Searching for neurodegenerative disease-related factors in the metabolic systems of ADMA-containing protein and the elucidation of the functional mechanism
| Project/Area Number |
23590683
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Okayama Prefectural University |
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Principal Investigator |
KIMOTO Masumi 岡山県立大学, 保健福祉学部, 教授 (40108866)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Yoshitaka 岡山県立大学, 保健福祉学部, 教授 (10236333)
YAMAMOTO Toshiko 岡山県立大学, 保健福祉学部, 准教授 (60301313)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Makiko 高知県立大学, 健康栄養学部, 准教授 (60437001)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 翻訳後修飾 / アルギニンメチル化タンパク質 / 中枢神経系 / ADMA / PRMT / DDAH / 一酸化窒素 / 非対称性ジメチルアルギニン / タンパク質の翻訳後修飾 / 内因性NOS阻害剤 / 老化 / NOS阻害剤 / RPS2 / COXII |
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| Research Abstract |
To demonstrate the physiological significance of the metabolic pathways for asymmetric dimethylarginine (ADMA) in central nervous system, we examined the immunohistochemical localization of ADMA metabolizing enzymes, PRMT1 and DDAH1 and nNOS in the rat spinal cord and the other brain regions. Western blot analyses showed that PRMT1 and DDAH1 were widely distributed. The immunohistochemical analyses showed that these enzymes colocalized in a lot of neurons. In the cortex and hypothalamus, almost all nNOS neurons had PRMT1 and DDAH1. Additionally, we surveyed unknown arginine methylated proteins from rat brain by proteomic analysis and identified fructose 1,6-bisphosphate aldolase C (FBAC) as a novel ADMA-containing protein. These findings suggest that the PRMT/DDAH/NOS axis is play a part of homeostatic function in central nervous system.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] The hydrolase DDAH2 enhances pancreatic insulin secretion by transcriptional regulation of secretagogin through a Sirt 1-dependent mechanism in mice2013
Author(s)
Hasegawa K, Wakino S, Kimoto M, Minakuchi H, Fujimura K, Komatsu M, Kaneko Y, Kanda T, Tokuyama H, Hayashi K, Itoh H
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Journal Title
FASEB J
Volume: 27
Pages: 2301-2315
Related Report
Peer Reviewed
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[Journal Article] The hydrolase DDAH2 enhances pancreatic insulin secretion by transcriptional regulation of secretagogin through a Sirt1-dependent mechanism in mice2013
Author(s)
Hasegawa K, Wakino S, Kimoto M, Minakuchi H, Fujimura K, Hosoya K, Komatsu M, Kaneko Y, Kanda T, Tokuyama H, Hayashi K, Itoh H
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Journal Title
FASEB J
Volume: 27
Issue: 6
Pages: 2301-2315
DOI
Related Report
Peer Reviewed
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[Journal Article] Nuetralization of leukotriene C4 and D4 activity by monoclonal and single-chain antibodies.2013
Author(s)
Kawakami Y, Hirano S, Kinoshita M, Otsuki A, Suzuki-Yamamoto T, Suzuki M, Kimoto M, Sasabe S, Fukushima M, Kishimoto K, Izumi T, Oga T, Narumiya S, Sugahara M, Miyano M, Yamamoto S, Takahashi Y.
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Journal Title
Biochim. Biophys. Acta
Volume: 1840
Pages: 1625-1633
Related Report
Peer Reviewed
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[Journal Article] Gastrointestinal digestion and absorption of Pen j 1, a major allergen from kuruma prawn, Penaeus japonicus.2011
Author(s)
Kunimoto A, Yokoro M, Murota K, Yamanishi R, Suzuki-Yamamoto T, Suzuki M, Yutani C, Doi S, Hiemori M, Yamashita H, Takahashi Y, Tsuji H, and Kimoto M.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 75
Issue: 7
Pages: 1249-1258
NAID
Related Report
Peer Reviewed
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