| Project/Area Number |
23590766
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | National Institute of Occupational Safety and Health, Japan |
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Principal Investigator |
WANG Rui-Sheng 独立行政法人労働安全衛生総合研究所, 健康障害予防研究グループ, 上席研究員 (10321895)
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| Co-Investigator(Kenkyū-buntansha) |
SUDA Megumi 独立行政法人 労働安全衛生総合研究所 (90415969)
WENG Zuquan 独立行政法人 労働安全衛生総合研究所 (10541170)
YANAGIBA Yukie 独立行政法人 労働安全衛生総合研究所 (90467283)
SUZUKI Tetsuya 独立行政法人 労働安全衛生総合研究所 (20573950)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ETBE / 遺伝毒性 / 代謝酵素 / 遺伝子多型 / 国際情報交換 / バイオ燃料 / 毒性評価 / ALDH2 / 感受性 |
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| Research Abstract |
Ethyl tertiary-butyl ether (ETBE) is a biofuel for vehicles. ETBE had toxicity only at high concentrations as showed in earlier experiments. However, it is not known if it induces damages when its metabolizing enzyme ALDH2 (aldehyde dehydrogenase 2) is inactive. Mice with normal, low active or inactive ALDH2 were exposed to ETBE at low concentrations (50-500 ppm). DNA damages in liver and sperm were observed in mice with low active and inactive ALDH2 exposed to 200 and 500 ppm ETBE, but not in mice with normal enzyme activity. Metabolites in blood and urine of mice exposed to ETBE or its intermediate metabolite TBA (tertiary-butyl alcohol) were analyzed and it was shown that ALDH2 is involved in the metabolism and toxicity of TBA, too. Our findings suggested that only exposure to high concentrations of ETBE may result in DNA damages in mice with active ALDH2, while low active and inactive ALDH2 enzyme can significantly enhance ETBE-induced damage even at low concentrations.
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