| Project/Area Number |
23590666
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
IINO KAZUMI 浜松医科大学, 医学部附属病院, 助教 (90402263)
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| Co-Investigator(Kenkyū-buntansha) |
MAEKAWA Masato 浜松医科大学, 医学部, 教授 (20190291)
OKI Yutaka 浜松医科大学, 医学部, 教授 (20169204)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 褐色細胞腫 / パラガングリオーマ / メチル化解析 / 悪性褐色細胞腫 / メチル化特異的PCR / アンプリコンシークエンス / プロテオーム解析 |
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| Research Abstract |
We investigated methylation of malignant pheochromocytoma (PCC) and paraganglioma (PGL) tumors. We performed Infinium HumanMethylation450 Bead Chip array, which covers 485,577 CpG sites using DNA samples extracted from resected PCC/PGL tissues.
From the results of the Bead Chip array, we selected 12 CpG sites as hypermethylated and 16 CpG sites as hypomethylated candidates. Successively performed semiquantitative methylation-PCR revealed that, of the 28, three CpG sites were selected as possible epigenomic contributors to canceration of PCC/PGL. (one hypermethylated (cg02119938) and two hypomethylated sites (cg16918905, cg26870725). ) The cg02119938 is related to ACSBG1 (acyl-CoA synthetase bubblegum family member 1) gene, and cg26870725 is related to the MAST1 (microtubule associated serine-threonine kinase 1) gene. We showed here three possible CpG sites, in which aberrant promoter methylation/demethylation might be involved in silencing/expressing of cancer-related genes.
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