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HHV-6 species specific serological assay

Research Project

Project/Area Number 23590693
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionFujita Health University

Principal Investigator

HIGASHIMOTO Yuki  藤田保健衛生大学, 医学(系)研究科(研究院), 研究員 (20569701)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Etsushi  藤田保健衛生大学, 医学部, 教授 (80288472)
IHIRA Masaru  藤田保健衛生大学, 医療科学部, 教授 (10290165)
ENOMOTO Yoshihiko  藤田保健衛生大学, 医学系研究科, 研究員 (00387713)
SUGIYAMA Hiroko  藤田保健衛生大学, 医学系研究科, 研究員 (10387714)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords臨床検査医学 / 小児科学 / ウイルス学 / 病態検査学 / 国際情報交換 / 臨床検査学 / 米国 / ザンビア / 国際情報交流
Research Abstract

HHV-6 is divided into two species; HHV-6A and HHV-6B. Primary HHV-6B infection causes exanthem subitum (ES) that is common infectious disease in childhood. In contrast to HHV-6B, epidemiology and clinical feature of HHV-6A infection remains unclear because of no species-specific serological assay. To elucidate full-spectrum of HHV-6A infection, we sought to develop species-specific HHV-6 serological assay.
We have developed a new immunoblot assay that could discriminate between HHV-6A and B antibodies. However, this method is inappropriate for high-throughput analysis. Then, we tried to use synthesized peptides located within the target regions. Two peptide will be able to use ELISA for species-specific ELISA assays.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2012 2011

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (4 results)

  • [Journal Article] Development of human herpesvirus 6 variant specific immunoblotting assay2012

    • Author(s)
      Higashimoto Y, Ohta A, Nishiyama Y, Ihira M, Sugata K, Asano Y, Ablashi DV, Yoshikawa T
    • Journal Title

      J Clin Microbiol

      Volume: 50 Pages: 1245-51

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of real-time RT-PCR assays for detection of three classes of HHV-6 gene expressions2012

    • Author(s)
      Ihira M, Higashimoto Y, Sugiyama H, Enomoto Y, Kawamura Y, Nakai H, Sugata K, Asano Y, Yoshikawa T
    • Journal Title

      J Med Virol

      Volume: 84

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum biomarker kinetics with three different courses of HHV-6 encephalitis2012

    • Author(s)
      Kawamura Y, Nakai H, Sugata K, Yoshikawa T
    • Journal Title

      Brain Develop

      Volume: (pii : S0387-7604(12)00214-8)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a human herpesvirus 6 species-specific immunoblotting assay.2012

    • Author(s)
      Higashimoto Y, Ohta A, Nishiyama Y, Ihira M, Sugata K, Asano Y, Peterson DL, Ablashi DV, Lusso P, Yoshikawa T.
    • Journal Title

      Journal of clinical microbiology

      Volume: 50 Pages: 1245-1251

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Kinetics of the cytokines and chemokines in cases with primary HHV-6 infection2011

    • Author(s)
      Yoshikawa T, Sugata K, Asano Y, Ihira M, Kumagai T
    • Journal Title

      J Clin Virol

      Volume: 50 Pages: 65-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Different characteristics of human herpesvirus 6 encephalitis between primary infection and viral reactivation2011

    • Author(s)
      Kawamura Y, Sugata K, Ihira M, Mihara T, Mutoh T, Asano Y, Yoshikawa T
    • Journal Title

      J Clin Virol

      Volume: 51 Pages: 12-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Development of species-specific peptides ELISA assay for HHV-62012

    • Author(s)
      東本祐紀
    • Organizer
      IDWeek 2012
    • Place of Presentation
      San Deigo, California(poster発表)
    • Year and Date
      2012-10-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of species-specific peptides ELISA assay for HHV-62012

    • Author(s)
      Yuki Higashimoto
    • Organizer
      IDWeek: A Joint Meeting of IDSA, SHEA, HIVMA, and PIDS.
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Development of human herpesvirus 6 variant specific immunoblotting assay2011

    • Author(s)
      東本祐紀
    • Organizer
      IHW (International Herpesvirus Workshop) 2011
    • Place of Presentation
      Gdansk,poland(poster発表)
    • Year and Date
      2011-07-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of a human herpesvirus 6 species-specific immunoblotting assay.2011

    • Author(s)
      東本 祐紀
    • Organizer
      36th Annual International Herpesvirus Workshop
    • Place of Presentation
      Gdansk, Poland
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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