| Project/Area Number |
23590697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUENAGA Midori 徳島文理大学, 薬学部, 助教 (00389181)
坪井 義夫 福岡大学, 医学部, 教授 (90291822)
上原 吉就 福岡大学, 医学部, 講師 (70373149)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUBOI Yoshio 福岡大学, 医学部, 教授 (90291822)
UEHARA Yoshinari 福岡大学, 医学部, 講師 (70373149)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | アルツハイマー病 / アミロイドベータ蛋白 / ベータシート構造 / オリゴマー形成 / 蛋白凝集 / ビタミンＤ２ / ビタミンＤ３ / プリオン蛋白 / ベータシート形成 / 脳内蛋白異常凝集 / アルツハイマー病診断 / アルツハイマー病患者血清 / 蛋白加温修飾 / アミロイドベータ蛋白特異抗体 |
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| Research Abstract |
Aggregated amyloid-beta is a pathological feature in Alzheimer's disease(AD) and mild cognitive impairment(MCI) is an early stage of AD. Up to date, there is no diagnostics of MCI by patients serum. We found that the concentration of 25(OH)V-D3 in serum with MCI is lower than that of normal volunteers, and there is no differences in the concentration of 1,25(OH)2V-D3 between them. We suggest that the serum concentration of 25(OH)V-D3 is useful for the diagnostics of MCI. Unexpectedly, 25(OH)V-D3 showed no inhibitory effects for the aggregation in vitro, and rather promoted the aggregation.
PrPsc is the responsible protein to cause scrapie, which derived from the conformational transition of PrPc in normal brain. Oligomerization of PrPc is a pre-stage of PrPsc. We found that V-D3 could not break the oligomerization, however VD2 could break the oligzation, suggesting that VD2 is useful for scrapie therapy.
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