| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We designed a new class of branched amphiphilic peptide capsules(BAPC) that self-assemble into extremely stable nanospheres.BAPC mixed with gene were delivered to cells without cytotoxicity. But transfectionｓ were not enough high to apply gene therapy for mice.We investigated the biophysical properties and transfection efficiency for BAPC form peptiplexes with DNA. We found that, in the presence of double stranded plasmid DNA,BAPC are unable to form.Instead depending of the peptide/DNA ratios,the peptides either coat the plasmid surface forming nanofibers (high peptide to DNA ratio) or condense the plasmid into nanometer-sized compacted structures (low peptide to DNA ratios). Different gene delivery efficiencies are observed for the two types of assemblies.The compacted structures display much higher transfection in HeLa cells.This level of transfection is greater than that observed for a lipid-based reagent when the total number of viable transfected cells is taken into account.
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