The study of clinical application for HCV with miRNA containing the modified exosomes derived from iPS cells
| Project/Area Number |
23590993
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Yoshiki 大阪市立大学, 医学(系)研究科(研究院), 准教授 (00397556)
UMEZAWA Akihiro 独立行政法人国立成育医療研究センター, 部長 (70213486)
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| Co-Investigator(Renkei-kenkyūsha) |
SUDO Katsuko 東京医科大学, 医学部, 兼任講師 (50126091)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エクソソーム / HCV / 分子標的治療 / exosome / microRNA |
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| Research Abstract |
It is necessary to develop drug delivery system (DDS) to give a treatment using miRNA which targets HCV. In this study we examined to apply exosome to DDS for miRNA. We purified the exosomes from the cells which produced GE11 with the partial aliment of the ligand for EGFR and gave the target cell specificity. We produced the exosomes contain miRNA to gene transfer into the exosome-producing cells and confirmed that it was included in purified exosomes. We confirmed that the exosomes specifically performed delivery to the xenografted human cancer cells in mice. So we showed possibility to apply to treatment of the HCV by the exosomes containing miRNA which targets HCV in this system.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells2012
Author(s)
Ohno S., Takanashi M., Sudo K., Ueda S., Ishida A., Matsuyama N., Fujita K., Mizitani T., Ohgi T., Ochiya T., et. al.
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Journal Title
Mol. Ther
Volume: 21(1)
Pages: 185-191
Related Report
Peer Reviewed
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[Journal Article] TLS-CHOP represses miR-486 expression, inducing upregulation of a metastasis regulator PAI-1 in human myxoid liposarcoma2012
Author(s)
N. Borjigin, S. Ohno, W. Wu, M. Tanaka, R. Suzuki, K. Fujita, M. Takanashi, K. Oikawa, T. Goto, T. Motoi, T. Kosaka, K. Yamamoto, M. Kuroda
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 427
Issue: 2
Pages: 355-360
DOI
Related Report
Peer Reviewed
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