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Analysis of the role of HBx in the HBV-related hepatocarninogenesis

Research Project

Project/Area Number 23590964
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionThe University of Tokyo

Principal Investigator

YOTSUYANAGI Hiroshi  東京大学, 医学部附属病院, 准教授 (30251234)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsB型肝炎ウイルス / 組み込み / X遺伝子 / B型肝炎ウイルス / X遺伝子 / 遺伝子組み込み
Research Abstract

First we analyzed the integration of HBx gene into the chromosome of PLC/PRF/5 cells using fluorescence in situ hybridization, which disclosed the integration of HBx into plural chromosomes. For further analysis, we determined the integrated HBV sequences with the adjacent host genes using next generation sequencer, which disclosed that HBV was integrated into 11 chromosomes. On chromosome 5, HBx gene was integrated into the promoter region of hTERT. The methylation of CpG island in the HBV and adjacent host DNAs were low, which suggested that methylation may play some role in the HBV-associated hepatocarcinogenesis.
Next we analyzed HBV-HCC samples obtained on hepatectomy. HBV was integrated into multiple host DNA, including SLC6A13 and FN1.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (8 results)

All 2014 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] A new detection method for integration of hepatitis B virus DNA in PLC/PRF/5 Cells2012

    • Author(s)
      Hiraishi T, Watanabe Y, Oikawa R, Hara M, Kiyokawa H, Yoshida Y, Yamada N, Okuse C, Suzuki M, Yotsuyanagi H, Itoh F
    • Journal Title

      St. Marianna Universirty Journal

      Volume: 3 Pages: 63-72

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Pathogenesis of lipid metabolism disorder in hepatitis C: polyunsaturated fatty acids counteract lipid alterations induced by the core protein.2011

    • Author(s)
      Miyoshi H, Moriya K, Tsutsumi T, Shinzawa S, Fujie H, Shintani Y, Fujinaga H, Goto K, Todoroki T, Suzuki T, Miyamura T, Matsuura Y, Yotsuyanagi H, Koike K.
    • Journal Title

      J. Hepatol

      Volume: 54 Issue: 3 Pages: 432-438

    • DOI

      10.1016/j.jhep.2010.07.039

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article]

    • Author(s)
      Watanabe Y, Yamamoto H, Oikawa R, Toyoda M, Arii S, Yotsuyanagi H, Itoh F, Koike K
    • Related Report
      2013 Final Research Report
  • [Presentation] B型肝細胞癌における組み込みの意味2014

    • Author(s)
      四柳宏,渡邊嘉行,山本博幸,及川律子,山田典栄,森屋恭爾,伊東文生,小池和彦
    • Organizer
      日本肝臓学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] B型肝細胞癌における組み込みの意味2014

    • Author(s)
      四柳宏 渡邉嘉行 山本博幸 平石哲也 及川律子 山田典栄 森屋恭爾 伊東文生 小池和彦
    • Organizer
      日本肝臓学会総会
    • Place of Presentation
      東京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] PLC/PRF/5細胞株におけるB型肝炎ウイルスDNAの組み込み形態2012

    • Author(s)
      平石哲也,渡邊嘉行,及川律子,重福隆太,大石嘉恭,山田典栄,高橋秀明,奥瀬千晃,四柳宏,伊東文生
    • Organizer
      日本消化器病学会総会
    • Place of Presentation
      東京都
    • Related Report
      2013 Final Research Report
  • [Presentation] PLC/PRF/5細胞株におけるB型肝炎ウイルスDNAの組み込み形態2012

    • Author(s)
      平石哲也, 渡邊嘉行, 及川律子, 重福隆太, 大石嘉恭, 山田典栄, 高橋秀明, 奥瀬千晃, 四柳宏, 伊東文生
    • Organizer
      日本消化器病学会総会
    • Place of Presentation
      京王プラザホテル(東京)
    • Related Report
      2012 Research-status Report
  • [Presentation] PLC/PRF/5細胞株におけるB型肝炎ウイルスDNAの新しい検出法2012

    • Author(s)
      平石哲也, 渡邊嘉行, 及川律子, 重福隆太, 大石嘉恭, 山田典栄, 高橋秀明, 奥瀬千晃, 四柳宏, 伊東文生
    • Organizer
      日本分子腫瘍マーカー研究会
    • Place of Presentation
      さっぽろ芸文館(札幌)
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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