| Project/Area Number |
23590980
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kumamoto University |
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Principal Investigator |
NAOE Hideaki 熊本大学, 医学部附属病院, 助教 (30599246)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Yutaka 熊本大学, 大学院生命科学研究部, 教授 (70235282)
YOKOMINE Kazunori 熊本大学, 医学部付属病院, 助教 (60530128)
FUJIMOTO Jirou 兵庫医科大学, 医学部, 教授 (90199373)
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| Co-Investigator(Renkei-kenkyūsha) |
SAYA Hideyuki 慶應義塾大学, 医学部, 教授 (80264282)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝臓学 / pathway解析 / 大腸癌 / 浸潤・転移 / 消化器癌 / 細胞骨格 / 細胞運動 |
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| Research Abstract |
Suppress the expression of Cdh1 with siRNA tequnique in colon cancer cell line. These cells revealed cytoskeletal abnormality with decreased actin filament.In the analysis of cell motility with the wound healing assay, Cdh1 suppresse cells showed high motility compared with the control cells.
Next, we generated heterogenous mice which one of the phosphorylation site of Cdh1 was suppressed with gene trap tequnique.Genotyping of offspring of these mice was following as wild type: hetero: homo=13:32:0. These results indicated that homo suppression of Cdh1 phosphorylation site was embryonic lethal event in mice.Now,we are studying about the timing of lethality and cause of death.
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