| Project/Area Number |
23590696
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
TOHYAMA Kaoru 川崎医科大学, 医学部, 教授 (80227561)
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| Co-Investigator(Renkei-kenkyūsha) |
TASAKA Taizo 川崎医科大学, 医学部, 准教授 (80343309)
TSUJIOKA Takayuki 川崎医科大学, 医学部, 講師 (50330551)
SUEMORI Shin-ichiro 川崎医科大学, 医学部, 講師 (60330552)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 遺伝子ノックダウン法 / 血球異形成 / 食細胞機能 / 好中球機能 |
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| Research Abstract |
I focused attention on vimentin, one of the intermediate filaments which support cell shape and might be involved in the mechanisms of hematopoietic dysplasia, and examined the change of vimentin expression during the differentiation process of human leukemic HL60 cell line. Vimentin expression was down-regulated in the process of neutrophil-like differentiation of HL60 cells, while it was extremely up-regulated in the process of macrophage-like differentiation.
Next, I established HL60 cell clones whose vimentin expression was knock-downed by transfer of a lentiviral vector harboring vimentin-shRNA. Such vimentin-knock-downed clones were induced to differentiation and revealed increased leukocyte activity such as cell movement and phagocytosis. These data suggested that vimentin regulates leukocyte function negatively.
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