| Project/Area Number |
23590936
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
BAN Hiromitsu 滋賀医科大学, 医学部, 特任助教 (30598363)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 炎症性腸疾患 / 潰瘍性大腸炎 / 薬剤感受性遺伝子 / 遺伝子一塩基多型 / MRP4 / クローン病 / ITPase / TPMT |
|---|
| Research Abstract |
Sample DNA of ulcerative colitis, the Crohn disease patient who collected it by 2014 and the physically unimpaired person volunteer examined 279 personality in total, SNP of 3 genes in the both sides of the TaqMan probe method and the conventional Direct sequence method. As for the MRP4 G2269A SNP, 68 people, A/A were seven people G/A, and the allyl frequency was 14.7%. As for the TPMT A719G SNP, five people, G/G were 0 people A/G, and the allyl frequency was 0.9%. As for the ITPA C94A SNP, 61 people, A/A were six people C/A, and the allyl frequency was 13.1%. There was no great difference and a result of HapMap, and there was a difference by the TaqMan probe method and the Direct sequence method neither. It was revealed that the TaqMan probe method using TaqMan SNP Genotyping Assays was more useful for quick SNP analysis than the above-mentioned result.
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