Canada
Year and Date
2014-09-07 – 2014-09-11
Related Report
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