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Inhibitory modulation of the pain sensor protein, TRPA1

Research Project

Project/Area Number 23590730
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pain science
Research InstitutionHyogo University of Health Sciences

Principal Investigator

DAI YI  兵庫医療大学, 薬学部, 准教授 (20330441)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsTRPA1 / TRPV1 / artemin / resveratrol / etodolac / TRPチャネル / DRG / 疼痛 / レスベラトロール / Ret / GFRα3
Research Abstract

The transient receptor potential (TRP) channel subtype V1 (TRPV1) and A1 (TRPA1) are known to be expressed on sensory neurons and respond to changes in temperature, pH and local application of certain noxious chemicals. In this study, we investigated mechanisms of inhibitory modulation of these TRP channels. Following is the major findings of this study.
1. We found that artemin or resveratrol can inhibit TRPA1. The mechanism is likely due to a decreasing of the channel density of TRPA1 on cell membranes. 2. Pinosylvin methyl ether can inhibit TRPV1 by changing the EC50, but not the maximum response of capsaicin-induced currents. 3. Etodolac can direct activate and subsequently desensitize TRPA1 through a covalent modification of cysteine residues. These data indicate that the inhibitory mechanism of modulation of TRP channels are various and complicated.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (36 results)

All 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 8 results) Presentation (24 results)

  • [Journal Article] 痛みの受容器2014

    • Author(s)
      戴 毅、野口光一
    • Journal Title

      日本医師会雑誌(特別号「痛みのマネジメント update-基礎知識から緩和ケアまで」)

      Volume: 143

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Etodolac activates and desensitizes transient receptor potential ankyrin 12013

    • Author(s)
      Wang S, Dai Y, Kogure Y, Yamamoto S, Zhang W, Noguchi K
    • Journal Title

      J Neurosci Res

      Volume: 91(12) Issue: 12 Pages: 1591-8

    • DOI

      10.1002/jnr.23274

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of TRP channels by resveratrol and other stilbenoids.2013

    • Author(s)
      Yu L, Wang S, Kogure Y, Yamamoto S, Noguchi K, Dai Y.
    • Journal Title

      Mol Pain.

      Volume: Vol.9 Pages: 3-13

    • DOI

      10.1186/1744-8069-9-3

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Potentiation of the P2X3 ATP receptor by PAR-2 in rat DRG neurons, through protein kinase-dependent mechanisms, contributes to inflammatory pain2012

    • Author(s)
      Wang S, Dai Y, Kobayashi K, Zhu W, Kogure Y, Yamanaka H, Wan Y, Zhang W, Noguchi K.
    • Journal Title

      Eur J Neurosci

      Volume: 36 Issue: 3 Pages: 2293-301

    • DOI

      10.1111/j.1460-9568.2012.08142.x

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Up-regulation of platelet-activating factor synthases and its receptor in spinal cord contribute to development of neuropathic pain following peripheral nerve injury.2012

    • Author(s)
      Okubo M, Yamanaka H, Kobayashi K, Kanda H, Dai Y, Noguchi K.
    • Journal Title

      Mol Pain

      Volume: 8 Pages: 8-8

    • DOI

      10.1186/1744-8069-8-8

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Re-evaluation of the phenotypic changes in L4 dorsal root ganglion neurons after L5 spinal nerve ligation2012

    • Author(s)
      Fukuoka T, Yamanaka H, Kobayashi K, Okubo M, Miyoshi K, Dai Y, Noguchi K
    • Journal Title

      Pain

      Volume: 153(1) Pages: 68-79

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Re-evaluation of the phenotypic changes in L4 dorsal root ganglion neurons after L5 spinal nerve ligation.2012

    • Author(s)
      Fukuoka T, et al.
    • Journal Title

      PAIN

      Volume: 153 Pages: 68-79

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 痛みとペインセンサーTRPA1の役割2011

    • Author(s)
      戴毅
    • Journal Title

      Clinical Neuroscience(臨床神経科学)

      Volume: Vol.29 (10) Pages: 1201-1203

    • Related Report
      2013 Final Research Report
  • [Journal Article] 東洋医学からみた痛みの制御2011

    • Author(s)
      戴毅
    • Journal Title

      脳21

      Volume: 14(1) Pages: 61-67

    • Related Report
      2013 Final Research Report
  • [Journal Article] Inhibition of TRPA1 channel activity in sensory neurons by the glial cell line-derived neurotrophic factor family member, artemin.2011

    • Author(s)
      Yoshida N, Kobayashi K, Yu L, Wang S, Na R, Yamamoto S, Noguchi K, Dai Y.
    • Journal Title

      Mol Pain

      Volume: 7 Pages: 41-41

    • DOI

      10.1186/1744-8069-7-41

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of TRPA1channel activity in sensory neurons by the glial cell line-derived neurotrophic factor family member, artemin.2011

    • Author(s)
      Yoshida N, et al.
    • Journal Title

      Mollecular Pain

      Volume: 7 Pages: 41-41

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 痛みとペインセンサーTRPA1の役割2011

    • Author(s)
      戴 毅
    • Journal Title

      臨床神経科学

      Volume: 29 Pages: 1201-1203

    • Related Report
      2011 Research-status Report
  • [Presentation] Etodolac activates and desensitizes TRPA12013

    • Author(s)
      王勝蘭, 戴毅, 野口光一
    • Organizer
      第6回日本運動器疼痛学会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2013-12-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Expression of colony-stimulating factors and their receptors in dorsal root ganglion and spinal cord after peripheral nerve injury2013

    • Author(s)
      OKUBO M, YAMANAKA H, KOBAYASHI K, KANDA H, DAI Y, NOGUCHI K
    • Organizer
      43^<rd> annual meeting of neuroscience 2013
    • Place of Presentation
      San Diego Covention Center : Sails Parilion (San Diego, USA)
    • Year and Date
      2013-11-09
    • Related Report
      2013 Final Research Report
  • [Presentation] レスベラトロールと他のスチルベン化合物によるTRPチャネル修飾2013

    • Author(s)
      戴毅, 于麗娜, 王勝蘭, 野口光一
    • Organizer
      第35回日本疼痛学会
    • Place of Presentation
      大宮ソニックシティ(埼玉県さいたま市)
    • Year and Date
      2013-07-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 虎杖の主成分resveratrolによる疼痛関連受容体TRPA1チャネルの活性抑制2013

    • Author(s)
      戴毅
    • Organizer
      第64回日本東洋医学会学術総会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2013-06-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Etodolac activates and desensitizes transient receptor potential ankyrin 1 through cysteine modification2013

    • Author(s)
      Wang Shenglan, Noguchi Koichi, Dai Yi
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of TRPV1 and TRPA1 channels expression in sensory neurons by artemin after inflammation2012

    • Author(s)
      MIYAGAWA Y, KOBAYASHI K, YAMANAKA H, YU L, DAI Y, YAGI H, NOGUCHI K
    • Organizer
      42^<th> annual meeting of neuroscience 2012
    • Place of Presentation
      Moial Convention Center (New Orleans, USA)
    • Year and Date
      2012-10-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Inhibitory effect of stilbenoids on TRP channels2012

    • Author(s)
      Yu L, Dai Y, Noguchi K
    • Organizer
      42^<th> annual meeting of neuroscience 2012
    • Place of Presentation
      Moial Convention Center (New Orleans, USA)
    • Year and Date
      2012-10-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Delayed intrathecal infusion of glial cell line-derived neurotrophic factor exerts anti-allodynic effect by mitigation of injury-induced neuropeptide expression in touch-sense pathway following l5 spinal nerve ligation2012

    • Author(s)
      Fukuoka T, Dai Y, Noguchi K
    • Organizer
      14^<th> world congress on pain
    • Place of Presentation
      Milano Convention Centre (Milano, Italy)
    • Year and Date
      2012-08-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Arteminによる疼痛関連TRP受容体発現調2012

    • Author(s)
      宮川慈子, 小林希実子, 山中博樹, 戴毅, 八木秀司, 野口光一
    • Organizer
      第34回日本疼痛学会
    • Place of Presentation
      熊本市国際交流会館(熊本市)
    • Year and Date
      2012-07-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Evodiamine activates TRPV1 in primary sensory neuron2012

    • Author(s)
      岩岡恵実子, 松吉延之, 小暮洋子, 青木俊二, 山本悟史, 野口光一, 戴毅
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2012-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Delayed intrathecal infusion of glial cell line-derived neurotrophic factor exerts anti-allodynic effect by mitigation of injury-induced neuropeptide expression in touch-sense pathway following l5 spinal nerve ligation2012

    • Author(s)
      Fukuoka T, Dai Y, Noguchi K
    • Organizer
      14th world congress on pain
    • Place of Presentation
      Milano Convention Centre (Milano, Italy)
    • Related Report
      2012 Research-status Report
  • [Presentation] Inhibitory effect of stilbenoids on TRP channels2012

    • Author(s)
      Yu L, Dai Y, Noguchi K
    • Organizer
      42th annual meeting of neuroscience 2012
    • Place of Presentation
      Moial Convention Center (New Orleans, USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of TRPV1 and TRPA1 channels expression in sensory neurons by artemin after inflammation2012

    • Author(s)
      MIYAGAWA Y, KOBAYASHI K, YAMANAKA H, YU L, DAI Y, YAGI H, NOGUCHI K
    • Organizer
      42th annual meeting of neuroscience 2012, New Orleans, 2012. 10. 15
    • Place of Presentation
      Moial Convention Center (New Orleans, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Arteminによる疼痛関連TRP受容体発現調節2012

    • Author(s)
      宮川慈子、小林希実子、山中博樹、戴 毅、八木秀司、野口光一
    • Organizer
      第34回日本疼痛学会
    • Place of Presentation
      熊本市国際交流会館(熊本市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Evodiamine activates TRPV1 in primary sensory neurons2012

    • Author(s)
      岩岡恵実子, et al
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Pinosylvin methyl ether desensitizes the TRPV1 channel2011

    • Author(s)
      Yu L, Noguchi K. Dai Y
    • Organizer
      41^<th> annual meeting of neuroscience 2011
    • Place of Presentation
      Walter E. Washington Convention Center (Washington D.C., USA)
    • Year and Date
      2011-11-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Short-term treatment of artemin functionally inhibits TRPA1 channel activity in rat primary sensory neurons2011

    • Author(s)
      Yoshida N, Kobayashi K, Na R, Yu Lina, Noguchi K, Dai Y
    • Organizer
      The 4^<th> Asian Pain Symposium
    • Place of Presentation
      320 YUE YANG ROAD (SHANGHAI , China)
    • Year and Date
      2011-05-16
    • Related Report
      2013 Final Research Report
  • [Presentation] ゴシュユの主成分エボジアミンによる一次知覚ニューロンの活性化2011

    • Author(s)
      岩岡恵実子, 松吉延之, 小暮洋子, 青木俊二, 野口光一, 戴毅
    • Organizer
      第28回和漢医薬学会学術大会
    • Place of Presentation
      富山県民会館(富山市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Short-term treatment of artemin functionally inhibits TRPA1 channel activity in rat primary sensory neurons2011

    • Author(s)
      Yoshida N, et al.
    • Organizer
      The 4th Asian Pain Symposium
    • Place of Presentation
      320 YUE YANG ROAD, SHANGHAI, China
    • Related Report
      2011 Research-status Report
  • [Presentation] ゴシュユの主成分エボジアミンによる一次知覚ニューロンの活性化2011

    • Author(s)
      岩岡恵実子, et al.
    • Organizer
      第28回和漢医薬学会
    • Place of Presentation
      富山県県民会館(富山)
    • Related Report
      2011 Research-status Report
  • [Presentation] Pinosylvin methyl ether desensitizes the TRPV1 channel.2011

    • Author(s)
      Lina Yu, et al
    • Organizer
      41st annual meeting of neuroscience 2011
    • Place of Presentation
      Walter E. Washington Convention Center. (Washington DC, USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] 虎杖の主成分resveratrolによる疼痛関連受容体TRPA1チャネルの活性抑制

    • Author(s)
      戴 毅
    • Organizer
      第64回日本東洋医学会学術総会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] レスベラトロールと他のスチルベン化合物によるTRPチャネル修飾

    • Author(s)
      戴 毅, 于 麗娜, 王 勝蘭, 野口 光一
    • Organizer
      第35回日本疼痛学会
    • Place of Presentation
      大宮ソニックシティ(埼玉県さいたま市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Etodolac activates and desensitizes TRPA1

    • Author(s)
      王 勝蘭、戴 毅、野口 光一
    • Organizer
      第6回日本運動器疼痛学会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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