| Project/Area Number |
23590682
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
MAEKITA TAKAO 和歌山県立医科大学, 医学部, 講師 (10326358)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ICHINOSE Masao 和歌山県立医科大学, 医学部, 教授 (50143425)
|
|---|
| Project Period (FY) |
2011-04-28 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | エピジェネティクス / 胃癌 / リスクマーカー / エピジェネテイック / メチル化 / 胃がん / ヘリコバクター・ピロリ / 萎縮性胃炎 / エピジェネティック / エピジェネティッ ク / DNAメチル化異常 / H. pylori |
|---|
| Outline of Final Research Achievements |
This study aimed to elucidate the correlation between altered mucosal DNA methylation levels and activity of H. pylori-related gastritis, because inflammatory activity are associated with the development of diffuse-type gastric cancer. Methylation levels (promoter CpG islands (FLNc,HAND1, THBD, p41ARC, HRASLS, and LOX) and the CpG sites of non-coding repetitive elements (Alu and Sata) were examined by real-time methylation-specific PCR or bisulfite pyrosequencing. Methylation levels of the six CpG islands were consistently increased, and those of the two repetitive elements were consistently decreased in a stepwise manner with the activity of gastric inflammation. The observed correlation between altered DNA methylation levels and activity of H. pylori-related gastritis appears to be one of the relevant molecular mechanisms underlying the development of diffuse-type cancer.
We might identify novel diffuse-type gastric cancer risk marker.
|
