| Budget Amount *help |
¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Research Abstract |
There was no significant promoter activity stimulation by cAMP and PMA in any of three cell lines. However in HSKTC, cAMP increased Cables promoter activity about two fold higher than control and Forskolin increased Cables promoter activity about five fold higher than control. Forskolin is known to stimulate intracellular accumulation of cAMP and this in turn may be the cause of stimulation of Cables promoter activity in response to Forskolin in the HSKTC.
These experiments suggest that Cables promoter regulation mechanism depends on the cell type and may be dictated by differences in underlying epigenetic modulation of the Cables promoter. Ovarian carcinoma is difficult to detect by clinical laboratory test and often develops chemoresistance. Future experiments will continue to define the regulation of Cables 1 promoter, which may help predict better treatment or early diagnostics of ovarian carcinoma.
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