Approach to the molecular mechanisms of hematopoietic dysplasia aiming at elucidation of the pathogenesis of myelodysplastic syndromes
| Project/Area Number |
23590696
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
TOHYAMA Kaoru 川崎医科大学, 医学部, 教授 (80227561)
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| Co-Investigator(Renkei-kenkyūsha) |
TASAKA Taizo 川崎医科大学, 医学部, 准教授 (80343309)
TSUJIOKA Takayuki 川崎医科大学, 医学部, 講師 (50330551)
SUEMORI Shin-ichiro 川崎医科大学, 医学部, 講師 (60330552)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 遺伝子ノックダウン法 / 血球異形成 / 食細胞機能 / 好中球機能 |
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| Research Abstract |
I focused attention on vimentin, one of the intermediate filaments which support cell shape and might be involved in the mechanisms of hematopoietic dysplasia, and examined the change of vimentin expression during the differentiation process of human leukemic HL60 cell line. Vimentin expression was down-regulated in the process of neutrophil-like differentiation of HL60 cells, while it was extremely up-regulated in the process of macrophage-like differentiation.
Next, I established HL60 cell clones whose vimentin expression was knock-downed by transfer of a lentiviral vector harboring vimentin-shRNA. Such vimentin-knock-downed clones were induced to differentiation and revealed increased leukocyte activity such as cell movement and phagocytosis. These data suggested that vimentin regulates leukocyte function negatively.
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Report
(4 results)
Research Products
(90 results)
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[Journal Article] Morphologic analysis in myelodysplastic syndromes with del(5q) treated with lenalidomide. A Japanese multiinstitutional study2012
Author(s)
Matsuda A, Taniwaki M, Jinnai I, Harada H, Watanabe M, Suzuki K, Yanagita S, Suzuki T, Yoshida Y, Kimura A, Tsudo M, Tohyama K, Takatoku M, Ozawa K
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Journal Title
Leuk Res
Volume: (in press)
Issue: 5
Pages: 575-580
DOI
Related Report
Peer Reviewed
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[Journal Article] Clinical utility of the neutrophil distribution pattern obtained using the CELL-DYN SAPPHIRE hematology analyzer for the diagnosis of myelodysplastic syndrome2011
Author(s)
Inaba T, Yuki Y, Yuasa S, Fujita N, Yoshitomi K, Kamisako T, Torii K, Okada T, Urasaki Y, Ueda T, Tohyama K
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Journal Title
Int J Hematol
Volume: 94
Issue: 2
Pages: 169-77
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Genome-wide identification of human microRNAs located in leukemia-associated genomic alterations2011
Author(s)
Starczynowski DT, Morin R, McPherson A, Lam J, Chari R, Wegrzyn J, Kuchenbauer F, Hirst M, Tohyama K, Humphries RK, Lam WL, Marra M, Karsan A
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Journal Title
Blood
Volume: 117
Issue: 2
Pages: 595-607
DOI
Related Report
Peer Reviewed
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[Journal Article] 骨髄増殖性腫瘍におけるJAK2-V617F変異遺伝子比率と好中球アルカリホスファターゼスコアとの関連2011
Author(s)
奥野菜々子, 近藤敏範, 岸本光代, 辻岡貴之, 末盛晋一郎, 松岡亮仁, 久山亜紀, 田坂大象, 大倉貢, 野田幸代, 通山薫
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Journal Title
日本検査血液学会雑誌
Volume: 12
Pages: 170-176
Related Report
Peer Reviewed
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[Presentation] MDSの診断と治療2014
Author(s)
通山薫
Organizer
日本血液学会中四国地方会・教育セミナー
Place of Presentation
徳島市(ザ・グランドパレス徳島)
Year and Date
2014-02-28
Related Report
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[Presentation] MDSの形態診断
Author(s)
通山 薫
Organizer
第12回自治医大クリニカルヘマトロジーセミナー
Place of Presentation
自治医科大学（栃木）
Related Report
Invited
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[Presentation] MDSの診断と治療
Author(s)
通山 薫
Organizer
日本血液学会中四国地方会・教育セミナー
Place of Presentation
ザ・グランドパレス徳島（徳島）
Related Report
Invited
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[Presentation] MDS診療の展望
Author(s)
通山 薫
Organizer
第2回MDS・分子標的療法研究会
Place of Presentation
フォーシーズンズホテル（東京）
Related Report
Invited
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[Presentation] MDSの最新情報
Author(s)
通山 薫
Organizer
第3回香川血液疾患フォーラム
Place of Presentation
JRホテルクレメント高松（高松）
Related Report
Invited
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[Presentation] MDSの形態診断
Author(s)
通山 薫
Organizer
第3回神奈川MDS研究会
Place of Presentation
ホテルキャメロットジャパン（横浜）
Related Report
Invited
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