The expression of stem cell marker in intestinal metaplastic mucosa

• Project/Area Number: 23590926
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Jichi Medical University
• Principal Investigator: KENSUKE Yokoyama 自治医科大学, 医学部, 臨床助教 (40598926)
• Co-Investigator(Kenkyū-buntansha): MUTOH Hiroyuki 自治医科大学, 医学部, 教授 (50322392)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Lgr5 / Cdx2 / 幹細胞 / DCAMKL1 / Bmi1 / 幹細胞マーカー

Outline of Final Research Achievements

Transcription factor Cdx2 is expressed in the intestinal metaplastic mucosa. Cdx2 induces intestinal metaplasia in the stomach in the Cdx2 transgenic mice. Lgr5, CD133 and DCAMKL1 are expressed in the intestinal metaplasia of human and Cdx2 transgenic mouse stomach.CD133 and Lgr5 are reported to be related to the cancer stem cell. The relationships between the gastric carcioma and cancer stem cell in the Cdx2-transgenic mice were examined. The mechanisms of the proliferation and differentiation of cancer stem cell were examined. The clarification of the stem cell marker in the intestinal metaplastic mucosa may be related to the regenerative medicine and cancer therapy.

Research Products

• [Journal Article] Comparative analysis of gastrointestinal microbiota between normal and caudal-related homeobox 2 (cdx2) transgenic mice. (2015)
  • Author(s): Sakamoto H, Asahara T, Chonan O, Yuki N, Mutoh H, Hayashi S, Yamamoto H, Sugano K.
  • Journal Title: Intest Res. Volume: 13 Issue: 1 Pages: 39-34
  • DOI: 10.5217/ir.2015.13.1.39
  • Peer Reviewed / Open Access
• [Journal Article] Cyclooxygenase 2 in gastric carcinoma is expressed in doublecortin- and CaM kinase-like-1-positive tuft cells. (2014)
  • Author(s): Mutoh H, Sashikawa M, Sakamoto H, Tateno T.
  • Journal Title: Gut Liver Volume: 8 Issue: 5 Pages: 508-518
  • DOI: 10.5009/gnl13237
  • Peer Reviewed / Open Access
• [Journal Article] Cyclooxygenase 2 in gastric carcinoma is expressed in doublecortin and CaM kinase-like-1-positive tuft cells. (2014)
  • Author(s): Mutoh H, Sashikawa M, Sakamoto H, Tateno T
  • Journal Title: Gut Liver Volume: in press
  • Peer Reviewed
• [Journal Article] SOX9 Is Highly Expressed in Nonampullary Duodenal Adenoma and Adenocarcinoma in Humans. (2013)
  • Author(s): Sakamoto H, Mutoh H, Miura Y, Sashikawa M, Yamamoto H, Sugano K
  • Journal Title: Gut Liver Volume: 7 Pages: 513-518
  • Peer Reviewed
• [Journal Article] CDX1 directs gastric intestinal metaplasia via induction of stemness-associated reprogramming factors SALL4 and KLF5 (2012)
  • Author(s): Fujii, Y.
  • Journal Title: Proc. Natl. Acad. Sci.USA Volume: 109 Issue: 50 Pages: 20584-20589
  • DOI: 10.1073/pnas.1208651109
  • Peer Reviewed