Pain regulation by PKA and calcineurin through functional modulation of T-type calcium channels
| Project/Area Number |
23590729
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Pain science
|
|---|
| Research Institution | Kinki University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SEKIGUCHI Fumiko 近畿大学, 薬学部, 准教授 (90271410)
TSUBOTA Maho 近畿大学, 薬学部, 助教 (90510123)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | カルシウムチャネル / ＰＫＡ / カルシニューリン / 痛み / 神経突起伸長 / 薬理学 / PKA / 神経突起 / イオンチャネル / カルシウム / プロテインキナーゼA / 疼痛制御 |
|---|
| Research Abstract |
Among three isoforms of T-type calcium channels, Cav3.2 expressed abundantly in the peripheral ending of nociceptors plays a critical role in nociceptive processing. We thus examined molecular mechanisms for functional regulation of Cav3.2 by phosphorylation and dephosphorylation, and its impact on pain signals. Our data show that PKA, activated by stimulation of prostaglandin EP4 receptors in an AKAP150-dependent manner, phosphorylates and functionally upregulates Cav3.2, leading to hyperalgesia, and that calcineurin, a phosphatase, negatively regulates Cav3.2 functions.
|
Report
(4 results)
Research Products
(35 results)
-
-
[Journal Article] AKAP-dependent sensitization of Cav3.2 channels via the EP4 receptor/cyclic AMP pathway mediates prostaglandin E2-induced mechanical hyperalgesia.2013
Author(s)
Sekiguchi, F., Matsumoto, Y., Maeda, Y., Tsubota-Matsunami, M., Nishikawa, H. and Kawabata, A.
-
Journal Title
Br. J. Pharmacol.
Volume: 168
Issue: 3
Pages: 734-745
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Prostaglandin E_2-induced sensitization of T-type Ca^<2+> channels : a mechanism for mechanical hyperalgesia2012
Author(s)
Kanaoka, D., Sekiguchi, F., Nakagawa, M., Aoki Y., Nishimoto Y., Yoshida, S., Tsubota-Matsunami, M, Kawabata, A
Organizer
A Satellite Symposium of IUPHAR-GI Section, International Ulcer Week 2012
Place of Presentation
東京
Year and Date
2012-07-12
Related Report
-
-
-
[Presentation] Molecular mechanisms for the neurite outgrowth caused by prostaglandin E_2 in mouse dorsal root ganglion cells2012
Author(s)
Maeda, T., Mitani, K., Yamagata, R., Sekiguchi, F., Kawabata, A
Organizer
Neuroscience 2012
Place of Presentation
New Orleans, USA
Related Report
-
[Presentation] Roles of endogenous hydrogen sulfide in the cyclic AMP-induced neuronal differentiation in NG108-15 cells2012
Author(s)
Kanaoka, D., Takeda, Y., Sekiguchi, F., Yoshida, S., Kawabata, A
Organizer
Neuroscience 2012
Place of Presentation
New Orleans, USA
Related Report
-
-
-
-
-
[Presentation] AKAP-dependent modulation of phosphorylation levels of Ca_v3.2 T-type calcium channels by protein kinase A and calcineurin : implications for mechanical pain sensitivity2011
Author(s)
Sekiguchi, F., Kanaoka, D., Nakagawa, M., Aoki, Y., Nishimoto, Y., Yoshida, S., Matsunami, M., Kawabata, A
Organizer
Neuroscience 2011
Place of Presentation
Washington, DC
Related Report
-
[Presentation] Sensitization of T-type calcium channels by prostaglandin E_2 via the EP4 receptor/cyclic AMP/protein kinase A pathway : a possible mechanism for mechanical hyperalgesia2011
Author(s)
Kanaoka, D., Sekiguchi, F., Nakagawa, M., Aoki, Y., Nishimoto, Y., Yoshida, S., Matsunami, M., Kawabata, A
Organizer
Neuroscience 2011
Place of Presentation
Washington, DC
Related Report
-
-
-
[Presentation] Sensitization of T-type calcium channels by prostaglandin E2 via the EP4 receptor/cyclic AMP/protein kinase A pathway: a possible mechanism for mechanical hyperalgesia.2011
Author(s)
Kanaoka, D., Sekiguchi, F., Nakagawa, M., Aoki, Y., Nishimoto, Y., Yoshida, S., Matsunami, M., Kawabata, A.
Organizer
Neuroscience 2011
Place of Presentation
Washington DC, USA
Related Report
-
[Presentation] AKAP-dependent modulation of phosphorylation levels of Cav3.2 T-type calcium channels by protein kinase A and calcineurin: implications for mechanical pain sensitivity2011
Author(s)
Sekiguchi, F., Kanaoka, D., Nakagawa, M., Aoki, Y., Nishimoto, Y., Yoshida, S., Matsunami, M., Kawabata, A.
Organizer
Neuroscience 2011
Place of Presentation
Washington DC, USA
Related Report
-
-
-
-
[Presentation] Prostaglandin E2-induced sensitization of T-type Ca2+ channels: a mechanism for mechanical hyperalgesia.
Author(s)
Kanaoka, D., Sekiguchi, F., Nakagawa, M., Aoki Y., Nishimoto Y., Yoshida, S., Tsubota-Matsunami, M, Kawabata, A.
Organizer
A Satellite Symposium of IUPHAR-GI Section, International Ulcer Week 2012
Place of Presentation
京王プラザホテル東京（東京都）
Related Report
-
-
-
