Study of human norovirus replication and pathogenicity
Project/Area Number |
23590556
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 病原性 / 細胞内同多 / 機能 / 細胞内動態 |
Research Abstract |
We established a new reverse genetics system driven by a mammalian promoter, that functions without helper virus. The complete genome of the HuNoV GII.3 U201, Saga1, TCH, NV68 and also MNV-S7 strain was cloned downstream of an EF-1 alpha mammalian promoter. These constructs produced progeny viruses produced from cells that contained the complete NoV genomic RNA, VP1, VP2 and VPg were detected in isopycnic gradients of CsCl at the same density as native infectious NoV particles from a patient's stool. A GFP reporter construct containing the GFP gene in ORF1 produced complete virions that contain VPg linked RNA. RNA from virions containing the encapsidated GFP genomic RNA was successfully transfected back into cells producing fluorescent puncta indicating that the encapsidated RNA is replication competent. Our results are useful to develop of an antiviral medicine which targeted these in future.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Norovirus Binding to Intestinal Epithelial Cells Is Independent of Histo-Blood Group Antigens2013
Author(s)
Kosuke Murakami, Chie Kurihara, Tomoichiro Oka, Takashi Shimoike, Yoshiki Fujii, Reiko Takai Todaka, YoungBin Park, Takaji Wakita, Tsukasa Matsuda, Ryota Hokari, Soichiro Miura, Kazuhiko Katayama
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Journal Title
PLoS One
Volume: 14
Issue: 6
Pages: e66534-e66534
DOI
Related Report
Peer Reviewed
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[Journal Article] Structural Basis for Broad Detection of Genogroup II Noroviruses by a Monoclonal Antibody That Binds to a Site Occluded in the Viral Particle2012
Author(s)
Grant S. Hansman, David W. Taylor, Ivelin Georgiev, Jeremy R. H. Tame, Sam-Yong Park, Makoto Yamazaki, Fumio Gondaira, Motohiro Miki, Kazuhiko Katayama, Kazuyoshi Murata, and Peter D. Kwong.
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Journal Title
Journal of virology
Volume: 86
Pages: 3635-3646
Related Report
Peer Reviewed
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[Journal Article] Bioluminescence technologies to detect calicivirus protease activity in cell-free system and in infected cells.2011
Author(s)
Oka, T., Takagi, H., Tohya, Y., Murakami, K., Takeda, N., Wakita, T., Katayama, K.
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Journal Title
Antiviral Res.
Volume: 90
Pages: 9-16
Related Report
Peer Reviewed
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[Journal Article] Crystal structures of GII.10 and GII.12 norovirus protruding domains in complex with histo-blood group antigens reveal details for a potential site of vulnerability.2011
Author(s)
Hansman, G. S., Biertumpfel, C., Georgiev, I., McLellan, J. S., Chen, L., Zhou, T., Katayama, K., Kwong, P. D.
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Journal Title
Journal of virology
Volume: 85
Pages: 6687-701
Related Report
Peer Reviewed
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[Journal Article] Structural basis for norovirus inhibition and fucose mimicry by citrate.2011
Author(s)
Hansman, G. S., Shahzad-Ul-Hussan, S., McLellan, J. S., Chuang, G. Y., Georgiev, I., Shimoike, T., Katayama, K., Bewley, C. A., Kwong, P. D.
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Journal Title
J of Virol.
Volume: 86
Pages: 284-92
Related Report
Peer Reviewed
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[Presentation] Selective constraints on changes of a norovirus pandemic lineage GII.4_2006b2013
Author(s)
Sato H, Yokoyama M, Motomura K, Nakamura H, Oka T, Katayama K, Takeda N, Noda M, Tanaka T, and the Norovirus Surveillance Group of Japan
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China
Related Report
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[Presentation] Deep Sequencing-based analysis of minor variants in norovirus infection cases with acute gastroenteritis2013
Author(s)
Motomura K, Ode H, Yokoyama M, Nakamura H, Sato A, Katayama K, Noda M, Takeda N, Tanaka T, Sato H and the Norovirus Surveillance Group of Japan
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China
Related Report
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[Presentation] Estes^1 A plasmid based reverse genetics system can drive human and murine norovirus genome replication and produce progeny virus containing reporter tagged infectious genomic RNA2013
Author(s)
Kazuhiko Katayama^<1,2>, Reiko Takai-Todaka, Akira Nakanishi, Kosuke Murakami^<1,2>, Tomoichiro Oka, Susana Guix, Tyler M. Sharp, Robert L. Atmar , Sue E. Crawford, and Mary K
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China
Related Report
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[Presentation] A plasmid based reverse genetics system can drive human and murine norovirus genome replication and produce progeny virus containing reporter tagged infectious genomic RNA.2013
Author(s)
Kazuhiko Katayama1,2, Reiko Takai-Todaka2, Akira Nakanishi3, Kosuke Murakami1,2, Tomoichiro Oka2, Susana Guix1, Tyler M. Sharp1, Robert L. Atmar 1, Sue E. Crawford1, and Mary K. Estes1
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China.
Related Report
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[Presentation] Deep Sequencing-based analysis of minor variants in norovirus infection cases with acute gastroenteritis.2013
Author(s)
Motomura K, Ode H, Yokoyama M, Nakamura H, Sato A, Katayama K, Noda M, Takeda N, Tanaka T, Sato H and the Norovirus Surveillance Group of Japan.
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China.
Related Report
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[Presentation] Selective constraints on changes of a norovirus pandemic lineage GII.4_2006b.2013
Author(s)
Sato H, Yokoyama M, Motomura K, Nakamura H, Oka T, Katayama K, Takeda N, Noda M, Tanaka T, and the Norovirus Surveillance Group of Japan. Selective constraints on changes of a norovirus pandemic lineage GII.4_2006b.
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China.
Related Report
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