Analysis of hepatitis B virus in patients after liver transplantation

• Project/Area Number: 23590972
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyoto University
• Principal Investigator: UEDA Yoshihide 京都大学, 医学(系)研究科(研究院), 講師 (90378662)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: B型肝炎 / 肝移植 / 免疫抑制

Research Abstract

Hepatitis B virus (HBV) infected in individuals who are negative for hepatitis B surface antigen (HBsAg) but positive for hepatitis B core antibody (anti-HBc), which is indicative of resolved hepatitis B, was analyzed. De novo activation of HBV frequently occurred after liver transplantation from HBsAg-negative and anti-HBc-positive donors due to the emergence of HBV with escape mutations from hepatitis B surface antibody. Moreover, ultra-deep sequencing revealed that the genetic heterogeneity of reactivated HBV in immunosuppressive condition was significantly lower in patients with reactivation from occult HBV carrier status compared with that in patients from HBsAg carrier status.

Research Products

• [Journal Article] Reactivation from occult HBV carrier status is characterized by low genetic heterogeneity with the wild-type or G1896A variant prevalence (2014)
  • Author(s): Inuzuka T, Ueda Y, Morimura H, Fujii Y, Umeda M, Kou T, Osaki Y, Uemoto S, Chiba T, Marusawa H.
  • Journal Title: Journal of Hepatology Volume: 61 Issue: 3 Pages: 492-501
  • DOI: 10.1016/j.jhep.2014.04.033
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chronic rejection associated with antiviral therapy for recurrent hepatitis C after living-donor liver transplantation (2014)
  • Author(s): Ueda Y, Kaido T, Ito T, Ogawa K, Yoshizawa A, Fujimoto Y, Mori A, Miyagawa-Hayashino A, Haga H, Marusawa H, Chiba T, Uemoto
  • Journal Title: Transplantation Volume: 97 Issue: 3 Pages: 344-350
  • DOI: 10.1097/01.tp.0000435702.61642.0a
  • Peer Reviewed
• [Journal Article] Plasma cell hepatitis induced by the termination of antiviral therapy for recurrent hepatitis C after living donor liver transplantation (2013)
  • Author(s): Ueda Y, Yoshizawa A, Ogura Y, Miyagawa-Hayashino A, Haga H, Chiba T, Uemoto S
  • Journal Title: Hepatol Res Volume: (in press) Issue: 10
  • DOI: 10.1111/hepr.12243
  • Peer Reviewed
• [Journal Article] Efficacy and safety of prophylaxis with entecavir and hepatitis B immunoglobulin in preventing hepatitis B recurrence after living donor liver transplantation (2013)
  • Author(s): Ueda Y, Marusawa H, Kaido T, Ogura Y, Ogawa K, Yoshizawa A, Hata K, Fujimoto Y, Nishijima N, Chiba T, Uemoto S
  • Journal Title: Hepatol Res Volume: 43 Issue: 1 Pages: 67-71
  • DOI: 10.1111/j.1872-034x.2012.01020.x
  • NAID: 10031183459
• [Journal Article] Pretransplant serum hepatitis C virus RNA levels predict response to antiviral treatment after living donor liver transplantation (2013)
  • Author(s): Ueda Y, Kaido T, Ogura Y, Ogawa K, Yoshizawa A, Hata K, Fujimoto Y, Miyagawa-Hayashino A, Haga H, Marusawa H, Teramukai S, Uemoto S, Chiba T
  • Journal Title: PLoS One Volume: 8(3) Issue: 3 Pages: e58380-e58380
  • DOI: 10.1371/journal.pone.0058380
  • NAID: 120005244568
• [Journal Article] Dynamics of defective hepatitis C virus clones in reinfected liver grafts in liver transplant recipients; ultra-deep sequencing analysis (2013)
  • Author(s): Ohtsuru S, Ueda Y, Marusawa H, Inuzuka T, Nishijima N, Nasu A, Shimizu K, Koike K, Uemoto S, Chiba T
  • Journal Title: J Clin Microbiol Volume: 51(11) Issue: 11 Pages: 3645-3652
  • DOI: 10.1128/jcm.00676-13
• [Journal Article] 移植とウイルス肝炎 (2013)
  • Author(s): 上田 佳秀
  • Journal Title: 診断と治療 Volume: 101 Pages: 1357-1362
• [Journal Article] Effect of maintenance therapy with low-dose peginterferon for recurrent hepatitis C after living donor liver transplantation (2012)
  • Author(s): Ueda Y, Marusawa H, Kaido T, Ogura Y, Oike F, Mori A, Ogawa K, Yoshizawa A, Hatano E, Miyagawa-Hayashino A, Haga H, Egawa H, Takada Y, Uemoto S, Chiba T
  • Journal Title: J Viral Hepat Volume: 19 Issue: 1 Pages: 32-38
  • DOI: 10.1111/j.1365-2893.2010.01398.x
  • Peer Reviewed
• [Journal Article] Dynamics of Hepatitis B Virus Quasispecies in Association with Nucleos(t)ide Analogue Treatment Determined by Ultra-Deep Sequencing. (2012)
  • Author(s): Nishijima N
  • Journal Title: PLoS One Volume: 7 Issue: 4 Pages: 35052-35052
  • DOI: 10.1371/journal.pone.0035052
  • Peer Reviewed
• [Journal Article] 肝胆膵領域のEBM Update 2012 肝移植 (2012)
  • Author(s): 上田 佳秀
  • Journal Title: 肝胆膵 Volume: 64 Pages: 367-371
• [Journal Article] 臨床検査の分子生物学第14回 抗がん剤、免疫調節薬投与中の肝炎ウイルスマーカーの測定の意義 (2012)
  • Author(s): 上田 佳秀
  • Journal Title: 分子消化器病 Volume: 9 Pages: 49-53
• [Journal Article] 肝移植の適応と移植後の抗ウイルス療法 (2012)
  • Author(s): 上田 佳秀
  • Journal Title: 医学のあゆみ Volume: 240(9) Pages: 799-803
• [Journal Article] De novo activation of HBV with escape mutations from hepatitis B surface antibody after living donor liver transplantation (2011)
  • Author(s): Ueda Y, Marusawa H, Egawa H, Okamoto S, Ogura Y, Oike F, Nishijima N, Takada Y, Uemoto S, Chiba T
  • Journal Title: Antivir Ther Volume: 16(4) Issue: 4 Pages: 479-487
  • DOI: 10.3851/imp1771
  • Peer Reviewed
• [Journal Article] Genetic heterogeneity of hepatitis C virus in association with antiviral therapy determined by ultra-deep sequencing (2011)
  • Author(s): Nasu A, Marusawa H, Ueda Y, Nishijima N, Takahashi K, Osaki Y, Yamashita Y, Inokuma T, Tamada T, Fujiwara T, Sato F, Shimizu K, Chiba T
  • Journal Title: PLoS One Volume: 6 Issue: 9 Pages: e24907-e24907
  • DOI: 10.1371/journal.pone.0024907
  • NAID: 120003437134
  • Peer Reviewed
• [Journal Article] HBV変異株 核酸アナログ耐性変異とHBs抗体エスケープ変異 (2011)
  • Author(s): 西島 規浩、上田 佳秀
  • Journal Title: 今日の移植 Volume: 24(1) Pages: 90-95
• [Presentation] 臓器移植後の肝炎ウイルス対策の現状と問題点 (2013)
  • Author(s): 上田佳秀、千葉勉、上本伸二
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
  • Year and Date: 2013-09-07
• [Presentation] 肝移植後HBV対策の現状と問題点 (2012)
  • Author(s): 上田佳秀、吉澤淳、海道利実、小倉靖弘、岡本晋弥、小川晃平、秦浩一郎、藤本康弘、伊藤孝司、丸澤宏之、宮川文、羽賀博典、千葉勉、上本伸二
  • Organizer: 第30回日本肝移植研究会
  • Place of Presentation: 福岡
  • Year and Date: 2012-06-14
• [Presentation] Safety and efficacy of prophylaxis with entecavir and hepatitis B immunoglobulin in preventing hepatitis B recurrence after living donor liver transplantation (2011)
  • Author(s): Yoshihide Ueda, Hiroyuki Marusawa, Toshimi Kaido, Yasuhiro Ogura, Kohei Ogawa, Atsushi Yoshizawa, Koichiro Hata, Tsutomu Chiba, Shinji Uemoto
  • Organizer: The American Association for the Study of Liver Diseases 62th annual meeting
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2011-11-05
• [Presentation] 肝移植における肝臓内科医の役割 (2011)
  • Author(s): 上田佳秀、海道利実、小倉靖弘、小川晃平、吉澤淳、秦浩一郎、八木真太郎、江川裕人、千葉勉、上本伸二
  • Organizer: 第47回日本移植学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-06
• [Presentation] HBs抗体エスケープ変異を有するHBVの自然発生頻度と病態形成への関与 (2011)
  • Author(s): 上田佳秀、西島規浩、丸澤宏之、上本伸二、千葉勉
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] HBs抗体エスケープ変異を有するHBVの自然発生頻度と病態形成への関与 (2011)
  • Author(s): 上田 佳秀、西島 規浩、丸澤 宏之、上本 伸二、千葉 勉
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: ホテルグランパシフィックLE DAIBA（東京都）
• [Presentation] HBVゲノム多様性の次世代ゲノムアナライザー解析 (2011)
  • Author(s): 西島 規浩、上田 佳秀、丸澤 宏之
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: ホテルグランパシフィックLE DAIBA（東京都）
• [Presentation] 肝移植における肝臓内科医の役割 (2011)
  • Author(s): 上田 佳秀、海道 利実、小倉 靖弘、小川 晃平、吉澤 淳、秦 浩一郎、八木 真太郎、江川 裕人、千葉 勉、上本 伸二
  • Organizer: 第47回日本移植学会総会
  • Place of Presentation: 仙台国際センター（宮城県）
• [Presentation] Preexisting resistant mutants and dynamics of resistant populations of HBV to nucleoside analogues determined by massively-parallel ultra-deep sequencing. (2011)
  • Author(s): Nishijima N, Marusawa H, Ueda Y, Nasu A, Takahashi K, Osaki Y, Uemoto S, Chiba T.
  • Organizer: The American Association for the Study of Liver Diseases 62th annual meeting
  • Place of Presentation: San Francisco, CA, USA.
• [Presentation] Safety and efficacy of prophylaxis with entecavir and hepatitis B immunoglobulin in preventing hepatitis B recurrence after living donor liver transplantation. (2011)
  • Author(s): Ueda, Y, Marusawa H, Kaido T, Ogura Y, Ogawa K, Yoshizawa A, Hata K, Chiba T, Uemoto S.
  • Organizer: The American Association for the Study of Liver Diseases 62th annual meeting
  • Place of Presentation: San Francisco, CA, USA.
• [Presentation] 臓器移植後の肝炎ウイルス対策の現状と問題点
  • Author(s): 上田 佳秀、千葉 勉、上本 伸二
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 国立京都国際会館(京都）
• [Presentation] 肝移植後HBV対策の現状と問題点
  • Author(s): 上田 佳秀
  • Organizer: 第30回日本肝移植研究会
  • Place of Presentation: ホテル日航福岡（福岡）
• [Presentation] HBs抗原陰性を示したHBV-DNA陽性肝細胞癌症例のウイルスゲノム解析
  • Author(s): 荒澤壮一
  • Organizer: 第16回肝臓学会大会
  • Place of Presentation: 神戸国際展示場（神戸）
• [Book] de novo B型肝炎HBV再活性化予防のために基礎知識 (2013)
  • Author(s): 上田佳秀, 他
  • Publisher: 医薬ジャーナル社
• [Book] de novo B型肝炎 HBV再活性化予防のために基礎知識 (2013)
  • Author(s): 持田 智、田中 純子、片山 惠子、松尾 順子、渡邊 綱正、飯島 沙幸、田中 靖人、四柳 宏、八橋 宏、神田 達郎、横須賀 収、梅村 武司、田中 榮司、中山 伸朗、井戸 章雄、竹原 徹郎、上田 佳秀、渡部 玲子、木崎 昌弘、楠本 茂、他
  • Total Pages: 175
  • Publisher: 医薬ジャーナル社
• [Book] 肝移植後の抗ウイルス治療.肝硬変のマネジメント改訂版 (2011)
  • Author(s): 上田佳秀, 他
  • Publisher: 医薬ジャーナル社
• [Book] 肝硬変のマネジメント 改訂版 (2011)
  • Author(s): 上田 佳秀
  • Publisher: 医薬ジャーナル社