A genome-wide screenig of autoantibody for eating disorders using the protein active array

• Project/Area Number: 23590908
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: ANDO Tetsuya 独立行政法人国立精神・神経医療研究センター, 精神保健研究所・心身医学研究部, 室長 (50311428)
• Co-Investigator(Kenkyū-buntansha): GOSHIMA Naoki 独立行政法人産業技術総合研究所創薬分子プロファイリング研究センター, 細胞システム制御解析チーム (70215482)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 摂食障害 / 神経性無食欲症 / 神経性大食症 / 肥満 / プロテインアレイ / 自己抗体 / 蛋白質 / 抗原

Research Abstract

Serum autoantibodies specific for eating disorders were screened using a protein array loaded with approximately 20,000 proteins, and 355 positive spots were detected. Of those, 180 antigens showing the strong signal were subjected to further confirmation in independent samples of eating disorder, obesity and healthy control. Forty seven antigens showed the positive signal in the anorexia nervosa sample, while negative in the control sample. The antigens with high positive rates included proteins which play a role in intracellular trafficking, regulation of G protein signaling, gastrointestinal functions and food intake, and neuroendocrine stress responses. A gene set analysis indicated that the positive antigens were included significantly more often in the gene set of Hormone_Activity.

Research Products

• [Journal Article] The Japanese Genetic Research Group For Eating Disorders. Association of the c.385C>A (p.Pro129Thr) polymorphism of the fatty acid amide hydrolase gene with anorexia nervosa in the Japanese population (2014)
  • Author(s): Ando T, Tamura N, Mera T, Morita C, Takei M, Nakamoto C, Koide M, Hotta M, Naruo T, Kawai K, Nakahara T, Yamaguchi C, Nagata T, Ookuma K, Okamoto Y, Yamanaka T, Kiriike N, Ichimaru Y, Ishikawa T, Komaki G
  • Journal Title: Molecular Genetics & Genomic Medicine Volume: 0 Issue: 4 Pages: 0-0
  • DOI: 10.1002/mgg3.69
  • Peer Reviewed
• [Journal Article] A genome-wide association study of anorexia nervosa (2014)
  • Author(s): Boraska V, Franklin CS, Floyd JA, Thornton LM, Huckins LM, Southam L, Rayner NW, Tachmazidou I, Klump KL, Treasure J, Lewis CM, Schmidt U, Tozzi F, Kiezebrink K, Hebebrand J, Gorwood P, Adan RA, Kas MJ, Favaro A, Santonastaso P, Fernández-Aranda F, Gratacos M, Rybakowski F, Dmitrzak-Weglarz M, Kaprio J, Keski-Rahkonen A, Raevuori A, Van Furth EF, Slof-Op 't Landt MC, Hudson JI, Reichborn-Kjennerud T, Knudsen GP, Monteleone P, Kaplan AS, Karwautz A, Hakonarson H, Berrettini WH, Guo Y, Li D, Schork NJ, Komaki G, Ando T, Inoko H, et al
  • Journal Title: Molecular Psychiatry Volume: 0 Issue: 10 Pages: 1-10
  • DOI: 10.1038/mp.2013.187
  • Peer Reviewed
• [Journal Article] 摂食障害研究の最近の動向 (2014)
  • Author(s): 安藤哲也
  • Journal Title: 心身医学 Volume: 54(2) Pages: 146-153
• [Journal Article] Detection of tumor-associated antigens in culture supernatants using autoantibodies in sera from patients with bladder cancer (2014)
  • Author(s): Minami S, Nagashio R, Ueda J, Matsumo K, Goshima N, Hattori M, Hachimura K, Iwamura M, and Sato Y
  • Journal Title: Biomedical Research (Tokyo) Volume: 53(1) Pages: 25-35
  • Peer Reviewed
• [Journal Article] Detection of tumor-associated antigens in culture supernatants using autoantibodies in sera from patients with bladder cancer. (2014)
  • Author(s): Minami S, Nagashio R, Ueda J, Matsumo K, Goshima N, Hattori M, Hachimura K, Iwamura M, and Sato Y
  • Journal Title: Biomedical Research (Tokyo) Volume: 53（1） Pages: 25-35
  • Peer Reviewed
• [Journal Article] 摂食障害と遺伝子 (2013)
  • Author(s): 安藤哲也
  • Journal Title: 臨床精神医学 Volume: 42(5) Pages: 609-620
• [Journal Article] Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. (2013)
  • Author(s): Yugawa T, Nishino K, Ohno S, Nakahara T, Fujita M, Goshima N, Umezawa A, Kiyono T
  • Journal Title: Molecular and Cellular Biology Volume: 33(22) Issue: 22 Pages: 4434-4447
  • DOI: 10.1128/mcb.00577-13
  • Peer Reviewed
• [Journal Article] Integrated view of the human chromosome X-centric proteome project. (2013)
  • Author(s): Yamamoto T, Nakayama K, Hirano H, Tomonaga T, Ishihama Y, Yamada T, Kondo T,Kodera Y, Sato Y, Araki N, Mamitsuka H, Goshima N.
  • Journal Title: J Proteome Res. Volume: 12 Issue: 1 Pages: 58-61
  • DOI: 10.1021/pr300844p
  • Peer Reviewed
• [Journal Article] No association of Brain-Derived Neurotrophic Factor Val66Met Polymorphism with Anorexla Nervosa in Japanese (2012)
  • Author(s): Ando T, et al., 22人中13番目
  • Journal Title: Am J Med Genet Part B Volume: 159B Issue: 1 Pages: 48-52
  • DOI: 10.1002/ajmg.b.32000
  • Peer Reviewed
• [Journal Article] CAXII is sero-diagnostic marker for lung cancer (2012)
  • Author(s): Kobayashi M, Matsumoto T, Sato Y, et al
  • Journal Title: PLoS ONE Volume: 7 (3) Issue: 3 Pages: e33952-e33952
  • DOI: 10.1371/journal.pone.0033952
  • Peer Reviewed
• [Journal Article] Cancer-testis antigen BORIS is a novel prognostic marker for patients with esophageal cancer. (2012)
  • Author(s): Okabayashi K, Fujita T, Miyazaki J, Okada T, Iwata T, Hirao N, Noji S, Tsukamoto N, Goshima N, Hasegawa H, Takeuchi H, Ueda M, Kitagawa Y, Kawakami Y.
  • Journal Title: Cancer Sci Volume: 103(9) Issue: 9 Pages: 1617-24
  • DOI: 10.1111/j.1349-7006.2012.02355.x
  • Peer Reviewed
• [Presentation] Plasma amino acid profile in anorexia nervosa and its relation to current food intake (2014)
  • Author(s): Ando T, Tamura N, Ichimaru Y, Kura N, Konishi M, Tomita Y, Chiba N, Ishikawa T and Komaki G
  • Organizer: International Conference on Eating Disorders
• [Presentation] 脂肪酸アミド加水分解酵素(FAAH)遺伝子385C/A多型と神経性食欲不振症との関連 (2013)
  • Author(s): 安藤哲也,田村奈穂,米良貴嗣,河合啓介,武井美智子,中本智恵美,鈴木(堀田)眞理,成尾鉄朗,石川俊男,小牧元
  • Organizer: 第17回日本摂食障害学会・学術集会
  • Place of Presentation: 神戸市産業振興センター,神戸
• [Presentation] 神経性食欲不振症の血漿アミノ酸プロファイルの解析 (2013)
  • Author(s): 安藤哲也,田村奈穂,倉尚樹,小西恵,富田吉敏,知場奈津子,本間洋州,濱田孝,石川俊男,小牧元
  • Organizer: 第54回日本心身医学会学術講演会
  • Place of Presentation: パシフィコ横浜,横浜
• [Presentation] 脂肪酸アミド加水分解酵素（FAAH）遺伝子385C/A 多型と神経性食欲不振症との関連 (2013)
  • Author(s): 安藤哲也、田村奈穂、米良貴嗣、河合啓介、武井美智子、中本智恵美、鈴木（堀田）眞理、成尾鉄朗、石川俊男、小牧 元
  • Organizer: 第17回日本摂食障害学会・学術集会
  • Place of Presentation: 神戸市産業振興センター、神戸市
• [Presentation] In vitro proteome analysis by using genome-wide human proteome expression resources (HUPEX) (2012)
  • Author(s): 五島直樹
  • Organizer: 第35回日本分子生物学会年会ワークショップ
  • Place of Presentation: 福岡国際会議場,福岡
  • Year and Date: 2012-12-12
• [Presentation] 神経性食欲不振症の血漿アミノ酸濃度の解析 (2012)
  • Author(s): 安藤哲也,田村奈穂,倉尚樹,東風谷祐子,小西恵,富田吉敏,知場奈津子,庭瀬亜香,濱田孝,杤久保修,市丸雄平,石川俊男,小牧元
  • Organizer: 第16回日本摂食障害学会学術集会
  • Place of Presentation: 政策医療大学院大学,港区,東京
• [Presentation] 神経性食欲不振症の血漿アミノ酸濃度の解析 (2012)
  • Author(s): 安藤哲也、田村奈穂、倉 尚樹、東風谷祐子、小西 恵、富田吉敏、知場奈津子、庭瀬 亜香、濱田 孝、杤久保 修、市丸雄平、石川俊男、小牧 元
  • Organizer: 第16回日本摂食障害学会学術集会
  • Place of Presentation: 政策医療大学院大学、港区、東京
• [Presentation] In vitro proteome analysis by using genome-wide human proteome expression resources (HUPEX) (2012)
  • Author(s): 五島直樹
  • Organizer: 第35回日本分子生物学会年会 ワークショップ
  • Place of Presentation: 福岡国際会議場
• [Presentation] 摂食障害遺伝子解析研究協力者会議脳由来神経栄養因子遺伝子の多型と神経性食欲不振症との関連の検討 (2011)
  • Author(s): 安藤哲也,石川俊男,鈴木(堀田)眞理,成尾鉄朗,岡部憲二郎,中原敏博,瀧井正人,河合啓介,米良貴嗣,中本智恵美,武井美智子,山口力,永田利彦,岡本百合,大隈和喜,小出将則,山中隆夫,田村奈穂,切池信夫,小牧元
  • Organizer: 第52回日本心身医学会総会ならびに学術講演会
  • Place of Presentation: パシフィコ横浜,横浜
• [Presentation] 神経性食欲不振症における血漿アミノ酸濃度プロフィールの検討 (2011)
  • Author(s): 安藤哲也,田村奈穂,山本浩史,倉尚樹,小西恵,富田吉敏,田中孝幸,知場奈津子,庭瀬亜香,濱田孝,杤久保修,石川俊男,小牧元
  • Organizer: 第16回日本心療内科学会総会・学術大会
  • Place of Presentation: 東京国際交流館・プラザ平成,東京
• [Presentation] 神経性食欲不振症における血漿アミノ酸濃度プロフィールの検討 (2011)
  • Author(s): 安藤哲也、田村奈穂、他
  • Organizer: 第16回日本心療内科学会総会・学術大会
  • Place of Presentation: 東京国際交流館・プラザ平成，東京
• [Presentation] 脳由来神経栄養因子遺伝子の多型と神経性食欲不振症との関連の検討 (2011)
  • Author(s): 安藤哲也、石川俊男、他
  • Organizer: 第52回日本心身医学会総会ならびに学術講演会
  • Place of Presentation: パシフィコ横浜，横浜
• [Book] Ghrelin Gene Variants and Eating Disorders. In : Anorexia, Litwack, G Ed. Vitamins and Hormones, Vol 92 (2013)
  • Author(s): Ando T
  • Publisher: Academic Press
• [Book] Ghrelin Gene Variants and Eating Disorders. In: Anorexia, Litwack, G Ed. Vitamins and Hormones, Vol 92 (2013)
  • Author(s): Ando T.
  • Total Pages: 17
  • Publisher: Academic Press
• [Book] 新機能抗体開発ハンドブック第1章5節アレイを用いた自己抗体解析 (2012)
  • Author(s): 五島直樹
  • Publisher: ㈱エヌ・ティー・エス
• [Book] 新機能抗体開発ハンドブック 第1章５節 アレイを用いた自己抗体解析 (2012)
  • Author(s): 五島直樹
  • Total Pages: 5
  • Publisher: ㈱エヌ・ティー・エス