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Identification and molecular epidemiological analysis of emerging clones of community-acquired MRSA

Research Project

Project/Area Number 23590746
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hygiene
Research InstitutionSapporo Medical University

Principal Investigator

KOBAYASHI Nobumichi  札幌医科大学, 医学部, 教授 (80186759)

Co-Investigator(Kenkyū-buntansha) SUMI Ayako  札幌医科大学, 医学部, 准教授 (10363699)
URUSHIBARA Noriko  札幌医科大学, 医学部, 講師 (80396308)
GHOSH Souvik  札幌医科大学, 医学部, 助教 (30597175)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsMRSA / 市中感染 / 分子疫学 / PVL / USA300 / 国際研究者交流 / バングラデシュ / ミャンマー
Research Abstract

To investigate prevalence in Japan of community-acquired MRSA (CA-MRSA) which is concerned for its global spread, and their molecular epidemiological characteristics, we analyzed clinical isolates from the Sapporo Medical University Hospital (601 isolates, 2008-2010) and those from local hospitals/clinics in Hokkaido prefecture (outpatients, 422 isolates in 2011). As a result, SCCmec types IV and V, which are genetic markers of CA-MRSA, were detected in 4.3% and 17.1% in the isolates from the University Hospital and local hospitals/clinics, respectively. Among them, seven isolates were revealed to be USA300 clone which is dominant in the US (ST8, positive for PVL and ACME), and many ST5 MRSA having ACME were detected. These emerging CA-MRSA clones were confirmed to be distributed in Japan.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (19 results)

  • [Journal Article] Detection and Genetic Characterization of PVL-Positive ST8-MRSA-IVa and Exfoliative Toxin D-Positive European CA-MRSA-Like ST1931 (CC80) MRSA-IVa Strains in Bangladesh2014

    • Author(s)
      Paul SK, Ghosh S, Kawaguchiya M, Urushibara N, Hossain MA, Ahmed S, Mahmud C, Jilani MS, Haq JA, Ahmed AA, Kobayashi N
    • Journal Title

      Microb Drug Resist

      Volume: 19

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of PVL/ACME-positive methicillin- resistant Staphylococcus aureus (genotypes ST8-MRSA-IV and ST5-MRSA-II) isolated from a university hospital in Japan2013

    • Author(s)
      Kawaguchiya M, Urushibara N, Yamamoto D, Yamashita T, Shinagawa M, Watanabe N, Kobayashi N
    • Journal Title

      Microb Drug Resist

      Volume: 19 Issue: 1 Pages: 48-56

    • DOI

      10.1089/mdr.2012.0089

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of Enterococcus faecium with macrolide resistance and reduced susceptibility to quinupristin/dalfopristin in a Japanese hospital : detection of extensive diversity in erm(B)- regulator regions2013

    • Author(s)
      Isogai N, Urushibara N, Kawaguchiya M, Ghosh S, Suzaki K, Watanabe N, Quiñones D, Kobayashi N
    • Journal Title

      Microb Drug Resist

      Volume: 19 Issue: 4 Pages: 298-307

    • DOI

      10.1089/mdr.2012.0176

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of temperature, relative humidity, and rainfall on rotavirus infections in Kolkata, India2013

    • Author(s)
      Sumi A, Rajendran K, Ramamurthy T, Krishnan T, Nair GB, Harigane K, Kobayashi N
    • Journal Title

      Epidemiol Infect

      Volume: 141 Issue: 8 Pages: 1652-1661

    • DOI

      10.1017/s0950268812002208

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity of emerging PVL/ACME-positive ST8-MRSA- IVa and ACME-positive CC5 (ST5/ST764)-MRSA strains in northern Japan2013

    • Author(s)
      Kawaguchiya M, Urushibara N, Ghosh S, Kuwahara O, Morimoto S, Ito M, Kudo K, Kobayashi N
    • Journal Title

      J Med Microbiol

      Volume: 62 Issue: 12 Pages: 1852-1863

    • DOI

      10.1099/jmm.0.062125-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Enterococcus faecium with Macrolide Resistance and Reduced Susceptibility to Quinupristin/Dalfopristin in a Japanese Hospital: Detection of Extensive Diversity in erm(B)-Regulator Regions.2013

    • Author(s)
      Isogai N, Urushibara N, Kawaguchiya M, Ghosh S, Suzaki K, Watanabe N, Quiñones D, Kobayashi N.
    • Journal Title

      Microbial Drug Resistance

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of staphylococcal cassette chromosome mec (SCCmec) in Staphylococcus haemolyticus and Staphylococcus sciuri: identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7).2011

    • Author(s)
      Urushibara N, Paul SK, Hossain MA, Kawaguchiya M, Kobayashi N.
    • Journal Title

      Microbial Drug Resistance

      Volume: 17 Pages: 291-297

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Virulence factors and genetic characteristics of Methicillin-resistant and -susceptible Staphylococcus aureus isolates in Myanmar.2011

    • Author(s)
      Aung MS, Urushibara N, Kawaguchiya M, Aung TS, Mya S, San T, Nwe KM, Kobayashi N.
    • Journal Title

      Microbial Drug Resistance

      Volume: 17 Pages: 525-535

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Novel multiplex PCR in combination with mutagenic PCR-RFLP analysis to distinguish Streptococcus pneumoniae serotypes 6A, 6B, 6C, and 6D2014

    • Author(s)
      Kawaguchiya M, Urushibara N, Kobayashi
    • Organizer
      The 12^<th> Japan-Korea International Symposium on Microbiology
    • Place of Presentation
      Tokyo
    • Year and Date
      2014-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Two novel staphylococcal cassette chromosome mec elements containing two copies of mecA genes identified in Bangladeshi clinical isolates of Staphylococcus haemolyticus2013

    • Author(s)
      Urushibara N, Kawaguchiya M, Paul SK, Kobayashi N
    • Organizer
      International Interscience Conference on Infection and Chemotherapy
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2013-11-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Serotype distribution and analysis of macrolide- and penicillin-resistance among non-invasive S. pneumoniae isolates in northern Japan2013

    • Author(s)
      Kawaguchiya M, Urushibara N, Ghosh S, Ito M, Kudo K, Kuwahara O, Kobayashi N
    • Organizer
      The 28^<th> International Congress of Chemotherapy and Infection
    • Place of Presentation
      Yokohama
    • Year and Date
      2013-07-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization and genetic diversity of PVL/ACME- positive MRSA strains isolated in Hokkaido, the northern main island of Japan2013

    • Author(s)
      Kobayashi N, Kawaguchiya M, Urushibara N, Ghosh S, Ito M, Kudo K, Kuwahara O, Quiñones D
    • Organizer
      The 28th International Congress of Chemotherapy and Infection
    • Place of Presentation
      Yokohama
    • Year and Date
      2013-06-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Prevalence and genetic characterization of SCCmec and ACME in coagulase-negative staphylococci2013

    • Author(s)
      Onishi M, Urushibara N, Shinagawa M, Watanabe N, Kobayashi N
    • Organizer
      The 28^<th> International Congress of Chemotherapy and Infection
    • Place of Presentation
      Yokohama
    • Year and Date
      2013-06-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural variations of ACME-SCCmec composite islands in MRSA carrying SCCmec type II collected in Hokkaido, Japan2013

    • Author(s)
      Urushibara N, Kawaguchiya M, Kobayashi
    • Organizer
      The 28^<th> International Congress of Chemotherapy and Infection
    • Place of Presentation
      Yokohama
    • Year and Date
      2013-06-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization and genetic diversity of PVL/ACME-positive MRSA strains isolated in Hokkaido, the northern main island of Japan.2013

    • Author(s)
      Kobayashi N, Kawaguchiya M, Urushibara N, Ghosh S, Ito M, Kudo K, Kuwahara O, Quiñones D
    • Organizer
      The 28th International Congress of Chemotherapy and Infection
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Prevalence and genetic characterization of SCCmec and ACME in coagulase-negative staphylococci.2013

    • Author(s)
      Onishi M, Urushibara N, Shinagawa M, Watanabe N, Kobayashi N
    • Organizer
      28th International Congress of Chemotherapy and Infection
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structural variations of ACME-SCCmec composite islands in MRSA carrying SCCmec type II collected in Hokkaido, Japan.2013

    • Author(s)
      Urushibara N, Kawaguchiya M, Kobayashi N
    • Organizer
      28th International Congress of Chemotherapy and Infection
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analysis of macrolide-lincosamide-streptogramin B resistance in Enterococcus faecium isolated in a Japanese hospital2012

    • Author(s)
      Kobayashi N, Isogai N, Urushibara N, Watanabe N
    • Organizer
      The 13th Asia-Pacific congress of Clinical Microbiology and Infection
    • Place of Presentation
      Beijing
    • Year and Date
      2012-10-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic diversities in ACME-SCCmec composite islands in MRSA isolates collected in Hokkaido, the northern main island of Japan2012

    • Author(s)
      Urushibara N, Kawaguchiya M, Kobayashi
    • Organizer
      15th International Symposium on Staphylococci and Staphylococcal Infections
    • Place of Presentation
      Lyon, France
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of macrolide-lincosamide-streptogramin B resistance in Enterococcus faecium isolated in a Japanese hospital.2012

    • Author(s)
      Kobayashi N, Isogai N, Urushibara N, Watanabe N
    • Organizer
      The 13th Asia-Pacific congress of Clinical Microbiology and Infection
    • Place of Presentation
      北京
    • Related Report
      2012 Research-status Report
  • [Presentation] Genetic diversities in ACME-SCCmec composite islands in MRSA isolates collected in Hokkaido, the northern main island of Japan2012

    • Author(s)
      Urushibara N, Kawaguchiya M, Kobayashi N
    • Organizer
      15th International Symposium on Staphylococci and Staphylococcal Infections
    • Place of Presentation
      リヨン
    • Related Report
      2012 Research-status Report
  • [Presentation] Genetic characterization of SCCmec type IV MRSA isolated from skin infections in Bangladesh.2012

    • Author(s)
      川口谷充代、漆原範子、パウル シャマル、小林宣道
    • Organizer
      第85 回日本細菌学会総会
    • Place of Presentation
      長崎市
    • Related Report
      2011 Research-status Report
  • [Presentation] Genetic diversities in ACME-SCCmec composite islands in CA-MRSA isolates collected in Hokkaido.2012

    • Author(s)
      漆原範子、川口谷充代、小林宣道
    • Organizer
      第85 回日本細菌学会総会
    • Place of Presentation
      長崎市
    • Related Report
      2011 Research-status Report
  • [Presentation] Genetic analysis of PVL/ACME- positive Staphylococcus aureus isolated in a Japanese hospital2011

    • Author(s)
      Kawaguchiya M, Urushibara N, Yamamoto D, Shinagawa M, Watanabe N, Kobayashi
    • Organizer
      13th International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Prevalence and genetic mechanisms of macrolide-lincosamide -streptogramin B resistance in Enterococcus faecium isolated in a Japanese hospital2011

    • Author(s)
      Isogai N, Urushibara N, Quinones D, Kobayashi N
    • Organizer
      13th International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic analysis of PVL/ACME-positive Staphylococcus aureus isolated in a Japanese hospital.2011

    • Author(s)
      Kawaguchiya M, Urushibara N, Yamamoto D, Shinagawa M, Watanabe N, Kobayashi N.
    • Organizer
      13th International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      札幌市
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterization of two novel arginine catabolic mobile element (ACME) and staphylococcal chromosome cassette mec (SCCmec) composite islands found in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA).2011

    • Author(s)
      Urushibara N, Kawaguchiya M, Kobayashi N.
    • Organizer
      13th International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      札幌市
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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