Research of established monoclonal antibodies against tumor related molecules in a pancreatic juice

• Project/Area Number: 23590698
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Fukuoka University
• Principal Investigator: NAKASHIMA manabu 福岡大学, 薬学部, 教授 (50198074)
• Research Collaborator: YANO Hirohisa 久留米大学, 医学部, 教授 ; OHSHIMA Kouichi 久留米大学, 医学部, 教授 ; YASUMOTO Makiko 久留米大学, 医学部・大学院 ; NISHINAKAGAWA Takuya 福岡大学, 薬学部, 助教 ; HAMABASHIRI Masato 福岡大学, 大学院 ; DAICHO Aya 福岡大学, 大学院
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 免疫学 / 癌マーカー / 単クローナル抗体 / 膵癌 / 膵液 / Tn 抗原 / シアリル化ルイス a 抗原 / 腫瘍検査学 / 単クローン抗体

Outline of Final Research Achievements

We have established mouse monoclonal antibodies (2P-1-2-1, 2P-1-17-1, 6P-3-2-4) against malignant tumor related molecules in a pancreatic juice. The molecules recognized by these monoclonal antibodies were studied. Because 2P-1-17-1 and 6P-3-2-4 have shown immune-reactivity against glycol-antigen resulted by western-blotting analysis, synthetic glycol-pepetides were used as antigens for ELISA and Westernblotting assay. As a consequence of these assay, 2P-1-17-1 and 6P-3-2-4 reacted with sialyl lews a and Tn antigen, respectively. 2P-1-2-1 recognized the molecule which was 100~130 kDa in molecular size with a fine band on westernblotting. The molecule was purified by immune-precipitation method using protein G coated magnetic beads and isolated by SDS-PAGE gel electrophoresis. Isolated molecule in SDS-PAGE gel was analysed by LC-MS/MS, resulting that molecule was a bile salt-dependent lipase oncofetal isoform, which was specifically produced in pancreas acinal cell, with sore 828.

Research Products

• [Journal Article] The utility of a novel antibody in the pathological diagnosis of pancreatic acinar cell carcinoma (2012)
  • Author(s): M Yasumoto,M Hamabashiri,J Akiba,S Ogasawara,Y Naito,T Taira,M Nakayama,A Daicho,F Yamasaki,K Shimamatsu,Y Ishida,R Kaji,Y Okabe,O Nakashima,K Ohshima,M Nakashima,M Sata,H Yano
  • Journal Title: J Clin Pathol. Volume: 65 Pages: 327-332
  • Peer Reviewed
• [Journal Article] Novel monoclonal antibodies against pancreatic juice from pancreatic cancer patients and their possible application in differential diagnosis. (2011)
  • Author(s): Masato Hamabashiri, Aya Daichou, Makiko Yasumoto, Sachiko Ogasawara,Takuya Nishinakagawa, Munechika Enjoji, Kouichi Ohshima, Hirohisa Yano and Manabu Nakashima
  • Journal Title: Int J Mol Med. Volume: 28 Pages: 599-603
  • NAID: 110009552409
  • Peer Reviewed
• [Presentation] Tn抗原に対する新規モノクローナル抗体6P-3-2-4と22-1-1との反応性の比較 (2012)
  • Author(s): 濱走優人、桑水流裕太、安元真希子、秋葉 純、小笠原幸子、西中川拓也、矢野博久、中島 学
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌（北海道大学）
• [Presentation] A novel monoclonal antibody against Tn antigen and comparison of reactivity with 22-1-1 (2011)
  • Author(s): M. Hamabashiri, M. Yasumoto, T. Nishinakagawa, S. Ogasawara, M. Enjoji, K. Ohshima,H. Yano, M. Nakashima
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋（名古屋国際会議場）