The role of AID expression in the development of EBV associated gastric cancer

• Project/Area Number: 23590918
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Yamaguchi University
• Principal Investigator: NISHIKAWA Jun 山口大学, 医学部附属病院, 准教授 (00379950)
• Co-Investigator(Kenkyū-buntansha): OGA Atsunori 山口大学, 大学院医学系研究科, 講師 (90243633)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Epstein-Barr virus / 胃癌 / AID / DNAメチル化 / 残胃癌

Research Abstract

We analyzed AID expression in EBV-associated Gastric cancers by immunohistochemistry, however,specific reaction by the antibody could not be detected in the paraffin-embedded tissue section. High-density DNA oligonucleotide arrays was performed in mononuclear cells of the patients with EBV-associated Gastric cancers. Copy number variation which specifically occured in the patients will be identified from big data. The association of DNA methylation in the development of EBV-associated Gastric cancers was also examined. We found several tumor suppressor genes whose promoter regions were specificcly methylated in EBV-associated Gastric cancers.Inactivation of these genes may suppress their function as tumor suppressor genes or tumor-associated antigen to develop and maintain EBVaGCs.

Research Products

• [Journal Article] Colonization of an acid resistant Kingella denitrificans in the stomach may contribute to gastric dysbiosis by Helicobacter pylori (2014)
  • Author(s): Okamoto T, Hayashi Y, Mizuno H, Yanai H, Nishikawa J, Nakazawa T, Iizasa H, Jinushi M, Sakaida I, Yoshiyama H
  • Journal Title: J Infect Chemother Volume: 20(3) Pages: 169-74
  • Peer Reviewed
• [Journal Article] Identification of genes specifically methylated in Epstein-Barr virus-associated gastric carcinomas (2013)
  • Author(s): Okada T, Nakamura M, Nishikawa J, Sakai K, Zhang Y, Saito M, Morishige A, Oga A, Sasaki K, Suehiro Y, Hinoda Y, Sakaida I
  • Journal Title: Cancer Sci Volume: 104(10) Pages: 1309-14
  • Peer Reviewed
• [Journal Article] Role of DNA methylation in the development of Epstein-Barr virus-associated gastric carcinoma (2013)
  • Author(s): Saito M, Nishikawa J, Okada T, Morishige A, Sakai K, Nakamura M, Kiyotoki S, Hamabe K, Okamoto T, Oga A, Sasaki K, Suehiro Y, Hinoda Y, Sakaida I
  • Journal Title: J Med Virol Volume: 85(1) Pages: 121-7
  • Peer Reviewed
• [Journal Article] Identification of genes specifically methylated in Epstein-Barr virus-associated gastric carcinomas. (2013)
  • Author(s): Okada T, Nakamura M, Nishikawa J, Sakai K, Zhang Y, Saito M, Morishige A, Oga A, Sasaki K, Suehiro Y, Hinoda Y, Sakaida I.
  • Journal Title: Cancer Sci Volume: 104 Issue: 10 Pages: 1309-1314
  • DOI: 10.1111/cas.12228
  • Peer Reviewed
• [Journal Article] Role of DNA methylation in the development of Epstein-Barr virus-associated gastric carcinoma. (2013)
  • Author(s): Saito M, Nishikawa J, Okada T, Morishige A, Sakai K, Nakamura M, Kiyotoki S, Hamabe K, Okamoto T, Oga A, Sasaki K, Suehiro Y, Hinoda Y, Sakaida I.
  • Journal Title: J Med Virol. Volume: 85 Issue: 1 Pages: 121-127
  • DOI: 10.1002/jmv.23405
  • Peer Reviewed
• [Journal Article] Epstein-Barr Virus (EBV)-associated Gastric Carcinoma Viruses (2012)
  • Author(s): IizasaH , Nanbo A, NishikawaJ, Jinushi M and Yoshiyama H
  • Volume: 4(12)
  • Pages: 3420-3439
  • Peer Reviewed
• [Journal Article] EBV-associated gastric carcinoma (2012)
  • Author(s): Iizasa H, Nanbo A, Nishikawa J, Jinushi M, Yoshiyama H
  • Journal Title: Viruses Volume: 4 Pages: 3420-3439
  • Peer Reviewed
• [Presentation] IDENTIFICATION OF GENES SPECIFICALLY METHYLATED IN EPSTEIN-BARR VIRUS ASSOCIATED GASTRIC CARCINOMAS (2013)
  • Author(s): Jun Nishikawa , Munetaka Nakamura , Kouhei Sakai , Atsushi Goto ,Junichi Nishimura , Takeshi Okamoto, Yutaka Suehiro , Isao Sakaida
  • Organizer: UEGW2013(欧州消化器病学会週間)
  • Place of Presentation: Berlin
  • Year and Date: 2013-10-15
• [Presentation] Epstein-Barr virus関連胃癌の病態解明から治療への展開 (2013)
  • Author(s): 西川潤、中村宗剛、坂井田功
  • Organizer: 第85回日本消化器病内視鏡学会総会
  • Place of Presentation: 京都(国立京都国際会館)
• [Presentation] Epstein-Barr virus 関連胃癌の病態解明から治療への展開 (2013)
  • Author(s): 西川 潤、中村宗剛、坂井田功
  • Organizer: 第85回 日本消化器病内視鏡学会総会
  • Place of Presentation: 京都（国立京都国際会館）
• [Presentation] EBV陽性胃癌に対する脱メチル化剤の抗腫瘍効果と上皮間葉移行への影響 (2012)
  • Author(s): 中村宗剛,西川潤,五嶋敦史,西村純一,岡本健志.酒井幸平,末広寛,坂井田功
  • Organizer: 第16回中国消化管スタディーグループ研究会
  • Place of Presentation: 宇部(ANAクラウンプラザホテル宇部)
  • Year and Date: 2012-11-30
• [Presentation] Epstein-Barr virus関連胃癌の発生におけるDNAメチル化の意義 (2012)
  • Author(s): 西川潤、齋藤真理、岡田季之、末広寛、坂井田功
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌(ロイトン札幌)
• [Presentation] Epstein-Barr virus関連胃癌の発生におけるDNAメチル化の意義 (2012)
  • Author(s): 西川 潤、齋藤真理、岡田季之、末広 寛、坂井田功
  • Organizer: 第71回日本癌学会
  • Place of Presentation: ロイトン札幌（札幌）
• [Presentation] Epstein-Barr virus関連胃癌特異的なDNAメチル化異常とその誘導機序について (2011)
  • Author(s): 西川潤、斎藤真理、浜辺功一、五嶋敦史、岡本健志、坂井田功
  • Organizer: 第53回日本消化器病学会大会
  • Place of Presentation: 福岡(福岡国際センター)
• [Presentation] Epstein-Barr virus関連胃癌の特性に合わせた脱メチル化剤による治療 (2011)
  • Author(s): 西川潤、齊藤真理、坂井田功
  • Organizer: 第97回日本消化器病学会総会
  • Place of Presentation: 東京(京王プラザホテル)
• [Presentation] Epstein-Barr virus関連胃癌におけるDNAメチル化について (2011)
  • Author(s): 齋藤 真理,西川 潤,清時 秀,浜辺 功一,岡本 健志,坂井田 功
  • Organizer: 第97回日本消化器病学会総会
  • Place of Presentation: 京王プラザホテル（東京）