• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of human norovirus replication and pathogenicity

Research Project

Project/Area Number 23590556
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

KATAYAMA Kazuhiko  国立感染症研究所, 国立感染症研究所, 室長 (60342903)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords病原性 / 細胞内同多 / 機能 / 細胞内動態
Research Abstract

We established a new reverse genetics system driven by a mammalian promoter, that functions without helper virus. The complete genome of the HuNoV GII.3 U201, Saga1, TCH, NV68 and also MNV-S7 strain was cloned downstream of an EF-1 alpha mammalian promoter. These constructs produced progeny viruses produced from cells that contained the complete NoV genomic RNA, VP1, VP2 and VPg were detected in isopycnic gradients of CsCl at the same density as native infectious NoV particles from a patient's stool. A GFP reporter construct containing the GFP gene in ORF1 produced complete virions that contain VPg linked RNA. RNA from virions containing the encapsidated GFP genomic RNA was successfully transfected back into cells producing fluorescent puncta indicating that the encapsidated RNA is replication competent. Our results are useful to develop of an antiviral medicine which targeted these in future.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (43 results)

All 2014 2013 2012 2011 Other

All Journal Article (16 results) (of which Peer Reviewed: 13 results) Presentation (27 results) (of which Invited: 1 results)

  • [Journal Article] A plasmid based human norovirus reverse genetics system produces reporter-tagged progeny virus containing infectious genomic RNA2014

    • Author(s)
      Katayama K, Murakami K, Sharp TM, Guix S, Oka T, Takai-Todaka R, Nakanishi A, Crawford SE, Atmar RL and Estes MK
    • Journal Title

      PNAS

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Norovirus Binding to Intestinal Epithelial Cells Is Independent of Histo-Blood Group Antigens2013

    • Author(s)
      Kosuke Murakami, Chie Kurihara, Tomoichiro Oka, Takashi Shimoike, Yoshiki Fujii, Reiko Takai Todaka, YoungBin Park, Takaji Wakita, Tsukasa Matsuda, Ryota Hokari, Soichiro Miura, Kazuhiko Katayama
    • Journal Title

      PLoS One

      Volume: 14 Issue: 6 Pages: e66534-e66534

    • DOI

      10.1371/journal.pone.0066534

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proposal for a unified norovirus nomenclature and genotyping.2013

    • Author(s)
      4. Kroneman A, Vega E, Vennema H, Vinjé J, White PA, Hansman G, Green K, Martella V, Katayama K, Koopmans M.
    • Journal Title

      Arch Virol.

      Volume: 158 Issue: 10 Pages: 2059-68

    • DOI

      10.1007/s00705-013-1708-5

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ノロウイルス感染症2013

    • Author(s)
      片山和彦
    • Journal Title

      ノロウイルスの流行のメカニズム感染症

      Volume: vol. 253

    • Related Report
      2013 Final Research Report
  • [Journal Article] ノロウイルス感染のメカニズム2013

    • Author(s)
      片山和彦
    • Journal Title

      食と健康

      Volume: 10月号 Pages: 9-17

    • Related Report
      2013 Final Research Report
  • [Journal Article] hylogenetic analysis of the nonstructural and structural protein encoding region sequences, indicating successive appearance of genomically diverse sapovirus strains from gastroenteritis patients.2013

    • Author(s)
      1. Harada S, Tokuoka E, Kiyota N, Katayama K, Oka T.
    • Journal Title

      Jpn J Infect Dis.

      Volume: 66 Pages: 454-7

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of multiple human sapoviruses from imported frozen individual clams.2013

    • Author(s)
      5. Iizuka S, Takai-Todaka R, Ohshiro H, Kitajima M, Wang Q, Saif LJ, Wakita T, Noda M, Katayama K, Oka T.
    • Journal Title

      Food Environ Virol.

      Volume: 5 Issue: 2 Pages: 119-25

    • DOI

      10.1007/s12560-013-9109-1

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Basis for Broad Detection of Genogroup II Noroviruses by a Monoclonal Antibody That Binds to a Site Occluded in the Viral Particle2012

    • Author(s)
      Grant S. Hansman, David W. Taylor, Ivelin Georgiev, Jeremy R. H. Tame, Sam-Yong Park, Makoto Yamazaki, Fumio Gondaira, Motohiro Miki, Kazuhiko Katayama, Kazuyoshi Murata, and Peter D. Kwong.
    • Journal Title

      Journal of virology

      Volume: 86 Pages: 3635-3646

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Secretory pathway antagonism by calicivirus homologues of Norwalk virus nonstructural protein p22 is restricted to noroviruses.2012

    • Author(s)
      yler M Sharp, Sue E Crawford, Nadim J Ajami, Frederick Neill, Robert L Atmar, Kazuhiko Katayama, Budi Utama, Mary K Estes.
    • Journal Title

      Virology Journal

      Volume: 9;181 Issue: 1 Pages: 1-5

    • DOI

      10.1186/1743-422x-9-181

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural basis for specific recognition of substrates by sapovirus protease.2012

    • Author(s)
      Masaru Yokoyama, Tomoichiro Oka, Hirotatsu Kojima, Tetsuo Nagano, Takayoshi Okabe, Kazuhiko Katayama, Takaji Wakita, Tadahito Kanda and Hironori Sato.
    • Journal Title

      Frontiers in Microbiology

      Volume: 3; 312 Pages: 1-10

    • DOI

      10.3389/fmicb.2012.00312

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] In silico 3D structure analysis accelerates the solution of a real viral structure and antibodies docking mechanism.2012

    • Author(s)
      Motohiro Miki and Kazuhiko Katayama.
    • Journal Title

      Frontiers in Microbiology

      Volume: 3:387 Pages: 1-6

    • DOI

      10.3389/fmicb.2012.00387

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation of four antiseptics using a novel murine norovirus.2012

    • Author(s)
      Matsuhira, T. Kaji, C. Murakami, S. Maebashi, K. Oka, T. Takeda, N. Katayama, K.
    • Journal Title

      Exp Anim.

      Volume: 61 Pages: 35-40

    • NAID

      10030286658

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bioluminescence technologies to detect calicivirus protease activity in cell-free system and in infected cells.2011

    • Author(s)
      Oka, T., Takagi, H., Tohya, Y., Murakami, K., Takeda, N., Wakita, T., Katayama, K.
    • Journal Title

      Antiviral Res.

      Volume: 90 Pages: 9-16

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparative site-directed mutagenesis in the catalytic amino acid triad in calicivirus proteases.2011

    • Author(s)
      Oka, T., Murakami, K., Wakita, T., Katayama, K.
    • Journal Title

      Microbiol Immunol.

      Volume: 55 Pages: 108-14

    • NAID

      10031121448

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystal structures of GII.10 and GII.12 norovirus protruding domains in complex with histo-blood group antigens reveal details for a potential site of vulnerability.2011

    • Author(s)
      Hansman, G. S., Biertumpfel, C., Georgiev, I., McLellan, J. S., Chen, L., Zhou, T., Katayama, K., Kwong, P. D.
    • Journal Title

      Journal of virology

      Volume: 85 Pages: 6687-701

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural basis for norovirus inhibition and fucose mimicry by citrate.2011

    • Author(s)
      Hansman, G. S., Shahzad-Ul-Hussan, S., McLellan, J. S., Chuang, G. Y., Georgiev, I., Shimoike, T., Katayama, K., Bewley, C. A., Kwong, P. D.
    • Journal Title

      J of Virol.

      Volume: 86 Pages: 284-92

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ノロウイルスの小腸上皮細胞への結合メカニズムの解析2013

    • Author(s)
      村上耕介、藤井克樹、戸高玲子、片山和彦
    • Organizer
      第54回日本臨床ウイルス学会
    • Place of Presentation
      岡山県倉敷市
    • Year and Date
      2013-06-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Selective constraints on changes of a norovirus pandemic lineage GII.4_2006b2013

    • Author(s)
      Sato H, Yokoyama M, Motomura K, Nakamura H, Oka T, Katayama K, Takeda N, Noda M, Tanaka T, and the Norovirus Surveillance Group of Japan
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Final Research Report
  • [Presentation] Deep Sequencing-based analysis of minor variants in norovirus infection cases with acute gastroenteritis2013

    • Author(s)
      Motomura K, Ode H, Yokoyama M, Nakamura H, Sato A, Katayama K, Noda M, Takeda N, Tanaka T, Sato H and the Norovirus Surveillance Group of Japan
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural basis of substrate specificity in murine norovirus protease suggested by molecular dynamics simulation2013

    • Author(s)
      Yokoyama M, Oka T, Katayama K, Sato H
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Final Research Report
  • [Presentation] Estes^1 A plasmid based reverse genetics system can drive human and murine norovirus genome replication and produce progeny virus containing reporter tagged infectious genomic RNA2013

    • Author(s)
      Kazuhiko Katayama^<1,2>, Reiko Takai-Todaka, Akira Nakanishi, Kosuke Murakami^<1,2>, Tomoichiro Oka, Susana Guix, Tyler M. Sharp, Robert L. Atmar , Sue E. Crawford, and Mary K
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Final Research Report
  • [Presentation] Study of histo-blood group antigen-independent mechanism of norovirus-cell binding2013

    • Author(s)
      Kosuke Murakami, YoungBin Park, Chie Kurihara, Tomoichiro Oka, Takashi Shimoike, Reiko Takai-Todaka, Takaji Wakita, Tsukasa Matsuda, Ryota Hokari and Katayama Kazuhiko
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of a novel norovirus RT-PCR amplification system corresponding to a consensus norovirus nomenclature 20132013

    • Author(s)
      YoungBin Park, Reiko Takai-Todaka, Kosuke Murakami, Kazuhiko Katayama
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Final Research Report
  • [Presentation] 分子動力学法によるマウスノロウイルスプロテアーゼの基質認識機構の解析2013

    • Author(s)
      横山勝、岡智一郎、片山和彦、佐藤裕徳
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] ノロウイルスの小腸上皮細胞への結合メカニズム2013

    • Author(s)
      村上耕介、戸高玲子、朴英斌、藤井克樹、下池貴志、脇田隆字、栗原千枝、穂苅量太、松田幹、片山和彦
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] カリシウイルスのリバースジェネティックスシステムを用いた感染性粒子の研究2013

    • Author(s)
      戸高玲子、村上耕介、岡智一郎、高木弘隆、朴英斌、下池貴志、藤井克樹、脇田隆字、中西章、片山和彦
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of a novel norovirus RT-PCR amplification system corresponding to a consensus norovirus nomenclature 20132013

    • Author(s)
      Youngbin Park, Kazuhiko Katayama
    • Organizer
      61th annual meeting of the Japanese society for virology
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] A plasmid based reverse genetics system can drive human and murine norovirus genome replication and produce progeny virus containing reporter tagged infectious genomic RNA.2013

    • Author(s)
      Kazuhiko Katayama1,2, Reiko Takai-Todaka2, Akira Nakanishi3, Kosuke Murakami1,2, Tomoichiro Oka2, Susana Guix1, Tyler M. Sharp1, Robert L. Atmar 1, Sue E. Crawford1, and Mary K. Estes1
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Study of histo-blood group antigen-independent mechanism of norovirus-cell binding.2013

    • Author(s)
      Kosuke Murakami1, YoungBin Park1, Chie Kurihara2, Tomoichiro Oka1, Takashi Shimoike1, Reiko Takai-Todaka1, Takaji Wakita1, Tsukasa Matsuda3, Ryota Hokari2 and Katayama Kazuhiko1
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of a novel norovirus RT-PCR amplification system corresponding to a consensus norovirus nomenclature 2013.2013

    • Author(s)
      YoungBin Park, Reiko Takai-Todaka, Kosuke Murakami, Kazuhiko Katayama.
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Deep Sequencing-based analysis of minor variants in norovirus infection cases with acute gastroenteritis.2013

    • Author(s)
      Motomura K, Ode H, Yokoyama M, Nakamura H, Sato A, Katayama K, Noda M, Takeda N, Tanaka T, Sato H and the Norovirus Surveillance Group of Japan.
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Selective constraints on changes of a norovirus pandemic lineage GII.4_2006b.2013

    • Author(s)
      Sato H, Yokoyama M, Motomura K, Nakamura H, Oka T, Katayama K, Takeda N, Noda M, Tanaka T, and the Norovirus Surveillance Group of Japan. Selective constraints on changes of a norovirus pandemic lineage GII.4_2006b.
    • Organizer
      The Fifth International Calicivirus Conference
    • Place of Presentation
      Beijing, China.
    • Related Report
      2013 Annual Research Report
  • [Presentation] カリシウイルスのユニバーサルなプラスミドベースリバースジェネティックスシステム2012

    • Author(s)
      戸高玲子、村上耕介、岡智一郎、高木 弘隆、朴英斌、下池貴志、藤井克樹、脇田隆字、中西章、片山和彦
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] パンデミックノロウイルスの変化の制約2012

    • Author(s)
      佐藤裕徳、本村和嗣、横山勝、椎野禎一郎、中村浩美、岡智一郎、片山和彦、野田衛、田中智之、Norovirus Surveillance Group of Japan
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] ノロウイルスのCaco-2細胞への結合に関与するタンパク質の探索2012

    • Author(s)
      村上耕介、岡智一郎、下池貴志、藤井克樹、Park YoungBin、戸高玲子、脇田隆字、松田幹、片山和彦
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] マウスノロウイルス感染細胞内のウイルス蛋白質とそのゲノムRNAの局在2012

    • Author(s)
      下池貴志、戸高玲子、村上耕介、岡智一郎、高木 弘隆、朴英斌、藤井克樹、脇田隆字、中西章、片山和彦
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] Visualization of Murine Norovirus proteins and its genome RNA2012

    • Author(s)
      Shimoike T, Todaka R, Murakami K, Takagi H, Park Y, Fujii Y, Wakita T and Katayama K.
    • Organizer
      American Society for Cell Biology
    • Place of Presentation
      San Francisco
    • Related Report
      2012 Research-status Report
  • [Presentation] Development of plasmid based reverse genetics system that can drive MNV and FCV genome by human promoter EF-1α2012

    • Author(s)
      Todaka R, Murakami K,Oka T, Takagi H, Park Y, Fujii Y, Wakita T, Nakanishi A, Katayama K.
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Screening for candidate receptor on Caco-2 involved in norovirus binding by mass spectrometry from different approaches2012

    • Author(s)
      Murakami K, Oka T, Shimoike T, Fujii Y, Park Y, Todaka R, Wakita T, Matsuda T, and Katayama K.
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Visualization of Murine Norovirus replication complex in RAW264.7 cells2011

    • Author(s)
      Takashi Shimoike, Hirotaka Takagi, Tomoichiro Oka, Kosuke Murakami, Takaji Wakita, and Kazuhiko Katayama
    • Organizer
      IUMS
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] Norovirus as gastrointestinal flu2011

    • Author(s)
      Kazuhiko Katayama
    • Organizer
      IUMS(招待講演)
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] Development of a novel norovirus RT-PCR amplification system corresponding to a consensus norovirus nomenclature 2013

    • Author(s)
      YoungBin Park, Reiko Takai-Todaka, Kosuke Murakami, Kazuhiko Katayama
    • Organizer
      Japan-Taiwan joint meeting
    • Place of Presentation
      Shinjuku, Tokyo, JAPAN
    • Related Report
      2013 Final Research Report
  • [Presentation] 「新しい作用機構の抗ウイルス薬開発への取り組み-ウイルス感染症に挑む-」ノロウイルスたんぱく質構造と抗ウイルス薬

    • Author(s)
      片山和彦
    • Organizer
      第42回ヒューマンサイエンス総合研究セミナー
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi