| Project/Area Number |
23590799
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Kyushu University |
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Principal Investigator |
FUJIMOTO Shuji 九州大学, 医学(系)研究科(研究院), 教授 (30199369)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Fumiko 九州大学, 大学院医学研究院, 講師 (80136564)
重松 美加 国立感染症研究所, その他部局等, 研究員 (20299598)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIGEMAYSU Mika 国立感染症研究所, 感染症疫学センター, 主任研究官 (20299598)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | Campylobacter enteritis / Food poisoning / Molecular epidemiology / カンピロバクター腸炎 / 食中毒 / 分子疫学 |
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| Research Abstract |
Campylobacter is one of the most common causes of bacterial gastroenteritis in Japan. The public health center in Fukuoka city, conducted the inspections of the restaurant and identified an association between eating several dishes containing chicken. We investigated the cases those had been identified with culture positive for Campylobacter. PCR using species-specific primer sets for Campylobacter showed that 10 strains isolated from the cases were C. jejuni. Restriction Fragment Length Polymorphic (RFLP) Analysis using PCR amplified flagellin gene demonstrated that two distinct patterns were observed among the strains. Random Amplified Polymorphic DNA (RAPD) analysis also showed the same result. Cytolethal distending toxin gene sequencing and Multilocus Sequence Typing analysis of the strains revealed that the two strains are not related each other. These data suggested that the outbreak might be caused by the food which was contaminated with the two distinct C. jejuni strains.
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