The role of dsRNA sensor in dendritic cells in a tolerized model of Type 1 diabetes
| Project/Area Number |
23590880
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
横野 浩一 神戸大学, その他部局等, その他 (50144580)
原 賢太 神戸大学, 医学部附属病院, 講師 (70397826)
明嵜 太一 神戸大学, 医学部附属病院, その他 (80467662)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | １型糖尿病 / 免疫寛容 / 病原体感知センサー / 樹状細胞 / 1型糖尿病 |
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| Research Abstract |
We found that administration of anti-Fas Ab completely suppressed diabetic onset in NOD mice as a type 1 diabetic mouse model. In addition, administration of bone marrow derived dendritic cells from Fas-deficient NOD-lpr mice which is diabetes-free also comletely protected from diabetes onset in NOD mice. To evaluate the role of TLR3 as dsRNA sensor of dendritic cells, administration n of poly (I:C) as TLR3 agonist in NOD mice was performed firstly. Low dose of poly(I:C) suppressed diabetes onset in NOD mice, whereas high dose of poly(I:C) accelerated diabetes onset in NOD mice. Next, to evaluate the role of TLR3 in a tolerized mouse model of type 1 diabetes, administartion of high dose of poly(I:C) was performed in tolerized mice. Surprisingly, we found that high dose of poly(I:C) induced diabetes and broke tolerance in tolerized NOD mice treated with anti-Fas Ab. We demonstrated the importance of dsRNA sensor in the development of type 1 diabetes.
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Report
(4 results)
Research Products
(29 results)
