| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Research Abstract |
The problem with which forensic scientists have recently been confronted involves the technical difficulty of analyzing degraded DNA samples. We designed minimultiplex PCR systems using single-base extension reactions to identify Japanese Y chromosome haplogroups and mitochondrial DNA haplogroups. We selected a group of 22 Y chromosome single nucleotide polymorphisms(SNPs) and 46 mitochondrial DNA SNPs, respectively. We designed primers to render amplicons of <150 bp. Applying these systems, we classified the Japanese population into haplogroups and confirmed the applicability of these system in forensic degraded DNA analysis.
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