Elucidation of interorganelle network in RLRs-mediated innate immunity

• Project/Area Number: 23590560
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Immunology
• Research Institution: Hirosaki University
• Principal Investigator: MATSUMIYA Tomoh 弘前大学, 医学(系)研究科(研究院), 助教 (30344592)
• Co-Investigator(Kenkyū-buntansha): IMAIZUMI Tadaatsu 弘前大学, 大学院医学研究科, 教授 (90232602) ; SATOH Kei 弘前大学, 事務局, 学長 (20125438)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 自然免疫 / ウイルス学 / 細胞生物学 / ミトコンドリア / 抗ウイルスシグナル / RLR / ウイルスセンサー / 細胞小器官 / 細胞内小器官 / RLRs

Research Abstract

Crucial role of mitochondria has been proven in RLRs (RIG-I-like receptors)-mediated antiviral signaling pathway. Organelles including mitochondria are known to form interorganelle network. We investigated the formation of the interorganelle network in the RLRs-mediated antiviral signaling. We found that some organelles were close upon activation of the antiviral signaling; however no contact between goldi or endosomes and mitochondria was observed in response to dsRNA introduction. These results suggested that transduction of signaling molecules may be essential in the interorganelle network.

Research Products

• [Journal Article] Periosteum-derived cells respond to mechanical stretch and activate Wnt and BMP signaling pathways (2014)
  • Author(s): Ito R, Matsumiya T, Kon T, Narita N, Kubota K, Sakaki H, Ozaki T, Imaizumi T, Kobayashi W, Kimura H
  • Journal Title: Biomed Res Volume: 35(1) Pages: 69-79
• [Journal Article] Role of type I- and type II-interferon in expression of melanoma differentiation-associated gene-5 in HSC-3 oral squamous carcinoma cells (2014)
  • Author(s): Kon T , Matsumiya T, Hayakari R, Narita N, Ito R, Kubota K, Sakaki H, Yoshida H, Imaizumi T, Kobayashi W, Kimura H
  • Journal Title: Biomed Res Volume: 35(1) Pages: 9-16
  • NAID: 130004903864
• [Journal Article] Carnosic acid suppresses the production of amyloid-beta 1-42 and 1-43 by inducing an alpha-secretase TACE/ADAM17 in U373MG human astrocytoma cells. (2014)
  • Author(s): Yoshida H, Meng P, Matsumiya T, Tanji K, Hayakari R, Xing F, Wang L, Tsuruga K, Tanaka H, Mimura J, Kosaka K, Itoh K, Takahashi I, Imaizumi T
  • Journal Title: Neurosci Res Volume: 79 Pages: 89-94
  • DOI: 10.1016/j.neures.2013.11.004
  • Peer Reviewed
• [Journal Article] Periosteum-derived cells respond to mechanical stretch and activate Wnt and BMP signaling pathways. (2014)
  • Author(s): Ito R, Matsumiya T, Kon T, Narita N, Kubota K, Sakaki H, Ozaki T, Imaizumi T, Kobayashi W, Kimura H.
  • Journal Title: Biomed Res Volume: 35 Pages: 69-79
  • Peer Reviewed
• [Journal Article] Role of type I- and type II-interferon in expression of melanoma differentiation-associated gene-5 in HSC-3 oral squamous carcinoma cells. (2014)
  • Author(s): Matsumiya T, Hayakari R, Narita N, Ito R, Kon T, Kubota K, Sakaki H, Yoshida H, Imaizumi T, Kobayashi W, Kimura H.
  • Journal Title: Biomed Res Volume: 35 Pages: 9-16
  • NAID: 130004903864
  • Peer Reviewed
• [Journal Article] Novel role for molecular transporter importin 9 in posttranscriptional regulation of IFN-ε expression. (2013)
  • Author(s): Matsumiya T, Xing F, Ebina M, Hayakari R, Imaizumi T, Yoshida H, Kikuchi H, Topham MK, Satoh K, Stafforini DM.
  • Journal Title: J Immunol Volume: 191 Issue: 4 Pages: 1907-15
  • DOI: 10.4049/jimmunol.1201925
  • Peer Reviewed
• [Journal Article] How are STAT1 and cholesterol metabolism associated in antiviral responses? (2013)
  • Author(s): Matsumiya T, Imaizumi T
  • Journal Title: J AK-STAT Volume: 2(3) Issue: 3 Pages: e24189-e24189
  • DOI: 10.4161/jkst.24189
• [Journal Article] Foreign RNA induces the degradation of mitochondrial antiviral signaling protein (MAVS)the role of intracellular antiviral factors. (2012)
  • Author(s): Xing F, Matsumiya T, Onomoto K, Hayakari R, Imaizumi T, Yoshida H, Yoneyama M, Fijita T, Satoh K
  • Journal Title: PLoS One Volume: 7(9) Issue: 9 Pages: e45136-e45136
  • DOI: 10.1371/journal.pone.0045136
  • Peer Reviewed
• [Journal Article] Double-stranded RNA induces biphasic STAT1 phosphorylation by both type I IFN-dependent and type I IFN-independent pathways (2012)
  • Author(s): Dempoya J, Matsumiya T, Imaizumi T, Hayakari R, Xing F, Yoshida H, Okumura K, Satoh K
  • Journal Title: J Virol Volume: 86(23) Issue: 23 Pages: 12760-12769
  • DOI: 10.1128/jvi.01881-12
  • Peer Reviewed
• [Journal Article] Basic-helix-loop-helix transcription factor DEC2 constitutes negative feedback loop in IFN-β-mediated inflammatory responses in human mesangial cells (2011)
  • Author(s): Imaizumi T, Sato F, Tanaka H, Matsumiya T, Yoshida H, Yashiro-Aizawa T, Tsuruga K, Hayakari R, Kijima H, Satoh K
  • Journal Title: Immunol Lett Volume: 136(1) Pages: 37-43
• [Journal Article] Polyinosinic-polycytidylic acid induces the expression of interferon-stimulated gene 20 in mesangial cells (2011)
  • Author(s): Imaizumi T, Tanaka H, Mechti N, Matsumiya T, Yoshida H, Sato F, Aizawa-Yashiro T, Tsuruga K, Hayakari R, Satoh K
  • Journal Title: Nephron Exp Nephrol Volume: 119(2) Issue: 2 Pages: e40-e48
  • DOI: 10.1159/000328923
  • Peer Reviewed
• [Journal Article] Edaravone and carnosic acid synergistically enhance the expression of nerve growth factor in human astrocytes under hypoxia/reoxygenation (2011)
  • Author(s): Yoshida H, Mimura J, Imaizumi T, Matsumiya T, Ishikawa A, Metoki N, Tanji K, Ota K, Hayakari R, Kosaka K, Itoh K, Satoh K
  • Journal Title: Neurosci Res Volume: 69 Issue: 4 Pages: 291-298
  • DOI: 10.1016/j.neures.2010.12.016
  • Peer Reviewed
• [Journal Article] Basic-helix-loop-helix transcription factor DEC2 constitutes negative feedback loop in IFN-β-mediated inflammatory responses in human mesangial cells. (2011)
  • Author(s): Imaizumi T, Sato F, Tanaka H, Matsumiya T, Yoshida H, Yashiro-Aizawa T, Tsuruga K, Hayakari R, Kijima H, Satoh K.
  • Journal Title: Immunol Lett. Volume: 136 (1) Pages: 37-43
  • Peer Reviewed
• [Journal Article] vone and carnosic acid synergistically enhance the expression of nerve growth factor in human astrocytes under hypoxia/reoxygenation. (2011)
  • Author(s): Yoshida H, Mimura J, Imaizumi T, Matsumiya T, Ishikawa A, Metoki N, Tanji K, Ota K, Hayakari R, Kosaka K, Itoh K, Satoh K.
  • Journal Title: Neurosci Res Volume: 69 (4) Pages: 291-298
  • Peer Reviewed
• [Journal Article] Polyinosinic-polycytidylic acid induces the expression of interferon-stimulated gene 20 in mesangial cells. (2011)
  • Author(s): Imaizumi T, Tanaka H, Mechti N, Matsumiya T, Yoshida H, Sato F, Aizawa-Yashiro T, Tsuruga K, Hayakari R, Satoh K.
  • Journal Title: Nephron Exp Nephrol. Volume: 119 (2)
  • Peer Reviewed
• [Presentation] Multi-step regulation of STAT1 phosphorylation in response to double-stranded RNA (2013)
  • Author(s): Matsumiya T, Dempoya J, Xing F, Hayakari R, Yoshida H, Imaizumi T
  • Organizer: 15th International Congress of Immunology
  • Place of Presentation: Milan, Italy
• [Presentation] Biphasic STAT1 phosphorylation in response to double-stranded RNA (2013)
  • Author(s): Matsumiya T, Dempoya J, Xing F, Hayakari R, Imaizumi T, Yoshida H, Satoh K
  • Organizer: Immunology 2013, American Association of Immunologists Annual Meeting
  • Place of Presentation: Hawaii, USA
• [Presentation] Biphasic STAT1 phosphorylation in response to double-stranded RNA (2013)
  • Author(s): Matsumiya T, Dempoya J, Xing F, Hayakari R, Imaizumi T, Yoshida H, Satoh K
  • Organizer: Immunology 2013
  • Place of Presentation: Honolulu, Hawaii, USA
• [Presentation] Multi-step regulation of STAT1 phosphorylation in response to double-stranded RNA (2013)
  • Author(s): Matsumiya T, Dempoya J, Xing F, Hayakari R, Yoshida H, Imaizumi T
  • Organizer: 15th International congress of immnology
  • Place of Presentation: Milan, Italy
• [Presentation] Involvement of neuronal differentiation in intracellular antiviral responses (2012)
  • Author(s): Matsumiya T, Hayakari R, Imaizumi T, Yoshida H
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋市
• [Presentation] Involvement of neuronal differentiation in intracellular antiviral responses. (2012)
  • Author(s): Tomoh Matsumiya, Ryo Hayakari, Tadaatsu Imaizumi and Hidemi Yoshida
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（愛知県）