| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
To investigate the roles of stemness-related signals in the growth of leukemia cells, the signals were activated by the ligand-stimulation and were suppressed by adding the inhibitors or siRNA (small interfering RNA) in culture systems of leukemia cells. Activation of Hedgehog signal did not affect the short-term growth while it promoted the self-renewal of clonogenic cells. Activation of BMP (Bone Morphogenetic Protein) signal promoted the growth of some samples while it suppressed the colony formation. Suppression of Notch, Hedgehog, Wnt, mTOR (mammalian Target of Rapamycin), and HIF (Hypoxia Inducible Factor) signals by adding each inhibitor or siRNA suppressed the growth. We found the cross-talk between these signals. The analysis of Notch expression by flow cytometry could not quantitate the leukemia stem cells.
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