Basic evaluation of diagnosis of nucleic acid metabolizing enzyme deficiency by flow cytometry
| Project/Area Number |
23590668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Mie University |
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Principal Investigator |
NOBORI Tsutomu 三重大学, 医学(系)研究科(研究院), 特任教授 (60106995)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | MTAP / 酵素欠損 / プリン代謝 / フローサイトメトリー / 選択的化学療法 / コンパニオン診断薬 / プロモーターメチル化 / DNAメチル化 / 個別化医療 / エピトープマッピング / 形質転換細胞 |
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| Research Abstract |
Methylthioadeosine phosphorylase (MTAP) is frequently deficient in a variety of malignancies, although the enzyme is active in normal cells or tissues. This difference in enzyme activities can be exploited to design the tumor-specific chemotherapy.
We tried to develop the diagnostic method of MTAP enzyme deficiency which can be easily applied to clinical samples compared to genetic diagnosis. Anti-MTAP monoclonal antibody which we generated worked well with certain cell lines, whereas it did not with other cell lines. This might be caused by the difference in the amount of MTAP protein expressed.
Since MTAP deficiency is known to be caused by the gene deletion or promoter hypermethylation, we developed the rapid high-pressure liquid chromatography (HPLC) to detect methylated DNA. PCR products were amplified from the methyl sulfite-modified DNA and PCR products with single nucleotide substituions were separated by HPLC.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Clinical Utility of a panfungal polymerase chain reaction assay for invasive fungal diseases in patients with hematologic disorders2013
Author(s)
Sugawawa Y, Nakase K, Nakamura A, Ohishi K, Sugimoto Y, Fujieda A, Monma F, Suzuki K, Masuya M, Matsushima Y, Wada H, Nobori T, Katayama N
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Journal Title
European Journal of Hematology
Volume: vol. 90
Pages: 331-339
Related Report
Peer Reviewed
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[Journal Article] Clinical utility of a panfungal polymerase chain reaction assay for invasive fungal diseases in patients with hematologic disorders2013
Author(s)
Sugawara Y, Nakase K, Nakamura A, Ohishi K, Sugimoto Y, Fujieda A, Monma F, Suzuki K, Masuya M, Matsushima Y, Wada H, Nobori T, Katayama N
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Journal Title
European Journal of Hematology
Volume: 90
Pages: 331-339
Related Report
Peer Reviewed
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