Analysis of phospholipid metabolism in interstitial pneumonia and development of a laboratory assay using mass spectrometry
| Project/Area Number |
23590665
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Takayuki 信州大学, 医学部, 教授 (80238815)
HONGO Minoru 信州大学, 医学部, 教授 (40209317)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 臨床化学 / 脂質 / 質量分析 / リン脂質 / MALDI-TOF MS / 肺胞気管支洗浄液 / 脂質分解酵素 / 肺胞洗浄液 |
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| Research Abstract |
Bronchoalveolar lavage fluid (BALF) is used for diagnosis and determining clinical condition in interstitial pneumonia, but the relations between BAL lipid composition and clinical condition are unclear. We developed an assay for determining lipid species, fatty acid composition, and lipid metabolic enzyme activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and gas chromatography mass spectrometry. The methods were rapid, simple, and accurate with very small samples. The BALF phospholipid profile was determined by mass spectrometry. We could detect changes in metabolism of phospholipid species in BALF by comparing the phospholipid profile of the serum with BAL. The results may be useful as an index to determined phospholipid metabolic disorders in pulmonary surfactant and alveolar cells.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] High concentrations of omega-3 fatty acids are associated with the development of atrial fibril- lation in the Japanese population2013
Author(s)
Tomita T, Hata T, Takeuchi T, Oguchi Y, Okada A, Aizawa K, Koshikawa M, Ota giri K, Motoki H, Kasai H, Izawa A, Koyama J, Hongo M, Ikeda U
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Journal Title
Heart Vessels
Volume: 28(4)
Pages: 497-504
Related Report
Peer Reviewed
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[Presentation] 気管支肺胞洗浄液の質量分析2011
Author(s)
日高宏哉, 山下峰, 大槻晋也, 川崎健治, 菅野光俊, 本田孝行
Organizer
第58回日本臨床検査医学会
Place of Presentation
岡山
Year and Date
2011-11-18
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