SEARCHING OF SPECIFIC PROTEINS PRODUCED IN GALLBLADDER CANCER TISSUES FOR THE EARLY DETECTION OF THE CANCER

Research Project

Project/Area Number	23590759
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Hygiene
Research Institution	Niigata University of Health and Welfare
Principal Investigator	TSUCHIYA Yasuo 新潟医療福祉大学, 医療技術学部, 教授 (60334679)
Co-Investigator(Kenkyū-buntansha)	YAMAMOTO Masaharu 新潟医療福祉大学, 健康栄養学科, 教授 (40018693) YAMAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737) WAKAI Toshihumi 新潟大学, 医歯学系, 教授 (50372470)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000) Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords	胆嚢がん / スクリーニング / 特異蛋白 / 質量分析 / 腫瘍マーカー / 特異蛋白質 / 国際情報交換 / チリ / プロテオミクス解析
Research Abstract	Our operating procedure to detect and identify the specific proteins as the biomarker for the early detection of gallbladder cancer was as follows: preparation of paraffin sections from formalin-fixed paraffin-embedded gallbladder cancer tissue blocks, deparaffinization, stain sections on a microscope by Periodic acid-Schiff stain, excision of tissue section by laser microdissection, on-site direct digestion, purification of the samples using the C18 Stage Tip, concentration measurement of the peptide solutions by micro bicinchoninic acid protein assay, LC/MS/MS analysis. A total of 15 samples were collected: 3 from normal cell (group I), 6 from cancer cell (group II), and 6 from advanced cancer cell (group III). The average numbers of detected proteins from each group were 993, 1,291, and 1,109. In these proteins, the group specific proteins were 306, 1,148, and 930. The new biomarkers for the early detection of gallbladder cancer may be found to examine these proteins closely.