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The evaluation of genomic aging by quantifying telomeric circular DNA in peripheral leukocytes of patients with chronic disease.

Research Project

Project/Area Number 23590885
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General internal medicine (including Psychosomatic medicine)
Research InstitutionKyushu University

Principal Investigator

MAEDA Toyoki  九州大学, 大学病院, 准教授 (30264112)

Co-Investigator(Kenkyū-buntansha) MAKINO Naoki  九州大学病院, 別府病院, 教授 (60157170)
KOYANAGI Masamichi  九州大学病院, 別府病院, 助教 (00325474)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords老化 / テロメア / 環状DNA / 白血球 / 血管内皮細胞
Research Abstract

This study aims to measure the aging promotion due to diseases by means of the somatic telomere length. Telomere is a structure of chromosome ends, which shortens at every cell cycle parallel to cell aging. The telomere length distribution is a parameter corresponding more sensitively to experimental stress or disease conditions than the mean telomere length or the amount of deleted telomeric DNA indicating the shortness of telomere. Hydrogen peroxide, life-style associated diseases, and chronic inflammation were associated with the increase of short telomeres, and the radiation exposure, chronic neurological diseases were associated with the decrease of short telomeres, which implied the increased death of old somatic cells. Taken together, there are two types of patterns of cellular aging in terms of the difference in the telomere length distribution.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (53 results)

All 2014 2013 2012 2011 Other

All Journal Article (39 results) (of which Peer Reviewed: 9 results) Presentation (12 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Gender and telomere length : systematic review and meta-analysis2014

    • Author(s)
      Gardner M, Bann D, Wiley L, Cooper R, Hardy R, Nitsch D, Martin-Ruiz C, Shiels P, Sayer AA, Barbieri M, Bekaert S, Bischoff C, Brooks-Wilson A, Chen W, Cooper C, Christensen K, De Meyer T, Deary I, Der G, Diez Roux A, Fitzpatrick A, Hajat A, Halaschek-Wiener J, Harris S, Hunt SC, Jagger C, Jeon HS, Kaplan R, Kimura M, Lansdorp P, Li C, Maeda T, Mangino M, Nawrot TS, Nilsson P, Nordfjall K, Paolisso G, Ren F, Riabowol K, Robertson T, Roos G, Staessen JA, Spector T, Tang N, Unryn B, van der Harst P, Woo J, Xing C, Yadegarfar ME, Park JY, Young N, Kuh D, von Zglinicki T, Ben-Shlomo Y; Halcyon study team
    • Journal Title

      Exp Gerontol

      Volume: 25 Pages: 15-27

    • Related Report
      2013 Final Research Report
  • [Journal Article] Vascular endothelial cell surviving through under prolonged elevated temperature shows persistent or transient up-regulation of telomerase and stress-associated proteins2014

    • Author(s)
      Toyoki Maeda, Jing-Zhi Guan, Masamichi Koyanagi, Naoki Makino
    • Journal Title

      Appl Cell Biol

      Volume: 2

    • Related Report
      2013 Final Research Report
  • [Journal Article] X-irradiation alters the telomerase activity and the telomere length distribution of cultured human vascular endothelial cells2014

    • Author(s)
      Toyoki Maeda, Jing-Zhi Guan, Masamichi Koyanagi, Naoki Makino
    • Journal Title

      Appl Cell Biol

      Volume: 3

    • Related Report
      2013 Final Research Report
  • [Journal Article] 別府市高齢者に置ける温泉利用の実態と既往 歴との関連の調査について2014

    • Author(s)
      前田豊樹
    • Journal Title

      日本温泉気候物理医学会雑誌

      Volume: 77 Pages: 26-28

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Vascular endothelial cell surviving through under prolonged elevated temperature shows persistent or transient up-regulation of telomerase and stress-associated proteins.2014

    • Author(s)
      Toyoki Maeda1, Jing-Zhi Guan, Masamichi Koyanagi, Naoki Makino.
    • Journal Title

      Appl Cell Biol

      Volume: 3 Pages: 0-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] X-irradiation alters the telomerase activity and the telomere length distribution of cultured human vascular endothelial cells.2014

    • Author(s)
      Toyoki Maeda1, Jing-Zhi Guan, Masamichi Koyanagi, Naoki Makino.
    • Journal Title

      Appl Cell Biol

      Volume: 3 Pages: 0-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Telomerase activity and telomere length distribution in vascular endothelial cell in a short-term culture under the presence of hydrogen peroxide2013

    • Author(s)
      Maeda T, Jing-Zhi Guan, Koyanagi M, Makino N
    • Journal Title

      Geriatrics and Gerontology International

      Volume: 13 Pages: 774-782

    • Related Report
      2013 Final Research Report
  • [Journal Article] Analysis of telomere length and subtelomeric methylation of circulating leucocytes in female patients with Alzheimer's disease2013

    • Author(s)
      J-Z. Guan, T. Maeda N. Makino
    • Journal Title

      Aging Clin Exp Res

      Volume: 25 Pages: 17-23

    • Related Report
      2013 Final Research Report
  • [Journal Article] Hyperthermia by bathing in a hot spring improves the cardiovascular functions and reduces the production of inflammatory cytokines in patients with chronic heart failure2013

    • Author(s)
      Jun-ichi Oyama, Yoshihiro Kudo, Toyoki Maeda, Koichi Node, Naoki Makino
    • Journal Title

      Heart and Vessels

      Volume: 13 Pages: 774-782

    • Related Report
      2013 Final Research Report
  • [Journal Article] Alterations in the telomere length distribution and the subtelomeric methylation status in human vascular endothelial cells under elevated temperature in culture condition2013

    • Author(s)
      Maeda T, G. Jing-Zhi, Koyanagi M Makino N
    • Journal Title

      Aging Clin Exp Res

      Volume: 25 Pages: 231-238

    • Related Report
      2013 Final Research Report
  • [Journal Article] 別府市高齢者に置ける温泉利用の実態と既往歴との関連の調査について2013

    • Author(s)
      前田豊樹
    • Journal Title

      日本温泉気候物理医学会雑誌

      Volume: 77 Pages: 26-28

    • Related Report
      2013 Final Research Report
  • [Journal Article] Analysis of telomere length and subtelomeric methylation of circulating leukocytes in women with Alzheimer's disease.2013

    • Author(s)
      Guan JZ, Guan WP, Maeda T, Makino N.
    • Journal Title

      Aging Clin Exp Res.

      Volume: 25 Issue: 1 Pages: 17-23

    • DOI

      10.1007/s40520-013-0006-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyperthermia by bathing in a hot spring improves cardiovascular functions and reduces the production of inflammatory cytokines in patients with chronic heart failure.2013

    • Author(s)
      Oyama J, Kudo Y, Maeda T, Node K, Makino N.
    • Journal Title

      Heart Vessels.

      Volume: 28 Issue: 2 Pages: 173-178

    • DOI

      10.1007/s00380-011-0220-7

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Telomerase activity and telomere length distribution in vascular endothelial cells in a short-term culture under the presence of hydrogen peroxide.2013

    • Author(s)
      Maeda T, Guan JZ, Koyanagi M, Makino N.
    • Journal Title

      Geriatr Gerontol Int.

      Volume: 13 Issue: 3 Pages: 774-782

    • DOI

      10.1111/j.1447-0594.2012.00936.x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alterations in the telomere length distribution and the subtelomeric methylation status in human vascular endothelial cells under elevated temperature in culture condition.2013

    • Author(s)
      Maeda T, Guan JZ, Koyanagi M, Makino N.
    • Journal Title

      Aging Clin Exp Res.

      Volume: 25 Issue: 3 Pages: 231-238

    • DOI

      10.1007/s40520-013-0045-6

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gender and telomere length: systematic review and meta-analysis.2013

    • Author(s)
      Gardner M, Bann D, Wiley L, Cooper R, Hardy R, Nitsch D, et al.
    • Journal Title

      Exp Gerontol.

      Volume: 51 Pages: 15-27

    • DOI

      10.1016/j.exger.2013.12.004

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different levels of hypoxia regulate telomere length and telomerase activity2012

    • Author(s)
      Jing-Zhi Guan, Wei-Ping Guan, Toyoki Maeda, Naoki Makino
    • Journal Title

      Aging Clin Exp Res

      Volume: 24 Pages: 213-217

    • Related Report
      2013 Final Research Report
  • [Journal Article] Aging-associated alteration of telomere length and subtelomeric status in female patients with Parkinson's disease2012

    • Author(s)
      Toyoki Maeda, Jing Zhi Guan, Masamichi Koyanagi, Yoshihiro Higuchi, Naoki Makino
    • Journal Title

      Neurogenetics

      Volume: 26 Pages: 245-251

    • Related Report
      2013 Final Research Report
  • [Journal Article] Alteration of telomere length and subtelomeric methylation in human endothelial cell under different levels of hypoxia2012

    • Author(s)
      Jing-Zhi Guan, Wei-Ping Guan, Toyoki Maeda, Naoki Makino
    • Journal Title

      Archives of Medical Research

      Volume: 43 Pages: 15-20

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Continuous Positive Airway Pressure Therapy Improves Vascular Dysfunction and Decreases Oxidative Stress in Patients With the Metabolic Syndrome and Obstructive Sleep Apnea Syndrome2012

    • Author(s)
      Oyama JI, Yamamoto H, Maeda T, Ito A, Node K, Makino N
    • Journal Title

      Clin Cardiol

      Volume: 35 Pages: 15-20

    • Related Report
      2013 Final Research Report
  • [Journal Article] Repetitive hyperthermia attenuates progression of left ventricular hypertrophy and increases telomerase activity in hypertensive rats2012

    • Author(s)
      Oyama JI, Maeda T, Sasaki M, Higuchi Y, Node K, Makino N
    • Journal Title

      Am J Physiol-Heart Circ Physiol

      Volume: 302

    • Related Report
      2013 Final Research Report
  • [Journal Article] Upregulation of Anticoagulant Proteins, Protein S and Tissue Factor Pathway Inhibitor, in the Mouse Myocardium with Cardio-Specific TNF- x03B1; Overexpression2012

    • Author(s)
      Yosihiro Higuchi, Masamichi Koyanagi, Toru Kubota, Toyoki Maeda, Arthur Feldman, Naoki Makino
    • Journal Title

      Am J Physiol-Heart Circ Physiol

      Volume: 302

    • Related Report
      2013 Final Research Report
  • [Journal Article] Increased Risk for CRC in Diabetic Patients with the Nonrisk Allele of SNPs at 8q242012

    • Author(s)
      Ishimaru S, Mimori K, Yamamoto K, Inoue H, Imoto S, Kawano S, Yamaguchi R, Sato T, Toh H, Iinuma H, Maeda T, Ishii H, Suzuki S, Tokudome S, Watanabe M, Tanaka JI, Kudo SE, Sugihara KI, Hase K, Mochizuki H, Kusunoki M, Yamada K, Shimada Y, Moriya Y, Barnard GF, Miyano S, Mori M
    • Journal Title

      Ann Surg Oncol

      Volume: 19 Pages: 2853-2858

    • Related Report
      2013 Final Research Report
  • [Journal Article] The gender-related alterations in the telomere length and subtelomeric methylation status in patients with Parkinson's disease2012

    • Author(s)
      Maeda T, Jing-Zhi Guan, Koyanagi M, Makino N
    • Journal Title

      Ageing Res

      Volume: 4 Pages: 61-66

    • Related Report
      2013 Final Research Report
  • [Journal Article] Radiation- associated telomere length changes of peripheral leukocytes of inpatients with cancer International2012

    • Author(s)
      Toyoki Maeda, Katsumasa Nakamura, Kazushige Atsumi, Masakazu Hirakawa, Yasuko Ueda, Naoki Makino
    • Journal Title

      Journal of Radiation Biology

      Volume: 89 Pages: 106-109

    • Related Report
      2013 Final Research Report
  • [Journal Article] Effect of vitamin E administration on the elevated oxygen stress and the telomeric and subtelomeric status in Alzheimer's disease.2012

    • Author(s)
      Guan JZ, Guan WP, Maeda T, Makino N.
    • Journal Title

      Gerontology

      Volume: 58 Issue: 1 Pages: 62-69

    • DOI

      10.1159/000327821

    • Related Report
      2012 Research-status Report 2011 Research-status Report
  • [Journal Article] Continuous positive airway pressure therapy improves vascular dysfunction and decreases oxidative stress in patients with the metabolic syndrome and obstructive sleep apnea syndrome.2012

    • Author(s)
      Oyama J, Yamamoto H, Maeda T, Ito A, Node K, Makino N.
    • Journal Title

      Clinical Cardiolology

      Volume: 35 Issue: 4 Pages: 231-236

    • DOI

      10.1002/clc.21010

    • Related Report
      2012 Research-status Report
  • [Journal Article] Aging-associated alteration of telomere length and subtelomeric status in female patients with Parkinson's disease.2012

    • Author(s)
      Maeda T, Guan JZ, Koyanagi M, Higuchi Y, Makino N.
    • Journal Title

      Journal of Neurogenetics

      Volume: 26 Issue: 2 Pages: 245-251

    • DOI

      10.3109/01677063.2011.651665

    • Related Report
      2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Alteration of telomere length and subtelomeric methylation in human endothelial cell under different levels of hypoxia.2012

    • Author(s)
      Guan JZ, Guan WP, Maeda T, Makino N.
    • Journal Title

      Archives of Medical Research

      Volume: 43 Issue: 1 Pages: 15-20

    • DOI

      10.1016/j.arcmed.2012.02.001

    • Related Report
      2012 Research-status Report
  • [Journal Article] Repetitive hyperthermia attenuates progression of left ventricular hypertrophy and increases telomerase activity in hypertensive rats.2012

    • Author(s)
      Oyama J, Maeda T, Sasaki M, Higuchi Y, Node K, Makino N.
    • Journal Title

      American Journal of Physiology Heart Circulation Physiology

      Volume: 302 Issue: 10 Pages: 2092-2101

    • DOI

      10.1152/ajpheart.00225.2011

    • Related Report
      2012 Research-status Report
  • [Journal Article] The Subtelomere of Short Telomeres is Hypermethylated in Alzheimer's Disease.2012

    • Author(s)
      Guan JZ, Guan WP, Maeda T, Makino N.
    • Journal Title

      Aging and Disease

      Volume: 3 Pages: 164-170

    • Related Report
      2012 Research-status Report
  • [Journal Article] Different levels of hypoxia regulate telomere length and telomerase activity.2012

    • Author(s)
      Guan JZ, Guan WP, Maeda T, Makino N.
    • Journal Title

      Aging and Clinical Experimental Research

      Volume: 24 Pages: 213-217

    • Related Report
      2012 Research-status Report
  • [Journal Article] Different levels of hypoxia regulate telomere length and telomerase activity.2012

    • Author(s)
      Guan JZ, Guan WP, Maeda T, Makino.
    • Journal Title

      Aging Clin Exp Res

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 種々の臨床検査値と末梢血白血球テロメア関連パラメータとの連関の解析2011

    • Author(s)
      前田豊樹,小柳雅孔,尾山純一,樋口義洋,牧野直樹
    • Journal Title

      適応医学

      Volume: 14 Pages: 50-59

    • Related Report
      2013 Final Research Report
  • [Journal Article] Antioxidant Therapy Attenuates Myocardial Telomerase Activity Reduction in Superoxide Dismutase-Deficient Mice2011

    • Author(s)
      Makino N, Maeda T, Oyama JI, Sasaki M, Higuchi Y, Mimori K, Shimizu T
    • Journal Title

      J Mol Cell Cardiol

      Volume: 50 Pages: 670-677

    • NAID

      120004951631

    • Related Report
      2013 Final Research Report
  • [Journal Article] Telomerase inhibition promotes an initial step of cell differentiation of primate embryonic stem cell2011

    • Author(s)
      Maeda T, Kurita R, Yokoo T, Tani K, Makino N
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 407 Pages: 491-494

    • Related Report
      2013 Final Research Report
  • [Journal Article] The effect of vitamin E administration on the elevated oxygen stress and the telomeric and subtelomeric status in Alzheimer's disease2011

    • Author(s)
      Jing-Zhi Guan, Wei-Ping Guan, Toyoki Maeda, Naoki Makino
    • Journal Title

      Gerontology

      Volume: 58 Pages: 62-69

    • Related Report
      2013 Final Research Report
  • [Journal Article] The subtelomere of short telomeres is hypermethylated in Alzheimer's disease2011

    • Author(s)
      Jing-Zhi Guan, Toyoki Maeda, Naoki Makino
    • Journal Title

      Aging and Disease

      Volume: 3 Pages: 164-170

    • Related Report
      2013 Final Research Report
  • [Journal Article] 種々の臨床検査値と末梢血白血球テロメア関連パラメータとの連関の解析.2011

    • Author(s)
      前田豊樹,小柳雅孔,尾山純一,樋口義洋,牧野直樹
    • Journal Title

      適応医学

      Volume: 14 Pages: 50-59

    • Related Report
      2011 Research-status Report
  • [Presentation] DNAと老化と病気2013

    • Author(s)
      前田豊樹
    • Organizer
      平成25年度別府女医会
    • Place of Presentation
      別府
    • Year and Date
      2013-05-17
    • Related Report
      2013 Final Research Report
  • [Presentation] 心エコー検査による心機能評価と白血球テロメア長の相関について2013

    • Author(s)
      前田豊樹、牧野直樹
    • Organizer
      第23回日本老年医学会九州地方会
    • Place of Presentation
      福岡
    • Year and Date
      2013-03-09
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 別府市高齢者における温泉利用の実態と既往歴との関連の調査について2013

    • Author(s)
      前田豊樹
    • Organizer
      第78回日本温泉気候物理医学会(シンポジウム)
    • Place of Presentation
      別府
    • Related Report
      2013 Final Research Report
  • [Presentation] Telomere length distribution of circulating leukocytes in patients with Alzheimer's disease2013

    • Author(s)
      Toyoki Maeda
    • Organizer
      XXII World Congress of Neurology
    • Place of Presentation
      Vienna
    • Related Report
      2013 Final Research Report
  • [Presentation] 別府市高齢者における温泉利用の実態と既往歴との関連の調査について2013

    • Author(s)
      前田豊樹
    • Organizer
      第78回日本温泉気候物理医学会
    • Place of Presentation
      別府
    • Related Report
      2013 Annual Research Report
  • [Presentation] Telomere length distribution of circulating leukocytes in patients with Alzheimer’s disease.2013

    • Author(s)
      前田豊樹
    • Organizer
      XXII World Congress of Neurology
    • Place of Presentation
      Vienna
    • Related Report
      2013 Annual Research Report
  • [Presentation] 温熱効果のテロメアDNA老化性変化への影響の解析2012

    • Author(s)
      前田豊樹
    • Organizer
      平成23年度日本健康開発財団研究助成発表会
    • Place of Presentation
      東京
    • Year and Date
      2012-03-12
    • Related Report
      2013 Final Research Report
  • [Presentation] パーキンソン病女性患者における末梢白血球テロメア長の変化について2012

    • Author(s)
      前田豊樹、牧野直樹
    • Organizer
      第22回日本老年医学会九州地方会
    • Place of Presentation
      佐賀
    • Year and Date
      2012-03-03
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 鉱泥浴治療効果の客観的指標の探索2012

    • Author(s)
      前田豊樹、工藤義弘、西山保弘、牧野直樹
    • Organizer
      第77回日本温泉気候物理学会
    • Place of Presentation
      仙北
    • Related Report
      2013 Final Research Report
  • [Presentation] アルツハイマー病女性患者における末梢白血球テロメア長の変化について2012

    • Author(s)
      前田豊樹、牧野直樹
    • Organizer
      第54回日本老年医学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 鉱泥浴治療効果の客観的指標の探索2012

    • Author(s)
      前田豊樹、工藤義弘、西山保弘、牧野直樹
    • Organizer
      第77回に本温泉気候物理学会
    • Place of Presentation
      仙北
    • Related Report
      2012 Research-status Report
  • [Presentation] 外来男性生活習慣病患者における各種臨床検査値と末梢血白血球テロメア長関連パラメータについて2011

    • Author(s)
      前田豊樹、牧野直樹
    • Organizer
      第53回日本老年医学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2011-06-16
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Book] ヒトの分子遺伝学(翻訳)第4版(第21章)小南凌監修2011

    • Author(s)
      村松正實
    • Publisher
      メディカル・サイエンス・インターナショナル
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.beppu.hosp.kyushu-u.ac.jp/naika/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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