Identification and molecular epidemiological analysis of emerging clones of community-acquired MRSA

• Project/Area Number: 23590746
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene
• Research Institution: Sapporo Medical University
• Principal Investigator: KOBAYASHI Nobumichi 札幌医科大学, 医学部, 教授 (80186759)
• Co-Investigator(Kenkyū-buntansha): SUMI Ayako 札幌医科大学, 医学部, 准教授 (10363699) ; URUSHIBARA Noriko 札幌医科大学, 医学部, 講師 (80396308) ; GHOSH Souvik 札幌医科大学, 医学部, 助教 (30597175)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: MRSA / 市中感染 / 分子疫学 / PVL / USA300 / 国際研究者交流 / バングラデシュ / ミャンマー

Research Abstract

To investigate prevalence in Japan of community-acquired MRSA (CA-MRSA) which is concerned for its global spread, and their molecular epidemiological characteristics, we analyzed clinical isolates from the Sapporo Medical University Hospital (601 isolates, 2008-2010) and those from local hospitals/clinics in Hokkaido prefecture (outpatients, 422 isolates in 2011). As a result, SCCmec types IV and V, which are genetic markers of CA-MRSA, were detected in 4.3% and 17.1% in the isolates from the University Hospital and local hospitals/clinics, respectively. Among them, seven isolates were revealed to be USA300 clone which is dominant in the US (ST8, positive for PVL and ACME), and many ST5 MRSA having ACME were detected. These emerging CA-MRSA clones were confirmed to be distributed in Japan.

Research Products

• [Journal Article] Detection and Genetic Characterization of PVL-Positive ST8-MRSA-IVa and Exfoliative Toxin D-Positive European CA-MRSA-Like ST1931 (CC80) MRSA-IVa Strains in Bangladesh (2014)
  • Author(s): Paul SK, Ghosh S, Kawaguchiya M, Urushibara N, Hossain MA, Ahmed S, Mahmud C, Jilani MS, Haq JA, Ahmed AA, Kobayashi N
  • Journal Title: Microb Drug Resist Volume: 19
  • Peer Reviewed
• [Journal Article] Characterization of PVL/ACME-positive methicillin- resistant Staphylococcus aureus (genotypes ST8-MRSA-IV and ST5-MRSA-II) isolated from a university hospital in Japan (2013)
  • Author(s): Kawaguchiya M, Urushibara N, Yamamoto D, Yamashita T, Shinagawa M, Watanabe N, Kobayashi N
  • Journal Title: Microb Drug Resist Volume: 19 Issue: 1 Pages: 48-56
  • DOI: 10.1089/mdr.2012.0089
  • Peer Reviewed
• [Journal Article] Characterization of Enterococcus faecium with macrolide resistance and reduced susceptibility to quinupristin/dalfopristin in a Japanese hospital : detection of extensive diversity in erm(B)- regulator regions (2013)
  • Author(s): Isogai N, Urushibara N, Kawaguchiya M, Ghosh S, Suzaki K, Watanabe N, Quiñones D, Kobayashi N
  • Journal Title: Microb Drug Resist Volume: 19 Issue: 4 Pages: 298-307
  • DOI: 10.1089/mdr.2012.0176
  • Peer Reviewed
• [Journal Article] Effect of temperature, relative humidity, and rainfall on rotavirus infections in Kolkata, India (2013)
  • Author(s): Sumi A, Rajendran K, Ramamurthy T, Krishnan T, Nair GB, Harigane K, Kobayashi N
  • Journal Title: Epidemiol Infect Volume: 141 Issue: 8 Pages: 1652-1661
  • DOI: 10.1017/s0950268812002208
  • Peer Reviewed
• [Journal Article] Genetic diversity of emerging PVL/ACME-positive ST8-MRSA- IVa and ACME-positive CC5 (ST5/ST764)-MRSA strains in northern Japan (2013)
  • Author(s): Kawaguchiya M, Urushibara N, Ghosh S, Kuwahara O, Morimoto S, Ito M, Kudo K, Kobayashi N
  • Journal Title: J Med Microbiol Volume: 62 Issue: 12 Pages: 1852-1863
  • DOI: 10.1099/jmm.0.062125-0
  • Peer Reviewed
• [Journal Article] Characterization of Enterococcus faecium with Macrolide Resistance and Reduced Susceptibility to Quinupristin/Dalfopristin in a Japanese Hospital: Detection of Extensive Diversity in erm(B)-Regulator Regions. (2013)
  • Author(s): Isogai N, Urushibara N, Kawaguchiya M, Ghosh S, Suzaki K, Watanabe N, Quiñones D, Kobayashi N.
  • Journal Title: Microbial Drug Resistance Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Analysis of staphylococcal cassette chromosome mec (SCCmec) in Staphylococcus haemolyticus and Staphylococcus sciuri: identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7). (2011)
  • Author(s): Urushibara N, Paul SK, Hossain MA, Kawaguchiya M, Kobayashi N.
  • Journal Title: Microbial Drug Resistance Volume: 17 Pages: 291-297
  • Peer Reviewed
• [Journal Article] Virulence factors and genetic characteristics of Methicillin-resistant and -susceptible Staphylococcus aureus isolates in Myanmar. (2011)
  • Author(s): Aung MS, Urushibara N, Kawaguchiya M, Aung TS, Mya S, San T, Nwe KM, Kobayashi N.
  • Journal Title: Microbial Drug Resistance Volume: 17 Pages: 525-535
  • Peer Reviewed
• [Presentation] Novel multiplex PCR in combination with mutagenic PCR-RFLP analysis to distinguish Streptococcus pneumoniae serotypes 6A, 6B, 6C, and 6D (2014)
  • Author(s): Kawaguchiya M, Urushibara N, Kobayashi
  • Organizer: The 12^<th> Japan-Korea International Symposium on Microbiology
  • Place of Presentation: Tokyo
  • Year and Date: 2014-03-25
• [Presentation] Two novel staphylococcal cassette chromosome mec elements containing two copies of mecA genes identified in Bangladeshi clinical isolates of Staphylococcus haemolyticus (2013)
  • Author(s): Urushibara N, Kawaguchiya M, Paul SK, Kobayashi N
  • Organizer: International Interscience Conference on Infection and Chemotherapy
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2013-11-08
• [Presentation] Serotype distribution and analysis of macrolide- and penicillin-resistance among non-invasive S. pneumoniae isolates in northern Japan (2013)
  • Author(s): Kawaguchiya M, Urushibara N, Ghosh S, Ito M, Kudo K, Kuwahara O, Kobayashi N
  • Organizer: The 28^<th> International Congress of Chemotherapy and Infection
  • Place of Presentation: Yokohama
  • Year and Date: 2013-07-07
• [Presentation] Characterization and genetic diversity of PVL/ACME- positive MRSA strains isolated in Hokkaido, the northern main island of Japan (2013)
  • Author(s): Kobayashi N, Kawaguchiya M, Urushibara N, Ghosh S, Ito M, Kudo K, Kuwahara O, Quiñones D
  • Organizer: The 28th International Congress of Chemotherapy and Infection
  • Place of Presentation: Yokohama
  • Year and Date: 2013-06-06
• [Presentation] Prevalence and genetic characterization of SCCmec and ACME in coagulase-negative staphylococci (2013)
  • Author(s): Onishi M, Urushibara N, Shinagawa M, Watanabe N, Kobayashi N
  • Organizer: The 28^<th> International Congress of Chemotherapy and Infection
  • Place of Presentation: Yokohama
  • Year and Date: 2013-06-06
• [Presentation] Structural variations of ACME-SCCmec composite islands in MRSA carrying SCCmec type II collected in Hokkaido, Japan (2013)
  • Author(s): Urushibara N, Kawaguchiya M, Kobayashi
  • Organizer: The 28^<th> International Congress of Chemotherapy and Infection
  • Place of Presentation: Yokohama
  • Year and Date: 2013-06-06
• [Presentation] Characterization and genetic diversity of PVL/ACME-positive MRSA strains isolated in Hokkaido, the northern main island of Japan. (2013)
  • Author(s): Kobayashi N, Kawaguchiya M, Urushibara N, Ghosh S, Ito M, Kudo K, Kuwahara O, Quiñones D
  • Organizer: The 28th International Congress of Chemotherapy and Infection
  • Place of Presentation: 横浜
• [Presentation] Prevalence and genetic characterization of SCCmec and ACME in coagulase-negative staphylococci. (2013)
  • Author(s): Onishi M, Urushibara N, Shinagawa M, Watanabe N, Kobayashi N
  • Organizer: 28th International Congress of Chemotherapy and Infection
  • Place of Presentation: 横浜
• [Presentation] Structural variations of ACME-SCCmec composite islands in MRSA carrying SCCmec type II collected in Hokkaido, Japan. (2013)
  • Author(s): Urushibara N, Kawaguchiya M, Kobayashi N
  • Organizer: 28th International Congress of Chemotherapy and Infection
  • Place of Presentation: 横浜
• [Presentation] Analysis of macrolide-lincosamide-streptogramin B resistance in Enterococcus faecium isolated in a Japanese hospital (2012)
  • Author(s): Kobayashi N, Isogai N, Urushibara N, Watanabe N
  • Organizer: The 13th Asia-Pacific congress of Clinical Microbiology and Infection
  • Place of Presentation: Beijing
  • Year and Date: 2012-10-26
• [Presentation] Genetic diversities in ACME-SCCmec composite islands in MRSA isolates collected in Hokkaido, the northern main island of Japan (2012)
  • Author(s): Urushibara N, Kawaguchiya M, Kobayashi
  • Organizer: 15th International Symposium on Staphylococci and Staphylococcal Infections
  • Place of Presentation: Lyon, France
• [Presentation] Analysis of macrolide-lincosamide-streptogramin B resistance in Enterococcus faecium isolated in a Japanese hospital. (2012)
  • Author(s): Kobayashi N, Isogai N, Urushibara N, Watanabe N
  • Organizer: The 13th Asia-Pacific congress of Clinical Microbiology and Infection
  • Place of Presentation: 北京
• [Presentation] Genetic diversities in ACME-SCCmec composite islands in MRSA isolates collected in Hokkaido, the northern main island of Japan (2012)
  • Author(s): Urushibara N, Kawaguchiya M, Kobayashi N
  • Organizer: 15th International Symposium on Staphylococci and Staphylococcal Infections
  • Place of Presentation: リヨン
• [Presentation] Genetic characterization of SCCmec type IV MRSA isolated from skin infections in Bangladesh. (2012)
  • Author(s): 川口谷充代、漆原範子、パウル シャマル、小林宣道
  • Organizer: 第85 回日本細菌学会総会
  • Place of Presentation: 長崎市
• [Presentation] Genetic diversities in ACME-SCCmec composite islands in CA-MRSA isolates collected in Hokkaido. (2012)
  • Author(s): 漆原範子、川口谷充代、小林宣道
  • Organizer: 第85 回日本細菌学会総会
  • Place of Presentation: 長崎市
• [Presentation] Genetic analysis of PVL/ACME- positive Staphylococcus aureus isolated in a Japanese hospital (2011)
  • Author(s): Kawaguchiya M, Urushibara N, Yamamoto D, Shinagawa M, Watanabe N, Kobayashi
  • Organizer: 13th International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo
• [Presentation] Prevalence and genetic mechanisms of macrolide-lincosamide -streptogramin B resistance in Enterococcus faecium isolated in a Japanese hospital (2011)
  • Author(s): Isogai N, Urushibara N, Quinones D, Kobayashi N
  • Organizer: 13th International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo
• [Presentation] Genetic analysis of PVL/ACME-positive Staphylococcus aureus isolated in a Japanese hospital. (2011)
  • Author(s): Kawaguchiya M, Urushibara N, Yamamoto D, Shinagawa M, Watanabe N, Kobayashi N.
  • Organizer: 13th International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: 札幌市
• [Presentation] Characterization of two novel arginine catabolic mobile element (ACME) and staphylococcal chromosome cassette mec (SCCmec) composite islands found in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). (2011)
  • Author(s): Urushibara N, Kawaguchiya M, Kobayashi N.
  • Organizer: 13th International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: 札幌市