Role of GPR103-26RFa-QRFP system in the development of neuropathic pain

• Project/Area Number: 23590715
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pain science
• Research Institution: Kumamoto University
• Principal Investigator: TANAHIRA Chiyoko 熊本大学, 生命科学研究部, 助教 (20555776)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Tatsuo 熊本大学, 大学院生命科学研究部, 教授 (20200818)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: GPR108 / orphan receptor / 神経障害性疼痛 / 炎症性疼痛 / 可塑性 / 神経科学 / 神経伝達物質 / 痛み / GPR103 / 26RFa / QRFP / ノックアウトマウス

Research Abstract

In the present study, the effects of intrathecal and intracerebroventricular application of 26RFa, on the level of mechanical allodynia induced by partial sciatic nerve ligation, were examined in rats. Intrathecal and intracerebroventricular injection of 26RFa attenuated the level of mechanical allodynia in a dose dependent manner. Immunohistochemical examination revealed that QRFP-like immunoreactivity (QRFP-LI) was expressed mainly in the small to medium sized neuronal profiles in the L5 dorsal root ganglion and that partial sciatic nerve injury increased the percentage of QRFP-LI positive neuronal profiles and made the cross-section area of the QRFP-LI positive neuronal profiles larger in the L5 dorsal root ganglion ipsilateral to the nerve injury.
Microinjection of 5 g of 26RFa into RVM decreased the number of flinching behavior induced by paw formalin injection significantly as compared with saline injected rats. This effect of 26RFa was not antagonized by naloxone.

Research Products

• [Journal Article] Anti-allodynic effects of intrathecally and intracerebro- ventricularly administered 26RFa, an intrinsic agonist for GRP103, in the rat partial sciatic nerve ligation model (2011)
  • Author(s): Yamamoto T, Miyazaki R, Yamada T, Shinozaki T
  • Journal Title: Peptides Volume: 32 Pages: 1262-1269
  • Peer Reviewed
• [Presentation] Microinjection of Neuropeptide W, an endogeniou ligand for GPR7 and 8, into rostral ventromedial medulla (RVM) produces an analgesic effect in the rat formalin test (2014)
  • Author(s): Takahiro Nonaka, Toshihiko Yamada, Miki Araki, Tatsuo Yamamoto
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington DC,アメリカ
• [Presentation] Microinjection of 26RFa, an endogeniou ligand for GPR103, into rostral ventromedial medulla (RVM) produces an analgesic effect in the rat formalin test (2014)
  • Author(s): Miki Araki, Takahiro Nonaka, Toshihiko Yamada, Tatsuo Yamamoto
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington DC,アメリカ
• [Presentation] Microinjection of 26RFa, an endogenous ligand for GPR103, into rostralventromedial medulla (RVM) produces an analgesic effect in the rat formalin test (2014)
  • Author(s): Tatsuo Yamamoto
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington DC
• [Presentation] Microinjection of Neuropeptide W, an endogenous ligand for GPR7 and 8, into rostral ventromedial medulla (RVM) produces an analgesic effect in the rat formalin test. (2014)
  • Author(s): Tatsuo Yamamoto
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington DC