| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Incretin is a naturally occurring peptide secreted by the L or K cells of the small intestine. Incretin regulates glucose-mediated insulin and glucagon productions by pancreatic beta and alpha cells. In this study, we demonstrated that incretin has a cognate receptors on human and mouse hepatocytes and that incretin has a direct effect on the reduction of hepatic steatosis in the absence of insulin. Incretin increased the phosphorylation of PDK-1, AKT, and protein kinase C in HepG2 and Huh7 cells. Incretin-treated hepatocytes resulted in a significant increase in cAMP production as well as reduction in mRNA expression of fatty acid synthesis-related genes. Finally, incretin treatment reversed hepatis statosis in mouse, but little effect occurred in hepatic fibrosis and cell proliferation. In conclusion, our data suggest that incretin has a novel direct effect on hepatocytes glucose and fat metabolism.
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