| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
The role of regulatory B cells (Bregs) producing IL-10 in the pathogenesis of Crohn's diseases (CD) remains unknown. We investigated IL-10 production in B cells from CD patients, as well as immunoregulatory functions of Bregs in experimental colitis mouse models. CpG DNA-induced IL-10 production was decreased in B cells from CD patients, as compared to those from healthy controls, while Bregs were found to be enriched in a population of CD19(hi)and CD1d(hi) B cells isolated from both human and mouse samples. The severity of intestinal inflammation was increased in the Breg-depleted mice, with similar results also found in adoptive transfer colitis model mice even after regulatory T cells (Tregs) depletion. Our findings show that Bregs reduced experimental colitis regardless of the presence or absence of Tregs. These results suggest that a deficiency or decrease of Bregs function exacerbates intestinal inflammation, which may be associated with the pathogenesis of CD.
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