| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
[Aim] COX2 plays a critical role in peptic ulcer development. We evaluated COX2 methylation levels in the gastric mucosa. [Methods] DNA was extracted from endoscopic biopsy materials. The COX2 methylation levels were measured quantitatively by pyrosequencing. COX2 mRNA expression was measured using real-time PCR. [Results] COX2 methylation levels were significantly higher in H.pylori (HP)-positive cases than in HP-negative cases. COX2 methylation levels in patients in whom HP was successfully eradicated were significantly lower than that in HP-positive cases. COX2 mRNA expression was not observed in Kato III cells, despite the addition of the protein kinase C stimulator (PDBu). COX2 expression was observed after the addition of the demethylating agent and was enhanced by PDBu.
[Conclusion] HP infection caused a significant increase in the COX2 methylation levels in the gastric mucosa. In addition to transcriptional regulation, COX2 expression is regulated through epigenetic mechanisms.
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