| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Research Abstract |
Zinc (Zn) is an essential nutrient and its deficiency causes immunodeficiency. Electron microscopic studies demonstrated that mast cell granules are rich in Zn. However, the precise roles and molecular mechanism(s) of Zn`s function in mast cells have not been clarified. It has been considered that Zn is supplied to granule by Zn transporter. We identified that Zn transporter ZnT2/Slc30a2 was localized at granule membrane in mast cell. To understand role of granule`s Zn in mast cell, we generated ZnT2-KO mice. We found that survival rate in cecal ligation and puncture was diminished in ZnT2-KO mice.
It has reported Zn is required for activity of CPA3 (carboxypeptidase3) in mast cell and loss of CPA3 activity causes endothelin-induced sepsis shock. Consistent with this report, CPA3 activity was decreased in ZnT2-KO mast cell. In this study, we showed that ZnT2 is important for survival from sepsis and required for Zn-dependent CPA3 activity.
|
