Analysis of the early steps of enterovirus 71 infection

• Project/Area Number: 23590557
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: The University of Tokyo (2012-2013); Tokyo Metropolitan Organization for Medical Research (2011)
• Principal Investigator: YAMAYOSHI Seiya 東京大学, 医科学研究所, 特任助教 (50529534)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: エンテロウイルス71 / レセプター / SCARB2 / PSGL-1 / 機能比較 / EV71 / 感染受容体 / PSGL1

Research Abstract

Human scavenger receptor class B member 2 (SCARB2) and P-selectin glycoprotein ligand-1 (PSGL-1) have been reported te be receptors for enterovirus 71. I functionally compared these receptors. Enterovirus 71 infects more efficiently via SCARB2 than PSGL-1. The binding affinity of PSGL-1 to viral particles is stronger than that of SCARB2. The conformational alteration of enterovirus 71 is triggered by incubation with SCARB2 under acidic conditions but not by incubation with PSGL-1 under any condition which we tested.
These data suggest that SCARB2 is an authentic receptor for enterovirus 71 and PSGL-1 may act as an attachment molecule for enterovirus 71 on the cell surface.

Research Products

• [Journal Article] Functional Comparison of SCARB2 and PSGL1 as Receptors for EV71 (2013)
  • Author(s): Yamayoshi, S., S. Ohka, K. Fujii and S. Koike
  • Journal Title: Journal of Virology Volume: 87 Issue: 6 Pages: 3335-3347
  • DOI: 10.1128/jvi.02070-12
  • Peer Reviewed
• [Journal Article] Transgenic mous model for the study of enterovirus 71 pathogenesis (2013)
  • Author(s): Fujii K, Nagata N, SatoY, Ong KC, Wong KT, Yamayoshi S, Shimanuki M, Shitara H, Taya C, Koike S
  • Journal Title: Proceedings of the National Academy of Science United States of America Volume: 110 Issue: 36 Pages: 14753-14758
  • DOI: 10.1073/pnas.1217563110
  • Peer Reviewed
• [Journal Article] Human SCARB2-Dependent Infection by Coxsackievirus A7, A14, and A16 and Enterovirus 71 (2012)
  • Author(s): Seiya Yamayoshi
  • Journal Title: Journal of Virology Volume: VOL 86 Issue: 10 Pages: 5686-5696
  • DOI: 10.1128/jvi.00020-12
  • Peer Reviewed
• [Journal Article] Scavenger receptor B2 as a receptor for hand, foot, and mouth disease and severe neurological diseases (2012)
  • Author(s): Yamayoshi S, Fujii K, Koike S
  • Journal Title: Frontiers in Virology Volume: 3 Pages: 32-32
  • DOI: 10.3389/fmicb.2012.00032
  • Peer Reviewed
• [Journal Article] Identification of the Human SCARB2 Region That Is Important for Enterovirus 71 Binding and Infection (2011)
  • Author(s): Yamayoshi S, Koike S
  • Journal Title: Journal of Virology Volume: 85 Issue: 10 Pages: 4937-4936
  • DOI: 10.1128/jvi.02358-10
  • Peer Reviewed
• [Presentation] 2つのエンテロウイルス71感染受容体SCARB2とPSGL1の機能比較 (2012)
  • Author(s): 山吉誠也、大岡静衣、藤井健、小池智
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪府
  • Year and Date: 2012-11-14
• [Presentation] EV71感受性マウスモデルの作出と解析 (2012)
  • Author(s): 藤井健、永田典代、山吉誠也、島貫碧、設楽浩志、多屋長治、小池智
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪府
  • Year and Date: 2012-11-14
• [Presentation] エンテロウイルス71感染受容体の機能比較 (2012)
  • Author(s): 山吉誠也、大岡静衣、藤井健、小池智
  • Organizer: 第9回ウイルス学キャンプin湯河原
  • Place of Presentation: 静岡県
  • Year and Date: 2012-07-11
• [Presentation] 手足口病の原因ウイルスの感染受容体 (2012)
  • Author(s): 山吉誠也
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 埼玉県
  • Year and Date: 2012-03-28
• [Presentation] エンテロウイルス71感染受容体の機能比較 (2012)
  • Author(s): 山吉誠也、藤井健、小池智
  • Organizer: 感染症若手フォーラム
  • Place of Presentation: 長崎県
  • Year and Date: 2012-02-04
• [Presentation] 手足口病の原因ウイルスの感染受容体 (2012)
  • Author(s): 山吉誠也
  • Organizer: 第153回日本獣医学会学術集会(招待講演)
  • Place of Presentation: 埼玉県大宮市
• [Presentation] エンテロウイルス７１感染受容体の機能比較 (2012)
  • Author(s): 山吉誠也、藤井健、小池智
  • Organizer: 感染症若手フォーラム
  • Place of Presentation: 長崎県長崎市
• [Presentation] CVA7、CVA14、CVA16およびEV71はSCARB2依存的に細胞に感染する (2011)
  • Author(s): 山吉誠也、藤井健、小池智
  • Organizer: 第8回ウイルス学キャンプin湯河原
  • Place of Presentation: 静岡県
  • Year and Date: 2011-11-07
• [Presentation] Human SCARB2-dependent infection of clinical isolates of Coxsackievirus A14, A16 and Enterovirus 71 (2011)
  • Author(s): S. Yamayoshi, S Iizuka, T. Yamashita, H. Minagawa, K. Sanjoh, N. Katsushima, T. Itagaki, K. Mizuta, Y. Nagai, M. Okamoto, H. Nishimura, K. Fujii, S. Koike
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: 北海道
  • Year and Date: 2011-09-15
• [Presentation] エンテロウイルス71感染受容体の機能解析 (2011)
  • Author(s): 山吉誠也、藤井健、小池智
  • Organizer: 第15回神経ウイルス研究会
  • Place of Presentation: 石川県
  • Year and Date: 2011-05-19
• [Presentation] Human SCARB2-dependent infection of clinical isolates of Coxsackievirus A14, A16 and Enterovirus 71 (2011)
  • Author(s): Yamayoshi, S., S. Iizuka, T. Yamashita, H. Minagawa, K. Sanjoh, N. Katsushima, T. Itagaki, K. Mizuta, Y. Nagai, M. Okamoto, H. Nishimura, K. Fujii, S. Koike
  • Organizer: International Congress of Virology
  • Place of Presentation: 北海道札幌市
• [Presentation] エンテロウイルス７１感染受容体の機能解析 (2011)
  • Author(s): 山吉誠也、藤井健、小池智
  • Organizer: 第15回神経ウイルス研究会
  • Place of Presentation: 石川県金沢市
• [Presentation] Functional Analyses of Scavenger Receptor B2 as a receptor for Enterovirus 71
  • Author(s): S. Yamayoshi, K. Fujii and S. Koike
  • Organizer: Europic 2012
  • Place of Presentation: フランス、サンラファエル
• [Presentation] Establishment of a transgenic mouse model for EV71 infection
  • Author(s): K. Fujii, N. Nagata, S. Yamayoshi, M. Shimanuki, H. Shitara, C. Taya and S. Koike
  • Organizer: Europic 2012
  • Place of Presentation: フランス、サンラファエル
• [Presentation] エンテロウイルス７１感染受容体の機能比較
  • Author(s): 山吉誠也、大岡静衣、藤井健、小池智
  • Organizer: 第9回ウイルス学キャンプ in 湯河原
  • Place of Presentation: 静岡県
• [Presentation] ２つのエンテロウイルス７１感染受容体SCARB2とPSGL1の機能比較
  • Author(s): 山吉誠也、大岡静衣、藤井健、小池智
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪府
• [Presentation] ＥＶ７１感受性マウスモデルの作出と解析
  • Author(s): 藤井健、永田典代、山吉誠也、島貫碧、設楽浩志、多屋長治、小池智
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪府