Higher-order Chromatin Structure Controls T Helper Cell Differentiation
| Project/Area Number |
23590562
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SEKIMATA Akiko 山形大学, 医学部, 准教授 (50321823)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 遺伝子発現 / エピジェネティクス / 細胞分化 / クロマチン / ヘルパーＴ細胞 / サイトカイン / 転写制御 / 分化制御 / 国際情報交換 / 高次クロマチン構造 / 国際情報交流 |
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| Research Abstract |
The formation of higher-order chromatin structure composed of cis-regulatory elements including enhancers, silencers, and insulators play important roles in regulating cell lineage-specific activation and repression of genes. Cytokine genes expressed in T-helper cells are particularly attractive models to study lineage specific regulation by higher-order chromatin structure. In this study, we identified several cis-regulatory elements at the mouse IFN-g gene locus including IFN-g and IL-22. These elements functionally regulated lineage-specific gene expression as enhancers and silencers. Moreover, we try to generate mice with a conditional deletion of an insulator located 70 kb upstream of the IFN-g gene that is a site for recruitment of the factor CTCF, which may enable us to define the epigenetic and functional consequences of lineage-specific gene expression.
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Report
(4 results)
Research Products
(9 results)
