Analysis of the expression of antigens including carbohydrate chains and the association of the antigen expression between cell function and disease

• Project/Area Number: 23590686
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Jichi Medical University
• Principal Investigator: MUROI KAZUO 自治医科大学, 医学部, 教授 (50190939)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Chizuru 自治医科大学, 医学部, 助教 (60306149) ; FUJIWARA Shinichiro 自治医科大学, 医学部, 講師 (20438667)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 抗原 / 細胞分化 / 悪性リンパ腫 / 骨髄異形成症候群 / 抗原発現 / 慢性骨髄性増殖性疾患 / CD25 / 芽球 / 造血回復

Research Abstract

The positivity of CD25 in B cell lymphomas derived from pregerminal center and postgerminal center were higher than that derived from germinal center. The prognosis of CD25+ diffuse large B-cell lymphoma (DLBCL) and CD25+ follicular lymphoma (FL) were worse than that of CD25- DLBCL and CD25- FL. No difference between gene expression in CD25+ B-cell lymphoma cells and CD25- B-cell lymphoma cells was shown. Decrease of B lymphoblasts in the bone marrow blast region was shown to predict progression of refractory anemia of myleodysplastic syndrome. Granulocytes in a recovery state after hemopoietic stem cell transplantation were shown to express CD56. CD10 expression was observed in granulocytes of a patient with adult-onset chronic granulomatous disease.

Research Products

• [Journal Article] Clinical features of de novo CD25-positive follicular lymphoma. (2014)
  • Author(s): Fujiwara S, Muroi K, Tatara R, Matsuyama T, Ohmine K, Suzuki T, Mori M, Nagai T, Tanaka A, Ozawa K.
  • Journal Title: Leuk Lymphoma. Volume: 55 Issue: 2 Pages: 307-313
  • DOI: 10.3109/10428194.2013.806658
  • Peer Reviewed
• [Journal Article] CD56 Expression in Normal Immature Granulocytes after Allogeneic Hematopoietic Stem Cell Transplantation (2013)
  • Author(s): Muroi K, Fujiwara S, Tatara R, Sugimoto M, Yamamoto C, Uehara E, Meguro A, Hatano K, Okazuka K, Oh I, Ohmine K, Suzuki T, Mori M, Nagai T, Ozawa K.
  • Journal Title: Journal of Clinical and Experimental Hematopathology Volume: 53 Issue: 3 Pages: 247-250
  • DOI: 10.3960/jslrt.53.247
  • NAID: 130003384001
  • ISSN: 1346-4280, 1880-9952
  • Peer Reviewed
• [Journal Article] Unrelated allogeneic bone marrow-derived mesenchymal stem cells for steroid-refractory acute graft-versus-host disease: a phase I/II study. (2013)
  • Author(s): Muroi K, Miyamura K, Ohashi K, Murata M, Eto T, Kobayashi N, Taniguchi S, Imamura M, Ando K, Kato S, Mori T, Teshima T, Mori M, Ozawa K.
  • Journal Title: Int J Hematol. Volume: 98 Issue: 2 Pages: 206-213
  • DOI: 10.1007/s12185-013-1399-4
  • NAID: 10031194736
  • Peer Reviewed
• [Journal Article] Analysis of hemolysis in collected bone marrow for bone marrow transplantation. (2013)
  • Author(s): Sugimoto M, Fujiwara S, Hosonuma R, Matsu H, Uehara E, Yamamoto C, Kobayashi H, Hatano K, Meguro A, Tatara R, Okabe H, Oh I, Matsuyama T, Ohmine K, Suzuki T, Mori M, Nagai T, Ozawa K, Muroi K.
  • Journal Title: Transfus Apher Sci. Volume: 49 Issue: 1 Pages: 95-96
  • DOI: 10.1016/j.transci.2013.02.045
  • Peer Reviewed
• [Journal Article] Clinical features of de novo CD25(+) diffuse large B-cell lymphoma. (2013)
  • Author(s): Fujiwara S, Muroi K, et al.
  • Journal Title: Hematology. Volume: 18巻 Issue: 1 Pages: 14-19
  • DOI: 10.1179/1607845412y.0000000024
  • Peer Reviewed
• [Journal Article] Adult-Onset Chronic Granulomatous Disease and CD10-Negativity in Neutrophils (2012)
  • Author(s): Yamamoto C, Muroi K, Okabe H, Uehara E, Hirano T, Sugiyama Y, Ozawa K
  • Journal Title: Journal of Clinical and Experimental Hematopathology Volume: 52 Issue: 3 Pages: 219-221
  • DOI: 10.3960/jslrt.52.219
  • NAID: 10031143567
  • ISSN: 1346-4280, 1880-9952
  • Peer Reviewed
• [Journal Article] Flow Cytometric Analysis of Kappa and Lambda Light Chain Expression in Endoscopic Biopsy Specimens before the Diagnosis of B-Cell Lymphoma (2012)
  • Author(s): Oka S, et al.
  • Journal Title: Journal of Clinical and Experimental Hematopathology Volume: 52 Issue: 2 Pages: 127-131
  • DOI: 10.3960/jslrt.52.127
  • NAID: 10031120437
  • ISSN: 1346-4280, 1880-9952
  • Peer Reviewed
• [Journal Article] Autologous hematopoietic recovery with aberrant antigen expression after allogeneic bone marrow transplantation. (2012)
  • Author(s): Kobayashi H
  • Journal Title: J Clin Exp Hematop. Volume: 52 Issue: 4 Pages: 81-83
  • DOI: 10.3109/10428194.2012.725850
  • Peer Reviewed
• [Journal Article] Autologous Hematopoietic Recovery with Aberrant Antigen Expression after Allogeneic Bone Marrow Transplantation (2012)
  • Author(s): Kobayashi, H., Nagai, T., Muroi, K.
  • Journal Title: Journal of Clinical and Experimental Hematopathology Volume: 52 Issue: 1 Pages: 81-83
  • DOI: 10.3960/jslrt.52.81
  • NAID: 10030615702
  • ISSN: 1346-4280, 1880-9952
  • Peer Reviewed
• [Journal Article] Prediction of Progression from Refractory Cytopenia with Unilineage Dysplasia by Analysis of Bone Marrow Blast Cell Composition (2012)
  • Author(s): Oka S, Muroi K, Fujiwara S, Oh I, Matsuyama T, Ohmine K, Suzuki T, Ozaki K, Mori M, Nagai T, Ozawa K, Hanafusa T
  • Journal Title: Journal of Clinical and Experimental Hematopathology Volume: 52 Issue: 1 Pages: 63-66
  • DOI: 10.3960/jslrt.52.63
  • NAID: 10030615661
  • ISSN: 1346-4280, 1880-9952
  • Peer Reviewed
• [Journal Article] Prediction of progression from refractory cytopenia with unilineage dysplasia by analysis of bone marrow blast cell composition. (2012)
  • Author(s): Oka S, Muroi K, Fujiwara S, et al.
  • Journal Title: J Clin Exp Hematop. Volume: 52巻 Pages: 63-66
  • NAID: 10030615661
  • Peer Reviewed
• [Journal Article] Adult-onset chronic granulomatous disease and CD10-negativity in neutrophils. (2012)
  • Author(s): Yamamoto C, Muroi K, et al.
  • Journal Title: J Clin Exp Hematop. Volume: 52巻 Pages: 219-221
  • Peer Reviewed
• [Journal Article] Use of cryoprotectant-depleted allogeneic peripheral blood stem cells for transplantation (2011)
  • Author(s): Hirata Y, Kishino K, Onozaki F, Nakaki Y, Fujiwara S, Yamamoto C, Sato K, Matsuyama T, Ozaki K, Mori M, Ozawa K, Muroi K
  • Journal Title: Hematology Volume: 16 Issue: 4 Pages: 221-224
  • DOI: 10.1179/102453311x13025568941664
  • Peer Reviewed
• [Presentation] Profiles Of De Novo CD25-Positive Mature B-Cell Lymphomas (2013)
  • Author(s): Fujiwara S, Muroi k, 他8名
  • Organizer: 2013 Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2013-12-09
• [Presentation] Prognostic significance of CD11b expression in de novo diffuse large B-cell lymphoma (2013)
  • Author(s): Fujiwara S, Muroi k, 他9名
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2013-10-12
• [Presentation] Third-party derived mesenchymal stem cells for steroid-resistant acute GVHD : a multicenter phase I/II study (2013)
  • Author(s): Muroi K
  • Organizer: AsiaCord 2013
  • Place of Presentation: Kobe
  • Year and Date: 2013-04-20
• [Presentation] Clinical Features of Newly Diagnosed CD25-Positive Follicular Lymphoma (2012)
  • Author(s): Fujiwara S, Muroi K, 他7名
  • Organizer: 2012 Annual Meeting of the American Society of Hematology
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2012-12-08
• [Presentation] Clinical features of de novo CD25-positive diffuse large B-cell lymphoma (2011)
  • Author(s): Fujiwara S, Muroi K, 他9名
  • Organizer: 2011 Annual Meeting of the American Society of Hematology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-12-11
• [Presentation] Clinical Features of De Novo CD25-Positive Diffuse Large B-Cell Lymphoma (2011)
  • Author(s): Kazuo Muroi
  • Organizer: American Society of Hematology Annual Meeting
  • Place of Presentation: San Diego
• [Presentation] Profiles Of De Novo CD25-Positive Mature B-Cell Lymphomas.
  • Author(s): Fujiwara S, Muroi k, 他8名
  • Organizer: 2013 Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans, USA.
• [Presentation] Third-party derived mesenchymal stem cells for steroid-resistant acute GVHD: a multicenter phase I/II study.
  • Author(s): Muroi K.
  • Organizer: AsiaCord 2013
  • Place of Presentation: 神戸
• [Presentation] Prognostic significance of CD11b expression in de novo diffuse large B-cell lymphoma.
  • Author(s): Fujiwara S, Muroi k, 他9名.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌
• [Presentation] Clinical features of de novo CD25(+) diffuse large B-cell lymphoma.
  • Author(s): Fujiwara S, Muroi K, et al.
  • Organizer: 日本血液学会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Clinical Features of Newly Diagnosed CD25-Positive Follicular Lymphoma
  • Author(s): Fujiwara S, Muroi K, et al.
  • Organizer: ASH Annual Meeting
  • Place of Presentation: Georgia World Congress Center(アトランタ）
• [Book] 血液・造血器疾患のマネジメント-血液内科医と病理医との対話— (2011)
  • Author(s): 室井一男, 藤原慎一郎
  • Total Pages: 219
  • Publisher: 医薬ジャーナル社
• [Book] 血液・造血器疾患のマネジメントー血液内科医と病理医の対話ー (2011)
  • Author(s): 室井一男
  • Total Pages: 219
  • Publisher: 医薬ジャーナル社